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  4. Recombinant Mouse Neurofilament light polypeptide (Nefl)

Recombinant Mouse Neurofilament light polypeptide (Nefl)

In Stock
  • 货号：
    CSB-YP015688MO
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Nefl; Nf68; NflNeurofilament light polypeptide; NF-L; 68 kDa neurofilament protein; Neurofilament triplet L protein
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    Yeast
  • 分子量：
    63.4kDa
  • 表达区域：
    2-543aa
  • 氨基酸序列
    SSFGYDPYFSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEIAFLKKVHEEEIAELQAQIQYAQISVEMDVSSKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRSTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYSGLQSSSYLMSARSFPAYYTSHVQEEQTEVEETIEATKAEEAKDEPPSEGEAEEEEKEKEEGEEEEGAEEEEAAKDESEDTKEEEEGGEGEEEDTKESEEEEKKEESAGEEQVAKKKD
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The gene fragment corresponding to the 2-543aa of the mouse Nefl protein was synthesized, with appropriate restriction sites suitable for in-frame cloning into an expression vector, with N-terminal 6xHis tag. The yeast was transfected with the expression vector, and the clone was expressed upon certain induction. After the induced cell centrifugation, the recombinant protein was purified from the cell extract and presented as N-terminal 6xHis-tagged fusion. This recombinant mouse Nefl protein's purity is greater than 90% assayed by SDS-PAGE. The Nefl protein ran to a band of about 50-66 kDa molecular weight on the gel, indicating a glycosylated form of the protein.

    Neurofilament light chain protein (NEFL), a cytoskeleton protein of neurons, can reflect the neuronal injury when appearing in biofluids. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. Researchers revealed that they are likely to play a role in intracellular transport to axons and dendrites. NEFL aberrantly expression was identified the in a sample of Iraqi multiple sclerosis patients. Additionally, NEFL participates the regulation of gastric carcinogenesis. NEFL gene mutation is common yet inefficient in neuroblastoma tumours. A properly assembled filamentous network is critical for neuronal function and abnormal expression of NEFL could be a potential mechanism of Schizophrenia, which is a severe neurodevelopmental disease with unknown pathogenic mechanisms characterized with impaired cognitive function.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.
  • 基因功能参考文献：
    1. Study found that NFL protein levels are at least doubled in pmn mutant motoneurons and that NFL depletion rescues defective axon growth in cultured motoneurons and prolongs survival of pmn mutant mice. This effect was found both in Nefl+/-;pmn motoneurons in which elevated NF expression was brought back to wild-type control levels and in Nefl-/-;pmn motoneurons in which axonal neurofilament was completely lost. PMID: 27021905
    2. It may have a role in protecting neurites from dystrophy and in regulating cellular pathways related to the generation of Ab plaques. PMID: 26344875
    3. Nefl(N98S/+) mice had a noticeable tremor, and most animals showed a hindlimb clasping similar to human Charcot-Marie-Tooth Type 2E phenotype. PMID: 25552649
    4. Mice lacking NF-Lr ecapitulated the delayed synapse elimination phenotype observed in micelacking Nfasc155. PMID: 25232125
    5. The finding of this study suggested that a lack of NFL protein alters the expression of cytoskeletal proteins and disrupts other NF subunits, causing intracellular aggregation but not gross structural changes in cortical neurons or cytoarchitecture. PMID: 23172043
    6. Neurofilament light chain (NFL) and neuronal intermediate filament protein alpha-internexin accumulate in axon swellings in the spinal white matter in a superoxide dismutase (SOD)-1 mouse model. PMID: 22609817
    7. Data suggest that tetrahydropapaveroline (an endogenous catechol) causes oxidative stress resulting in astrocyte/neuronal cell death via generation of reactive oxygen species and modification/aggregation of NF-L (as in neurodegenerative diseases). PMID: 23228886
    8. Data show that mitochondria essentially stopped moving in neurons expressing neurofilament protein (NFL) mutants, probably a consequence of cytoskeletal disruption. PMID: 22155564
    9. NEFL transgenic mice exhibited extended duration of the hindlimb clasping response and gait anomalies, as well as sensorimotor deficits in stationary beam and suspended bar tests PMID: 21168446
    10. Neuropathic effects of overexpressing NF-L can occur at the level of transgene RNA and are mediated by sequences in the NF-L 3' UTR PMID: 12196589
    11. nNOS inhibitor, AR-R17477AR, prevents the loss of NF68 immunoreactivity induced by methamphetamine in the mouse striatum PMID: 12675928
    12. The 3' untranslated region of light neurofilament (NF-L) transcript enhances the reactivity of its own translated product and leads to loss of solubility and aggregation of NF-L protein and to coaggregation of mutant superoxide dismutase 1 (SOD1) protein PMID: 15028764
    13. copper-mediated NF-L modification may be closely related to oxidative reactions which play a critical role in neurodegenerative diseases PMID: 15388232
    14. We observed three overlapping phases in NF-L transgenic mice, including transient aggregate formation, reactive microgliosis, and progressive motor neuron loss. PMID: 15920739
    15. p190RhoGEF is involved in aggregation of NF-L protein and support a working hypothesis that aggregation of p190RhoGEF and NF-L is an upstream event triggering neurotoxicity in motor neuron disease. PMID: 16236762
    16. Mouse lactotrophs, gonadotrophs, thyrotrophs and somatotrophs express NF68 in a sexually dimorphic manner. Mouse pituitary cells from the proopiomelanocortin lineage nearly completely lack NF68 immunoreactivity. PMID: 16940710
    17. Alpha-internexin coassembles with all 3 neurofilament proteins into a single network of filaments in quadruple-transfected cells. PMID: 17005864
    18. NFL gene deficiency could retard MSCs proliferation and neuronal generation, even though the capability of neuronal lineage differentiation of MSCs may not be deterred. PMID: 17395374
    19. Disruption of neurofilament network with aggregation of NFL is a common triggering event of motor neuron degeneration in Charcot-Marie-Tooth disease. PMID: 17881652
    20. Results suggest a preferential participation of P/Q-type Ca(2+) channels and hence alpha 1A subunits, in regulating spontaneous Ca(2+) transients in anterior pituitary cells under conditions where the proportion of NF68-expressing cells is high. PMID: 17927665
    21. Results suggests that acrolein-mediated NF-L aggregation might be closely related to oxidative reactions, thus these reactions may play a critical role in neurodegenerative diseases. PMID: 18823586
    22. LANP directly regulates expression of the neurofilament light chain, an important neuron-specific cytoskeletal gene, by binding to the promoter of this gene and modulating histone acetylation levels PMID: 19136565
    23. These results support that TDP-43 is involved in neurofilament mRNA metabolism and transport, and provide insight into the pathogenesis of motor neuron death in ALS in which NFL mRNA levels are selectively suppressed. PMID: 19619516

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  • 亚细胞定位：
    Cell projection, axon. Cytoplasm, cytoskeleton.
  • 蛋白家族：
    Intermediate filament family
  • 组织特异性：
    Expressed in the sciatic nerve (at protein level).
  • 数据库链接：

    KEGG: mmu:18039

    STRING: 10090.ENSMUSP00000022639

    UniGene: Mm.1956