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  4. Recombinant Mouse NF-kappa-B inhibitor zeta (Nfkbiz)

Recombinant Mouse NF-kappa-B inhibitor zeta (Nfkbiz)

  • 中文名称：
    小鼠Nfkbiz重组蛋白
  • 货号：
    CSB-YP875369MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Nfkbiz重组蛋白
  • 货号：
    CSB-EP875369MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Nfkbiz重组蛋白
  • 货号：
    CSB-EP875369MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Nfkbiz重组蛋白
  • 货号：
    CSB-BP875369MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Nfkbiz重组蛋白
  • 货号：
    CSB-MP875369MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Nfkbiz
  • Uniprot No.：
  • 别名：
    Nfkbiz; Inap; Mail; NF-kappa-B inhibitor zeta; I-kappa-B-zeta; IkB-zeta; IkappaBzeta; IL-1 inducible nuclear ankyrin-repeat protein; INAP; Molecule possessing ankyrin repeats induced by lipopolysaccharide; MAIL
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-728
  • 氨基酸序列
    MIVDKLLDDS RGGEGLLDAA GDCGLMTSPL NLAYFYGASP PSAPGAGDTG YLSAVPSAPG SPGSDSSDFS STSSVSSCGA VESRPRGGAR AERPQVEPHM GVGRQQRGPF QGVRVKNSVK ELLLHIRSNK QKASGQPVDE FKTQSVNIEQ LTDLKSAVSA VGKRKGPDPL SDGPVCKRPA LLPSHFVTSP QTPTPGESME DVRHSESKLD SSAALLQNII NIKNECNPVS LNTVQVSWMS PTVPQNSPRD QCQDFHGGQA FSPPQKYQPF QVSGSPQMMD QASMYQYSPQ TQNMQQPPPL PPQQQHQQNY PHNSPLQFSP YSRMSQSPKY DSNLFDTHEP QFCTGQSFVS LLTGPGEPES LAVPVPAPTS IPPQTETQLQ TFSLMPSNAC EAVVGVHDVG SHSLGTSLSL QNIMGSPMNT TQLGKSFFQW QVEQEESKLA NIPQDQFLAR DGDGDTFLHI AVAQGRRALS YVLARKMNAL HMLDIKEHNG QSAFQVAVAA NQHLIVQDLV NLGAQVNTTD CWGRTPLHVC AEKGHSQVLQ AIQKGAVRSN QFVDLEATNY DGLTPLHCAV VAHNAVVHEL QRNRQSHSPE VQDLLLRNKS LVDTIKCLIQ MGAAVEAKDR KSGRTALHLA AEEANLELIR LFLELPSCLS FVNAKAYNGN TALHVAASLQ YRVTQLDAVR LLMRKGADPS TRNLENEQPV HLVPDGPVGE QIRRILKGKS IQQRAPPY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in regulation of NF-kappa-B transcription factor complexes. Inhibits NF-kappa-B activity without affecting its nuclear translocation upon stimulation. Inhibits DNA-binding of RELA and NFKB1/p50, and of the NF-kappa-B p65-p50 heterodimer and the NF-kappa-B p50-p50 homodimer. Seems also to activate NF-kappa-B-mediated transcription. In vitro, upon association with NFKB1/p50 has transcriptional activation activity and, together with NFKB1/p50 and RELA, is recruited to LCN2 promoters. Promotes transcription of LCN2 and DEFB4. Is recruited to IL-6 promoters and activates IL-6 but decreases TNF-alpha production in response to LPS. Seems to be involved in the induction of inflammatory genes activated through TLR/IL-1 receptor signaling. May promote apoptosis. Involved in the induction of T helper 17 cells (Th17) differentiation upon recognition of antigen by T cell antigen receptor (TCR).
  • 基因功能参考文献：
    1. results uncover an important role for IkappaBzeta in IL-36 signaling and validate IkappaBzeta as an attractive target for psoriasis therapy. PMID: 30224457
    2. electrophilic properties of itaconate and derivatives regulate the IkappaBzeta-ATF3 inflammatory axis; results demonstrate that targeting the DI-IkappaBzeta regulatory axis could be an important new strategy for the treatment of IL-17-IkappaBzeta-mediated autoimmune diseases PMID: 29670287
    3. In cultured macrophages, LPS-induced expression of monocyte chemoattractant protein (MCP)-1 was suppressed by NaN3 through inhibition of STAT1 and IkappaBzeta activities. PMID: 28391993
    4. Data show that I-kappa-B-zeta (IkappaBzeta) regulates macrophage interleukin-10 (IL_10) expression by directly activated the Il-10 promoter. PMID: 27129283
    5. A possible important role of Nfkbiz in modulating the progression of inflammatory diseases in which IFN-gamma and IL-1 are abundant. PMID: 25876660
    6. Nfkbiz-/- keratinocytes were hypoproliferative. In skin from Nfkbiz-/- mice, the expression of the keratinocyte differentiation markers K10 and filaggrin were reduced, although that of K14 was unchanged. PMID: 26563030
    7. These data reveal a previously unappreciated role for IkappaB-zeta in IFN-gamma production in T cells and the immunoregulatory function of T regulatory cells. PMID: 26019294
    8. The pyrimidine-rich IkappaBzeta-responsive site plays an essential role in productive interaction of IkappaBzeta with the p50-DNA complex. PMID: 24948478
    9. IkappaB-zeta regulates TLR-mediated CSR by inducing AID PMID: 25124037
    10. The IkappaB-zeta-Akirin2-BAF60 complex physically links the NF-kappaB and SWI/SNF complexes in innate immune cell activation. PMID: 25107474
    11. The IkappaB-zeta-deficient B cells exhibited impaired proliferation and enhanced up-regulation of CD86 following stimulation of TLR9, but not the BCR, indicating critical roles for IkappaB-zeta in TLR signaling in B cells. PMID: 23728777
    12. IkappaBzeta-deficient mice showed significantly impaired CCL2 secretion. PMID: 23547114
    13. Data indicate that expression of IkappaB-zeta in the lacrimal gands requires STAT3. PMID: 23453632
    14. extracellular signal regulated kinases modulate IL-12 expression by regulating IkappaB-zeta-dependent binding of NF-kappaB transcription factors to IL-12b gene promoter PMID: 22131231
    15. IkappaBzeta is essential for natural killer cell activation in response to IL-12 and IL-18 and antiviral host defense responses. PMID: 20876105
    16. In cooperation with RORgammat and RORalpha, IkappaBzeta enhances Il17a expression by binding directly to the regulatory region of the Il17a gene PMID: 20383124
    17. Bacterial lipopolysaccharide induces mRNA expression of an IkappaB MAIL through toll-like receptor 4. PMID: 12069074
    18. These results indicate the existence of another signal essential for I kappa B-zeta induction, which is specifically mediated by the Toll/interleukin-1 receptor (TIR) domain-mediated signaling pathway. PMID: 12565889
    19. LPS induces MAIL-L predominantly in B-lymphocytes and macrophages. PMID: 12703554
    20. IkappaBzeta is indispensable for the expression of a subset of genes activated in TLR/IL-1R signalling pathways PMID: 15241416
    21. MAIL is an essential molecule for homeostatic regulation of skin immunity PMID: 15491998
    22. IkappaB-zeta induction requires both NF-kappaB activation and stimulus-specific stabilization of its mRNA PMID: 15522867
    23. Data indicate that MAIL expression is strongly upregulated by NF-kappaB, and it is controlled, at least in part, by an autoregulation mechanism. PMID: 15527973
    24. IkappaB-zeta harbors latent transcriptional activation activity which is expressed upon interaction with the NF-kappaB p50 subunit PMID: 15618216
    25. The constitutive expression of MAIL in keratinocytes is controlled, at least in part, by NF-kappaB and that there may be LPS-specific repressive mechanisms that inhibit MAIL induction. PMID: 17409644
    26. The recruitment of NF-kappaB, RNA polymerase, and the TATA-binding protein is MyD88-dependent only at secondary response genes. PMID: 18319258
    27. IkappaB-zeta is expressed independently of inflammatory stimuli to specifically investigate the molecular basis underlying IkappaB-zeta-mediated gene activation PMID: 18824552
    28. MAIL localizes in the nucleus, and accelerates IL-6 production by LPS stimulation in mouse fibroblasts. PMID: 11086164

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Expressed in kidney, liver, lung and heart. Expressed at very low levels in skeletal muscle, spleen and brain.
  • 数据库链接：

    KEGG: mmu:80859

    STRING: 10090.ENSMUSP00000041173

    UniGene: Mm.247272