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  4. Recombinant Mouse Neurogenin-1 (Neurog1)

Recombinant Mouse Neurogenin-1 (Neurog1)

  • 中文名称：
    小鼠Neurog1重组蛋白
  • 货号：
    CSB-YP015730MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Neurog1重组蛋白
  • 货号：
    CSB-EP015730MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Neurog1重组蛋白
  • 货号：
    CSB-EP015730MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Neurog1重组蛋白
  • 货号：
    CSB-BP015730MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Neurog1重组蛋白
  • 货号：
    CSB-MP015730MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Neurog1; Ath4c; Neurod3; Ngn; Ngn1Neurogenin-1; NGN-1; Helix-loop-helix protein mATH-4C; mATH4C; Neurogenic basic-helix-loop-helix protein; Neurogenic differentiation factor 3; NeuroD3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-244
  • 氨基酸序列
    MPAPLETCIS DLDCSSSNSS SDLSSFLTDE EDCARLQPLA STSGLSVPAR RSAPALSGAS NVPGAQDEEQ ERRRRRGRAR VRSEALLHSL RRSRRVKAND RERNRMHNLN AALDALRSVL PSFPDDTKLT KIETLRFAYN YIWALAETLR LADQGLPGGS ARERLLPPQC VPCLPGPPSP ASDTESWGSG AAASPCATVA SPLSDPSSPS ASEDFTYGPG DPLFSFPGLP KDLLHTTPCF IPYH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

Could you please provide sequence, availability and tag information for all available sizes of CSB-BP015730MO?
Have you received any customer feedback about the activity of these proteins?
Please confirm also that Tag Removal Service is available for them.
Thank you !

A:
Thanks for your inquiry.
Recombinant Mouse Neurogenin-1(Neurog1)
CSB-BP015730MO >> Baculovirus
You can see the information of sequence and tag information on the website in details.
Sorry, we haven't received any feedback about the activity from other customers, and also haven't tested the activity, we can't 100% guarantee its activity.However, as what we provide is the full length protein and we adpot affinity chromatography purification, it's purified under very mild condition, it should be active, we recommend you to purchase a small size to have a try.
Sorry, as we haven't removed the tag of this protein before, in theory, tag removal service is available.
Please remark your requirement for tag removal if you need the untagged protein or tag removal when placing the order. We can try enzyme digestion, but we can't guarantee 100% successfully.The overall success rate of enzyme digestion data analysis is 75%-86%.
* Not all protein tags can be removed as some proteins will be very unstable after tag removal.
* If we succeed in removing the tag, we will charge for extra cost.
* If we fail in removing the tag, we will provide the fusion protein if you can accept and won’t charge for any extra cost, and remark this information in datasheet as follows
“Note: The laboratory determined that the Tag on your protein could not be removed with standard laboratory procedures. Your protein is being supplied with the Tag intact.”
* Generally, the delivery time will be extended for 3 days.

靶点详情

  • 功能：
    Acts as a transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis.
  • 基因功能参考文献：
    1. CTCF plays an essential role in otic neurogenesis by modulating histone modification in the Neurog1 locus. PMID: 30008200
    2. NEUROG1 promotes proliferation and neuronal differentiation in otic progenitors via regulation of CDK2 expression. PMID: 29033307
    3. Ngn1 binds to the promoter of a brain-enriched microRNA, miR-9, and activates its expression during neurogenesis. PMID: 26271009
    4. Neurog1-CreER GIFM reveals a correlation between the timing of Neurog1 expression and the spatial organization of GABAergic neurons in the cerebellar cortex. PMID: 25592069
    5. Neurog1 can partially substitute for Atoh1 function in hair cell differentiation and maintenance during organ of Corti development. PMID: 26209643
    6. Neurog1 and Neurog2 have unique and redundant functions in the piriform cortex, controlling the timing of differentiation of neurons PMID: 24403153
    7. ESC expressing Neurogenin1 begin to withdraw from cycle and form precursors that differentiate exclusively into neurons. PMID: 23288605
    8. Netrin-1 serves as an attractive guidance cue for Neurog1-induced cells and that this effect is likely mediated by DCC receptor expressed in the growth cones of neurons. PMID: 22512716
    9. Neurog1/2 together coordinate development of the olfactory system, which depends on tissue interactions between the olfactory bulb and epithelium. PMID: 22906231
    10. A noncoding RNA transcribed from an enhancer element positively regulates transcription at the Neurog1 locus. PMID: 23027973
    11. the formation of cranial ganglia along the anterior-posterior axis is dependent on the dynamic spatiotemporal activities of Neurog1 and/or Neurog2 in both neural crest and epibranchial placode. PMID: 22102600
    12. Expression of Neurog1 instead of Atoh1 can partially rescue organ of Corti cell survival. PMID: 22292060
    13. Neural zinc finger (NZF)-3 works downstream of Neurog1 and plays a crucial role together with Neurog1 in neuronal differentiation. PMID: 21540077
    14. Our data suggest functional Neurog1-Pax6 cross-talk in the activation of downstream targets in early cerebellar development PMID: 21382351
    15. This study provides the first overview of the spatiotemporal fate map of Neurogenin1 lineages in the CNS. PMID: 21452201
    16. Neurog1 expression in the ventral neural tube is mediated by a distinct enhancer and preferentially marks ventral interneuron lineages. PMID: 20171205
    17. Ngn1 is a component of the bHLH factor code regulating cell type specification in the cerebellar cortex. PMID: 19924827
    18. genetic analysis has identified Ngn1 as a gene required in basal progenitors to activate an OSN differentiation program, and force dividing progenitors out of the cell exit. PMID: 11934853
    19. neurogenin1 regulates neuroD2 expression during mouse brain development PMID: 14697366
    20. Sequential phases of cortical specification involve ngn1-dependent and -independent pathways. PMID: 15229646
    21. regulatory elements of ngn1 appear to be conserved among vertebrates, with certain differences being incorporated in the utilisation of these enhancers, for the acquisition of more advanced features in amniotes PMID: 15496438
    22. Ngn1 antagonizes differentiation of dI3 interneuron subtype by repressing dI3 progenitor development PMID: 15930101
    23. Neurog 1 null mice show a truncation of clonal expansion of hair cell precursors through temporally altered terminal mitosis PMID: 16145671
    24. Helt determines GABAergic over glutamatergic fate, by repressing Ngn genes; Helt and Ngns are commonly used in the mesencephalon for determination of the GABAergic versus glutamatergic transmitter phenotype. PMID: 17611227
    25. Ngn1, as well as promoting neurogenesis, maintains an uncommitted progenitor cell population through Notch-mediated lateral inhibition, and Math1 irreversibly commits these progenitors to a hair-cell fate. PMID: 18039969
    26. histone modifiers and chromatin remodelers are pivotal in the activation of the ngn1 gene in RA induced differentiation of P19 cells. PMID: 19308998

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Expression restricted to the embryonic nervous system.
  • 数据库链接：

    KEGG: mmu:18014

    STRING: 10090.ENSMUSP00000050484

    UniGene: Mm.266665