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  4. Recombinant Mouse Patatin-like phospholipase domain-containing protein 3 (Pnpla3), partial

Recombinant Mouse Patatin-like phospholipase domain-containing protein 3 (Pnpla3), partial

  • 中文名称：
    小鼠Pnpla3重组蛋白
  • 货号：
    CSB-YP856310MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Pnpla3重组蛋白
  • 货号：
    CSB-EP856310MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Pnpla3重组蛋白
  • 货号：
    CSB-EP856310MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Pnpla3重组蛋白
  • 货号：
    CSB-BP856310MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Pnpla3重组蛋白
  • 货号：
    CSB-MP856310MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Pnpla3
  • Uniprot No.：
  • 别名：
    Pnpla3; Adpn; 1-acylglycerol-3-phosphate O-acyltransferase Pnpla3; EC 2.3.1.51; Acylglycerol transacylase; Adiponutrin; ADPN; Calcium-independent phospholipase A2-epsilon; iPLA2-epsilon; Lysophosphatidic acid acyltransferase; Patatin-like phospholipase domain-containing protein 3; EC 3.1.1.3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Specifically catalyzes coenzyme A (CoA)-dependent acylation of 1-acyl-sn-glycerol 3-phosphate (2-lysophosphatidic acid/LPA) to generate phosphatidic acid (PA), an important metabolic intermediate and precursor for both triglycerides and glycerophospholipids. Does not esterify other lysophospholipids. Acyl donors are long chain (at least C16) fatty acyl-CoAs: arachidonoyl-CoA, linoleoyl-CoA, oleoyl-CoA and at a lesser extent palmitoyl-CoA. Additionally possesses low triacylglycerol lipase and CoA-independent acylglycerol transacylase activities and thus may play a role in acyl-chain remodeling of triglycerides. Has hydrolytic activity against glycerolipids triacylglycerol, diacylglycerol and monoacylglycerol, with a strong preference for oleic acid as the acyl moiety.
  • 基因功能参考文献：
    1. Histone acetylation plays a role in the modulation of PNPLA3 levels in the liver exposed to binge ethanol drinking. PMID: 28433418
    2. 148M variant disrupts ubiquitylation and proteasomal degradation PMID: 28520213
    3. Neither ablation nor overexpression of wild-type PNPLA3 affects liver fat content in mice, whereas hepatic overexpression of the human 148M transgene causes steatosis. PMID: 24917523
    4. rs738409 exerts a marked influence on hepatocarcinogenesis in patients with cirrhosis of European descent. PMID: 24114809
    5. PNPLA3 plays a role in remodeling TAG in lipid droplets, as they accumulate in response to food intake PMID: 23023705
    6. adiponutrin/PNPLA3 is regulated by two key factors of the glycolytic and lipogenic pathways, raising the question of its implication in the metabolism of carbohydrates and lipids PMID: 21145868
    7. data argue against a role for Pnpla3 loss-of-function in fatty liver disease or metabolic syndrome in mice. PMID: 21068004
    8. Loss of Pnpla3 does not cause fatty liver, liver enzyme elevation, or insulin resistance in mice. PMID: 20648554
    9. Microarray-based gene profiling showed that the expression level of PNPLA3 in hepatocytes is correlated with that of genes associated with the lipogenic pathway such as ME1, SPOT14, and SCD1. PMID: 20015565
    10. data suggest that murine ATGL but not adiponutrin contributes to net adipocyte lipolysis and that ATGL and adiponutrin are oppositely regulated by insulin both in vitro and in vivo PMID: 16380488

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  • 亚细胞定位：
    Membrane; Single-pass membrane protein. Lipid droplet.
  • 组织特异性：
    Restricted to adipose tissue. Expressed in inguinal and epididymal white adipose tissues and in interscapular brown adipose tissue. Also expressed in liver in response to high-sucrose diet.
  • 数据库链接：

    KEGG: mmu:116939

    STRING: 10090.ENSMUSP00000043826

    UniGene: Mm.390817