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  4. Recombinant Mouse Nuclear receptor subfamily 5 group A member 2 (Nr5a2)

Recombinant Mouse Nuclear receptor subfamily 5 group A member 2 (Nr5a2)

  • 中文名称：
    小鼠Nr5a2重组蛋白
  • 货号：
    CSB-EP016066MO
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    Nr5a2
  • Uniprot No.：
  • 别名：
    Nr5a2; Lrh1; Nuclear receptor subfamily 5 group A member 2; Liver receptor homolog 1; LRH-1
  • 种属：
    Mus musculus(Mouse)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    71.5 kDa
  • 表达区域：
    1-560aa
  • 氨基酸序列
    MSASLDTGDFQEFLKHGLTAIASAPGSETRHSPKREEQLREKRAGLPDRHRRPIPARSRLVMLPKVETEAPGLVRSHGEQGQMPENMQVSQFKMVNYSYDEDLEELCPVCGDKVSGYHYGLLTCESCKGFFKRTVQNQKRYTCIENQNCQIDKTQRKRCPYCRFKKCIDVGMKLEAVRADRMRGGRNKFGPMYKRDRALKQQKKALIRANGLKLEAMSQVIQAMPSDLTSAIQNIHSASKGLPLSHVALPPTDYDRSPFVTSPISMTMPPHSSLHGYQPYGHFPSRAIKSEYPDPYSSSPESMMGYSYMDGYQTNSPASIPHLILELLKCEPDEPQVQAKIMAYLQQEQSNRNRQEKLSAFGLLCKMADQTLFSIVEWARSSIFFRELKVDDQMKLLQNCWSELLILDHIYRQVAHGKEGTIFLVTGEHVDYSTIISHTEVAFNNLLSLAQELVVRLRSLQFDQREFVCLKFLVLFSSDVKNLENLQLVEGVQEQVNAALLDYTVCNYPQQTEKFGQLLLRLPEIRAISKQAEDYLYYKHVNGDVPYNNLLIEMLHAKRA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer，50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Nuclear receptor that acts as a key metabolic sensor by regulating the expression of genes involved in bile acid synthesis, cholesterol homeostasis and triglyceride synthesis. Together with the oxysterol receptors NR1H3/LXR-alpha and NR1H2/LXR-beta, acts as an essential transcriptional regulator of lipid metabolism. Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor: inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development. Activates the transcription of CYP2C38.
  • 基因功能参考文献：
    1. LRH-1 agonism favors an immune cells-islet dialogue which protects against diabetes mellitus. PMID: 29662071
    2. potent suppressor of neural stem cell self-renewal and key player in neural fate decisions through direct regulatory effects on critical genes and pathways PMID: 27447294
    3. transcriptional regulation by NR5A2 links differentiation and inflammation in the pancreas; findings support the notion that, in the pancreas, the transcriptional networks involved in differentiation-specific functions also suppress inflammatory programs; under conditions of genetic or environmental constraint, these networks can be subverted to foster inflammation PMID: 29443959
    4. The data identify the key role of biliary phospholipids in sustaining intestinal mucosa proliferation and tumor progression through the activation of nuclear receptor Lrh1. PMID: 27995969
    5. It was concluded that Nr5a2 is essential for cumulus expansion in granulosa cells throughout follicular development. PMID: 28520915
    6. These findings suggest that compromised SUMOylation of LRH-1 promotes the development of nonalcoholic fatty liver disease under lipogenic conditions through regulation of OSBPL3. PMID: 28094767
    7. these data show for the first time LRH-1 expression in T cells, its role in FASLG transcription and the potential of pharmacological inhibition of LRH-1 in the treatment of FasL-mediated immunopathologies PMID: 28406481
    8. highlight the importance of LRH-1 in coordinating glutamine-induced metabolism and signaling to promote hepatocellular carcinogenesis. PMID: 27298334
    9. Mice carrying a mutation on lysine 289 of LRH-1 (Lrh1 K289R mice) display reduced LRH-1 SUMOylation and increased expression of genes regulating cholesterol transport. PMID: 25176150
    10. Results identify LRH-1 as a critical component of the anti-inflammatory and fungicidal response of alternatively activated macrophages that acts upstream from the IL-13-induced 15-HETE/PPARgamma axis. PMID: 25873311
    11. The s conclude that LRH-1 initiates a novel pathway of endoplasmic reticulum stress resolution that is independent of the unfolded protein response, yet equivalently required. PMID: 24737860
    12. study demonstrates for the first time that LRH-1 has a CRT-dependent NES which is not only required for cytoplasmic trafficking, but also essential for correct protein folding to avoid misfolding-induced aggregation. PMID: 26268559
    13. these data demonstrate that copper-mediated nuclear receptor dysfunction disrupts liver function in WD and potentially in other disorders associated with increased hepatic copper levels. PMID: 26241054
    14. Heterozygous gene deletion of LRH-1 causes body weight gains without any apparent worsening of glucose and lipid metabolism. PMID: 24531913
    15. NR5A2 has a role in controlling aspects of multipotent progenitor cell formation and acinar differentiation during pancreatic organogenesis PMID: 25063451
    16. Report fully reversible infertility phenotype of LRH-1-knockdown mice. PMID: 23425349
    17. this study shows that the combination of Rarg and Nr5a2 rapidly promote the iN cell maturation within 1 week and greatly facilitate the conversion with neuronal purities of approximately 50% and yields of >130%. PMID: 24459144
    18. Data (including data from transgenic overexpression/gene silencing) suggest that Nr5a2 modulates signal transduction/cell proliferation in mammary cells; mammary morphology exhibits significant reduction in lateral budding after Nr5a2 overexpression. PMID: 24564400
    19. Data (including data from various knockout mice strains) suggest that Nr5a2 differentially regulates female fertility across the trajectory of follicular development especially in later stages of follicle development. PMID: 24552399
    20. FTF combined with CYP7A might play an important role in Lrh1 regulated pancreas-specific transcriptional network. PMID: 24735206
    21. regulates pancreatic acinar plasticity and constrains pancreatic neoplasia driven by oncogenic Kras PMID: 23645620
    22. NR5A2 can act in a non-redundant manner to regulate Lhb and Fshb transcription in vitro, but is dispensable in vivo. PMID: 23536856
    23. Lrh-1 is necessary for maintenance of the corpus luteum, for promotion of decidualization and for formation of the placenta. PMID: 23817023
    24. Data indicate that sequence divergence has differentially impacted ligand binding and protein dynamics in NR5A2. PMID: 23737522
    25. Liver receptor homolog-1 (LRH-1), a member of the nuclear receptor superfamily, is expressed in kisspeptin neurons in the arcuate nucleus but not in the anteroventral periventricular in female mice. PMID: 23504956
    26. in conditional deletion of Lrh1 in liver, analysis of hepatic glucose fluxes revealed reduced glucokinase and glycogen synthase fluxes as compared with those of wild-type littermates PMID: 22772466
    27. LRH-1 recruits FXR to lipid metabolic genes. PMID: 22296850
    28. findings demonstrate that signaling through RARs has critical roles in molecular reprogramming and that the synergistic interaction between Rarg and Lrh1 directs reprogramming toward ground-state pluripotency PMID: 21990348
    29. Data show that LRH-1 is a key regulator of the exocrine pancreas-specific transcriptional network required for the production and secretion of pancreatic fluid. PMID: 21852532
    30. Liver receptor homolog-1 is critical for adequate up-regulation of Cyp7a1 gene transcription and bile salt synthesis during bile salt sequestration. PMID: 21391220
    31. findings identify an LRH-1 dependent phosphatidylcholine signalling pathway that regulates bile acid metabolism and glucose homeostasis PMID: 21614002
    32. Data characterize two functional nuclear localization signals in LRH-1 and show that three crucial basic clusters are involved in the DNA binding and transcriptional activities of LRH-1. PMID: 20853131
    33. These data indicate that LRH-1 and Nanog cooperate to regulate Dax1 expression in mouse embryonic stem cells. PMID: 20937355
    34. through interaction with LRH-1 and transcriptional activation of Oct4 and other genes PMID: 20943815
    35. Lrh-1 was identified as a novel beta-catenin target gene, and Lrh-1 regulation is required for maintaining proper levels of Oct4, Nanog, and Tbx3. PMID: 20734354
    36. show that the orphan nuclear receptor Nr5a2 (also known as Lrh-1) can replace Oct4 in the derivation of iPSCs from mouse somatic cells, and it can also enhance reprogramming efficiency. PMID: 20096661
    37. role in mediating species- and cell line-specific bile acid-dependent negative feedback regulation of the apical sodium-dependent bile acid transporter PMID: 12456679
    38. the specificities of LXXLL-related motifs in Dax-1 based on their amino acid sequences play an important role in regulation of orphan receptors. PMID: 12482977
    39. up-regulation of Lrh-1 and Mrp3(Abcc3) reduces liver injury in obstructive cholestasis PMID: 12837754
    40. LRH-1 was expressed in estrogen biosynthesis-granulosa cells during the estrous cycle, and in corpora lutea of pregnancy and was proposed playing an important role as a competence factor in regulating aromatase and estrogen biosynthesis in ovary. PMID: 12972182
    41. The FTF-beta galactosidase mouse establishes a central role for FTF in developmental, nutritive, and metabolic functions from early embryogenesis through adulthood. PMID: 15014077
    42. Results show that LRH-1 induces cell proliferation through the concomitant induction of cyclin D1 and E1, an effect that is potentiated by its interaction with beta-catenin. PMID: 15327767
    43. LRH-1 plays an essential role in the maintenance of Oct4 expression in ES cells at the epiblast stage of embryonic development. PMID: 15831456
    44. Roles in gonadal development, the initiation of folliculogenesis and regulation of estrogen biosynthesis within the ovary. PMID: 16003771
    45. LRH-1 is strongly expressed throughout the mouse brain suggesting important roles for this transcription factor PMID: 16264403
    46. FTF and LRH-1 are two related but different transcription factors in human Caco-2 cells, suggesting that they may be homologues and not orthologues. PMID: 16469397
    47. mlrh-1v2 expression is directed by a new promoter which displays a strong activity in ES cells PMID: 16500646
    48. LRH-1 is a new transcriptional partner for GATA factors in the regulation of inhibin alpha-subunit gene expression. PMID: 16893604
    49. These results demonstrate a novel role for liver receptor homologue-1 (LRH-1) in the regulation of intestinal glucocorticoid synthesis and propose LRH-1 as an important regulator of intestinal tissue integrity and immune homeostasis. PMID: 16923850
    50. LRH-1 is a key player in the control of the hepatic acute-phase response. PMID: 16943422

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Nuclear hormone receptor family, NR5 subfamily
  • 数据库链接：

    KEGG: mmu:26424

    STRING: 10090.ENSMUSP00000027649

    UniGene: Mm.16794