Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse Neuroligin-3 (Nlgn3), partial

Recombinant Mouse Neuroligin-3 (Nlgn3), partial

  • 货号：
    CSB-YP803963MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP803963MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP803963MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP803963MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP803963MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Nlgn3
  • Uniprot No.：
  • 别名：
    Nlgn3; Neuroligin-3; Gliotactin homolog
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and probably mediates its effects by recruiting and clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. May also play a role in glia-glia or glia-neuron interactions in the developing peripheral nervous system.
  • 基因功能参考文献：
    1. male Nlgn3 knockout mice and their wild-type littermates displayed deficits in sociability, female mice lacking Nlgn3 were insensitive to their peers' behavior but modified the social behavior of their littermates. PMID: 28795135
    2. Data suggest an unanticipated mechanism of developmental compensation whereby cerebellin-1 and neuroligin-3 functionally occlude each other during development of calyx synapses. PMID: 27725662
    3. Nlgn3(R451C) knock-in mice, an established genetic model for autism spectrum disorders, exhibited normal duration and distribution of sleep/wake states but significantly altered electroencephalography (EEG) power spectral profiles for wake and sleep. PMID: 28385162
    4. The NL3 deletion-mutant mouse was a dramatic reduction of the number of GABAAalpha2-subunit containing GABAA receptor clusters at the retinal inner plexiform layer. PMID: 28708891
    5. that neuroligin-3 specifies the properties of excitatory synapses on parvalbumin-containing interneurons by a retrograde trans-synaptic mechanism and suggest a molecular pathway whereby neuroligin-3 mutations contribute to neuropsychiatric disorders PMID: 28067903
    6. Study shows altered mating behavior in the Neuroligin-3(R451C mutation) mouse and has provides new insights into the aggressive phenotype in this model. PMID: 28255463
    7. Results identify impaired tonic endocannabinoid signaling as a common mechanism of Nlgn3R451C-induced excitatory/inhibitory imbalance in cortex, consistent with that identified in hippocampus. PMID: 26469287
    8. Nlgn3 and Nrxn1 are differentially expressed in cerebral cortex and hippocampus which might be responsible for alternations in synaptic plasticity during aging. PMID: 25693924
    9. These findings suggest that structural changes in the brain of NL-3 mice are induced by the mutation in the NL-3 gene PMID: 25299583
    10. Thus our data suggest that perinatal exposure to BPA impairs spatial memory through upregulation of expression of synaptic proteins Nrxn1 and Nlgn3 and increased dendritic spine density in cerebral cortex and hippocampus of postnatal male mice. PMID: 25330104
    11. Therefore, we sought to replicate our findings in the neuroligin-3 R451C point mutant knock-in mouse model (NL3R451C) in a different genetic background. PMID: 24619977
    12. We used MRI to image three genetic mouse models with single mutations implicated in autism: Neuroligin-3 R451C knock-in, Methyl-CpG binding protein-2 (MECP2) 308-truncation and integrin beta3 homozygous knockout. PMID: 24151012
    13. Results indicate that autism spectrum disorders (ASD)-associated NL3 mutations commonly disrupt striatal circuits in a subregion-, cell-type-, and synapse-specific fashion and suggest that this disruption may shape repetitive and stereotypic behaviors associated with ASD. PMID: 24995986
    14. Neuroligin-3 R704C-mutant induced neurons exhibit a large and selective decrease in AMPA-type glutamate receptor-mediated synaptic transmission without changes in NMDA-type glutamate receptor- or in GABAA receptor-mediated synaptic transmission. PMID: 24046374
    15. Our data thus suggest that neuroligin-3 is specifically required for tonic endocannabinoid signaling PMID: 23583622
    16. in NL3(R451C) mutant mice, a single point mutation in a synaptic cell adhesion molecule causes context-dependent changes in synaptic transmission PMID: 21808020
    17. The NL-3 acts redundantly to maintain excitatory synapses and that synapse elimination caused by the absence of NL-3 is promoted by synaptic activity and mediated by a postsynaptic Ca(2+)/CaM-dependent signaling pathway. PMID: 21788371
    18. An autism-associated point mutation in the neuroligin cytoplasmic tail selectively impairs AMPA receptor-mediated synaptic transmission in hippocampus. PMID: 21642956
    19. exposure to valproic acid (VPA) on gestational day 11 in mice results in a long-term, global reduction of NLGN3 at the level of gene expression, this lowered expression may be linked to autistic-like behavioral phenotype observed in the VPA mice PMID: 19607885
    20. Data show that neuroligin 3 is required for proper synapse maturation and brain function, but not for the initial formation of synaptic contacts. PMID: 16982420
    21. R451C(Arg451-->Cys451 substitution implicated in autism) mutants had impaired social interactions but enhanced spatial learning ability; changes were accompanied by increased inhibitory synaptic transmission with no apparent effect on excitatory synapses PMID: 17823315
    22. NL-3-deficient mice display a behavioral phenotype reminiscent of the lead symptoms of autism spectrum disorder PMID: 19243448
    23. these data support the hypothesis that the autism-associated NL3 mutation affects information processing in neuronal networks by altering network architecture and synchrony PMID: 19406211

    显示更多

    收起更多

  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Cell junction, synapse. Note=Detected at both glutamatergic and GABAergic synapses.
  • 蛋白家族：
    Type-B carboxylesterase/lipase family
  • 组织特异性：
    Brain and arteries (at protein level). Detected in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis. Expressed in olfactory bulb and olfactory epithelium. Found in olfactory ensheathing glia but not in olfactory neurons, and in develo
  • 数据库链接：

    KEGG: mmu:245537

    STRING: 10090.ENSMUSP00000066304

    UniGene: Mm.121508