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  4. Recombinant Mouse Nuclear transcription factor Y subunit alpha (Nfya)

Recombinant Mouse Nuclear transcription factor Y subunit alpha (Nfya)

  • 中文名称：
    小鼠Nfya重组蛋白
  • 货号：
    CSB-YP015773MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Nfya重组蛋白
  • 货号：
    CSB-EP015773MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Nfya重组蛋白
  • 货号：
    CSB-EP015773MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Nfya重组蛋白
  • 货号：
    CSB-BP015773MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Nfya重组蛋白
  • 货号：
    CSB-MP015773MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Nfya; Nuclear transcription factor Y subunit alpha; CAAT box DNA-binding protein subunit A; Nuclear transcription factor Y subunit A; NF-YA
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-346
  • 氨基酸序列
    MEQYTTNSNS STEQIVVQAG QIQQQQGGVT AVQLQTEAQV ASASGQQVQT LQVVQGQPLM VQVSGGQLIT STGQPIMVQA VPGGQGQTIM QVPVSGTQGL QQIQLVPPGQ IQIQGGQAVQ VQGQQGQTQQ IIIQQPQTAV TAGQTQTQQQ IAVQGQQVAQ TAEGQTIVYQ PVNADGTILQ QVTVPVSGMI TIPAASLAGA QIVQTGANTN TTSSGQGTVT VTLPVAGNVV NSGGMVMMVP GAGSVPAIQR IPLPGAEMLE EEPLYVNAKQ YHRILKRRQA RAKLEAEGKI PKERRKYLHE SRHRHAMARK RGEGGRFFSP KEKDSPHMQD PNQADEEAMT QIIRVS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. NF-YA positively regulates the transcription of the core clock component ARNTL/BMAL1.
  • 基因功能参考文献：
    1. Data show that nuclear factor Y (NF-Y) binds to the inverted CCAAT element (ICE) in the Fatty acid synthase (Fasn) promoter specifically in refeeding states. PMID: 28281280
    2. findings may point to a possible role of NF-YA in stress conditions that occur in chronic obesity, ER stress might be involved in the pathogenesis of obesity through NF-YA depletion. PMID: 27837431
    3. we conclude that NF-Y and YY1 are important for the basal transcription of Pcyt2 and that NF-Y is involved in the inhibitory effects of 25-HC on Pcyt2 transcription. PMID: 26310456
    4. these results indicate that the transcription factor NF-Y regulates the proximal promoter activity of mouse Col11a1 gene in chondrocytes PMID: 24092017
    5. these results raise an exciting possibility that targeting CDK1 or NF-Y in the diseased heart may inhibit fibrosis and subsequently confer cardioprotection. PMID: 24477232
    6. NF-Ya is a functional activator of Bmal1 transcription and it cooperates with Sp1 and Rev-Erbalpha to generate the daily cycle of Bmal1 expression. PMID: 24030830
    7. NF-Y belongs to the restricted circle of transcription factors that govern mouse embryonic stem cells, and that NF-YAs is the active isoform in these cells. PMID: 22969033
    8. Sp1, CREB, HNF-1, and NF-Y, known to be responsive to hormones and diet, regulate NAGS transcription PMID: 22383952
    9. results indicate nuclear transcription factor NF-Y as a pivotal upstream participant in a regulatory network necessary for the preservation of cycling hematopoietic stem cell. PMID: 22072554
    10. Findings establish that NF-Y acts upstream of E2F1 in p53-mediated apoptosis. PMID: 20952509
    11. Transcriptional regulation of myeloid differentiation primary response (MyD) genes during myeloid differentiation is mediated by nuclear factor Y. PMID: 12070012
    12. NF-Y recruits RNAPII and general transcription factors onto various CCAAT box-containing promoters to permit strong transcriptional activation independently of histone modifications PMID: 15601870
    13. NF-Y can stimulate or repress the FGF-4 promoter in an enhancer-dependent manner PMID: 16128003
    14. NF-Y activation by TGF-beta1 involves ERK1/2 and potentially an interplay between MAPK pathways, thereby opening the possibility for finely tuned transcriptional regulation PMID: 16434965
    15. NF-Y binds to the promoter and/or within the coding region. NF-Y is embedded in positive & negative methyl histone marks, serving a dual function in transcriptional regulation, as an activator or as a repressor. PMID: 18212061
    16. Sequestration of NF-YA component to mutant huntingtin aggregates in model mouse brain results in the reduction of HSP70 gene transcription. PMID: 18288205
    17. These data indicate a role of NF-Y in modulating the structure and transcriptional competence of chromatin. PMID: 18431504
    18. NF-Y is a fundamental switch at the heart of decision between gene activation and repression in CCAAT regulated genes. PMID: 18446193
    19. results demonstrate that interaction of NF-Y at the CCAAT box is pivotal to FGFR2 gene transcription partly through the construction of a local open chromatin configuration across the promoter PMID: 19047043

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    NFYA/HAP2 subunit family
  • 数据库链接：

    KEGG: mmu:18044

    STRING: 10090.ENSMUSP00000043909

    UniGene: Mm.4929