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  4. Recombinant Mouse Nuclear receptor subfamily 1 group I member 3 (Nr1i3)

Recombinant Mouse Nuclear receptor subfamily 1 group I member 3 (Nr1i3)

  • 中文名称：
    小鼠Nr1i3重组蛋白
  • 货号：
    CSB-YP016049MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Nr1i3重组蛋白
  • 货号：
    CSB-EP016049MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Nr1i3重组蛋白
  • 货号：
    CSB-EP016049MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Nr1i3重组蛋白
  • 货号：
    CSB-BP016049MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Nr1i3重组蛋白
  • 货号：
    CSB-MP016049MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Nr1i3
  • Uniprot No.：
  • 别名：
    Nr1i3; Car; Nuclear receptor subfamily 1 group I member 3; Constitutive androstane receptor; CAR; Orphan nuclear receptor MB67
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-358
  • 氨基酸序列
    MTAMLTLETM ASEEEYGPRN CVVCGDRATG YHFHALTCEG CKGFFRRTVS KTIGPICPFA GRCEVSKAQR RHCPACRLQK CLNVGMRKDM ILSAEALALR RARQAQRRAE KASLQLNQQQ KELVQILLGA HTRHVGPMFD QFVQFKPPAY LFMHHRPFQP RGPVLPLLTH FADINTFMVQ QIIKFTKDLP LFRSLTMEDQ ISLLKGAAVE ILHISLNTTF CLQTENFFCG PLCYKMEDAV HAGFQYEFLE SILHFHKNLK GLHLQEPEYV LMAATALFSP DRPGVTQREE IDQLQEEMAL ILNNHIMEQQ SRLQSRFLYA KLMGLLADLR SINNAYSYEL QRLEELSAMT PLLGEICS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds and transactivates the retinoic acid response elements that control expression of the retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes. Transactivates both the phenobarbital responsive element module of the human CYP2B6 gene and the CYP3A4 xenobiotic response element.
  • 基因功能参考文献：
    1. Study uncovered a novel function of CAR in mediating the kidney-liver cross-talk in acute kidney injury (AKI). Renal IR downregulated the expression and activity of CAR and decreased the expression of microsomal triglyceride transfer protein (MTTP), which resulted in a decreased very-low-density lipoprotein triglyceride (VLDL-TG) secretion and lipid accumulation and liver injury. PMID: 29351922
    2. The role of the CAR signaling pathways within testis was validated using specific CAR agonist (TCPOBOP) and inverse agonist (androstanol) that respectively inhibited or reproduced the phenotype observed in Fxralpha-/- males fed Bile acids (BAs)-diet. These data open interesting perspectives to better define how BA homeostasis contributes to physiological or pathophysiological conditions via the modulation of CAR activity. PMID: 28181583
    3. data provide a comprehensive view of the CAR-regulated transcriptome and give insight into the mechanism of sex-biased susceptibility to CAR-dependent mouse liver tumorigenesis PMID: 28903501
    4. FXR signaling is a bile acid nuclear receptor that regulates lipids and glucose homeostasis and lack of it causes hepatomegaly and liver dysfunction. PMID: 29142166
    5. These results revealed the bilirubin transport regulatory mechanisms and highlighted the importance of CAR in modulating the bilirubin clearance pathway in the ALD mouse model. PMID: 28393244
    6. Activation of constitutive androstane receptor results in maintained biliary excretion of bile acids despite a marked decrease of bile acids in liver. PMID: 26984780
    7. CAR plays an important role in acifluorfen-induced liver injury and tumor development in mice. PMID: 26928356
    8. Activation of the nuclear receptor constitutive androstane receptor (CAR) induced FNDC5 mRNA expression in the liver. PMID: 27007446
    9. CAR is important for hepatic clearance of several widely prescribed drugs metabolized by P450 enzymes, however the fasting-induced alterations in P450-mediated drug clearance are largely independent of CAR. PMID: 27434302
    10. A functional network among CAR targeted genes and the affected microRNAs was constructed to illustrate how CAR modulation of microRNA expression may potentially mediate its biological role in mouse hepatocyte proliferation. PMID: 27080131
    11. The present study has revealed known and novel, as well as common and unique targets of PXR and CAR in mouse liver following pharmacological activation using their prototypical ligands. PMID: 27113289
    12. Results show that circadian disruption activates CAR via sympathetic dysfunction and cholestasis. The nuclear receptor CAR drives NAFLD to NASH, fibrosis, and hepatocellular carcinoma progression PMID: 27889186
    13. These data provide new insights into the regulation by CAR of glycolytic and lipogenic genes and on pathogenesis of steatosis PMID: 27180240
    14. CAR plays an important role in maintaining the homeostasis of cholesterol, bile acids, and triglycerides levels. PMID: 28283178
    15. CAR activation initiates a transcriptional program that facilitates the coordinated metabolic activities required for cell proliferation. PMID: 27530923
    16. This study demonstrated that Lack of CAR impacts neuronal function and cerebrovascular integrity in mice. PMID: 27240521
    17. CAR pathway was the main mechanism of liver tumor development induced by imazalil. PMID: 27853109
    18. data indicate that DE might be a potential therapeutic agent for obese pregnant patients with insulin resistance through CAR to prevent the perinatal outcomes such as preeclampsia, gestational diabetes, and macrosomia. Further analysis of possible complications and side effects using animal models is required. PMID: 27426490
    19. The negative regulation of PGC1alpha by CAR may represent a cellular adaptive mechanism to accommodate energy-restricted conditions. PMID: 26407237
    20. Data (including data from studies in knockout mice) suggest that CAR plays role in liver hypertrophy and hepatocarcinogenesis due to Ginkgo biloba extract used as dietary supplement in China; data are from subchronic toxicity tests. PMID: 26115596
    21. Combining CAR activation with limited beta-catenin activation induces tumorigenesis, and the tumours share a conserved gene expression signature with beta-catenin-positive human hepatocellular carcinoma. PMID: 25661872
    22. Constitutive androstane receptor is the main mediator of liver hypertrophy induced by cyproconazole and fluconazole, but not tebuconazole; constitutive androstane receptor played a crucial role in liver tumor development induced by all three triazoles. PMID: 25656644
    23. CAR activation decreases miR-122 levels through suppression of HNF4alpha transcriptional activity and indirectly regulates the promitogenic protein cMyc. PMID: 26171734
    24. The induction of CYP2B by PB or CFA was comparable to nuclear CAR levels. CAR nuclear translocation was induced by CFA in both rat strains. PMID: 25052003
    25. The nuclear receptor constitutive androstane receptor/NR1I3 enhances the profibrotic effects of transforming growth factor beta and contributes to the development of experimental dermal fibrosis. PMID: 25155144
    26. ubiquitin-proteasomal regulation of CCRP and HSP70 are important contributors to the regulation of cytoplasmic CAR levels, and hence the ability of CAR to respond to PB or PB-like inducers PMID: 24789201
    27. Data indicate that in the CAR nuclear receptor (CAR)-deficient mice, fatty liver was observed not only in the ethanol group but also with the ethanol plus polyphenol groups. PMID: 24498295
    28. These results suggest that in vivo deletion of CAR resulted in higher bone mass, which appears to be a result from reduced metabolism of testosterone due to down-regulation of Cyp2b. PMID: 24114688
    29. Studies indicate that phenobarbital binding to EGFR inhibits distal signaling to derepress RACK1-facilitated dephosphorylation of constitutive active androstane receptor (CAR) by PP2A, and activates the transcription of Cyp2B10. PMID: 23652202
    30. Data indicate that the phosphorylation status of constitutive active androstane receptor (CAR) and EGFR in response to phenobarbital and EGF was confirmed in hepatocytes. PMID: 23652203
    31. Xenobiotic-induced hepatocyte proliferation mediated by CAR or PPARalpha is enhanced by PXR co-activation despite the fact that PXR activation alone does not cause the cell proliferation in mouse livers. PMID: 23626729
    32. The constitutive androstane receptor is involved by a dose-response relationship in mouse liver hypertrophy induced by triazole fungicides. PMID: 23721867
    33. Neonatal activation of CAR results in epigenetic memory and a permanent change of liver drug metabolism. PMID: 22488010
    34. AhR, CAR, PXR, PPARalpha, and Nrf2 regulate genes encoding drug-metabolizing enzymes and transporters in livers of mice after chemical activation. PMID: 22496397
    35. phenobarbital-mediated down-regulation of miR-122 is an early and important event in the AMPK-dependent CAR activation and transactivation of its target genes PMID: 22815988
    36. CAR mRNA expression was increased by hypothyroidism PMID: 22503787
    37. CAR and PXR are closely associated and have diverse roles, both as positive and negative regulators of hepatic genes. PMID: 22698814
    38. SRC-3 is the predominant transcriptional co-activator among the three SRC family members for CAR activation to promote hepatocyte proliferation and drug metabolism. PMID: 21827731
    39. Nuclear receptor CAR (NR1I3) is essential for DDC-induced liver injury and oval cell proliferation in mouse liver. PMID: 21826054
    40. Hepatocyte-specific knockout of beta-catenin inhibited CAR agonists-induced hepatocyte proliferation in male mice. PMID: 21705713
    41. activated ERK1/2 interacts with CAR and represses dephosphorylation of Thr-38, providing a cell signal-regulated mechanism for CAR activation. PMID: 21873423
    42. CAR is involved in the control of cholesterol and bile acid homeostasis, increasing reverse cholesterol transport under hyperlipidemic conditions. PMID: 21145854
    43. Constitutive androstane receptor activation decreases plasma apolipoprotein B-containing lipoproteins and atherosclerosis in low-density lipoprotein receptor-deficient mice. PMID: 21778422
    44. role in modulating alcoholic liver injury PMID: 21519326
    45. FAM84A is up-regulated by CAR during the development of liver tumors. PMID: 21424122
    46. deletion of CAR leads to a significantly lower tumor incidence and smaller tumor diameter PMID: 21173431
    47. CAR may repress death of mouse primary hepatocytes by forming a GADD45B complex and repressing MKK7-mediated phosphorylation of JNK1 PMID: 20404936
    48. FXR activation in obstructive cholestasis might worsen liver injury by hijacking a protective mechanism regulated by CAR and provides a new molecular explanation to the pathophysiology of cholestasis. PMID: 21296199
    49. Helix 11 dynamics is critical for constitutive androstane receptor activity. PMID: 21220114
    50. Data observed that OSM positively augmented the CAR and UGT1A1 expressions and CAR-mediated signaling in vivo and in vitro, through cross talk between the nuclear CAR receptor and the plasma membrane OSM receptor, via the MAPK cascade. PMID: 20197307

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Cytoplasm, cytoskeleton. Note=Recruited to the cytoplasm by DNAJC7.
  • 蛋白家族：
    Nuclear hormone receptor family, NR1 subfamily
  • 组织特异性：
    Predominantly expressed in liver.
  • 数据库链接：

    KEGG: mmu:12355

    STRING: 10090.ENSMUSP00000005820

    UniGene: Mm.486506