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  4. Recombinant Mouse Myoblast determination protein 1 (Myod1)

Recombinant Mouse Myoblast determination protein 1 (Myod1)

  • 中文名称：
    小鼠Myod1重组蛋白
  • 货号：
    CSB-YP015358MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Myod1重组蛋白
  • 货号：
    CSB-EP015358MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Myod1重组蛋白
  • 货号：
    CSB-EP015358MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Myod1重组蛋白
  • 货号：
    CSB-BP015358MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Myod1重组蛋白
  • 货号：
    CSB-MP015358MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Myod1
  • Uniprot No.：
  • 别名：
    Myod1; Myod; Myoblast determination protein 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-318
  • 氨基酸序列
    MELLSPPLRD IDLTGPDGSL CSFETADDFY DDPCFDSPDL RFFEDLDPRL VHMGALLKPE EHAHFPTAVH PGPGAREDEH VRAPSGHHQA GRCLLWACKA CKRKTTNADR RKAATMRERR RLSKVNEAFE TLKRCTSSNP NQRLPKVEIL RNAIRYIEGL QALLRDQDAA PPGAAAFYAP GPLPPGRGSE HYSGDSDASS PRSNCSDGMM DYSGPPSGPR RQNGYDTAYY SEAARESRPG KSAAVSSLDC LSSIVERIST DSPAAPALLL ADAPPESPPG PPEGASLSDT EQGTQTPSPD AAPQCPAGSN PNAIYQVL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
  • 基因功能参考文献：
    1. Linc-RAM regulates expression of myogenic genes by directly binding MyoD, which in turn promotes the assembly of the MyoD-Baf60c-Brg1 complex on the regulatory elements of target genes. PMID: 28091529
    2. ACL regulates the net amount of acetyl groups available, leading to alterations in acetylation of H3(K9/14) and H3(K27) at the MYOD locus, thus increasing MYOD expression. PMID: 29241530
    3. Studies indicate MyoD displays function to regulate determination of skeletal muscle progenitors [Review]. PMID: 29097153
    4. Muscle regulatory transcription factor MyoD regulates the expression of the pro-apoptotic Bcl2 family member PUMA by binding to the promoter region of PUMA. The increase in MyoD binding to the PUMA promoter as a consequence of culture in differentiation media (DM) is diminished in myoblasts silenced for MyoD expression. In myoblasts silenced for MyoD expression, p53 binding to the PUMA promoter is diminished in response PMID: 28918507
    5. our results indicate that HDAC11 would suppress myoblast differentiation via regulation of MyoD-dependent transcription. These findings suggest that HDAC11 is a novel critical target for controlling myoblast differentiation. PMID: 28927261
    6. The data showed that Mettl3 is required for MyoD mRNA expression in proliferative myoblasts. PMID: 28878038
    7. Gm7325, as a novel MyoD-target gene, is specifically induced in activated satellite cells, and may have an important role in skeletal myogenesis. PMID: 28637957
    8. The ELF-EMFs did not affect C2C12 myoblast viability or proliferation rate. Conversely, at ELF-EMF intensity in the mT range, the myogenic process was accelerated, through increased expression of MyoD, myogenin, and connexin 43 PMID: 28607928
    9. In this study, we identified ubiquitin-specific protease 4 (USP4), one of deubiquitinating enzymes, as a suppressor of MRFs by demonstrating that a knockdown of USP4 enhances myogenesis by controlling MyoD and the level of myogenesis marker proteins in C2C12 cells... we propose that USP4 is a key player in myogenic differentiation; it controls myogenic regulatory factors in a catalytic-independent manner PMID: 28336234
    10. MyoD regulates the oxidative metabolic capacity of adult skeletal muscle;ChIP-seq analysis identified MyoD binding on the PGC-1b, but not PGC-1a, gene locus;MyoD cooperates with alternative NF-kappaB to regulate PGC-1b transcription; MyoD and RelB co-occupy many other genes involved in aerobic respiration PMID: 27705798
    11. LSD1 is required for the timely expression of MyoD in limb buds. PMID: 28228264
    12. bn1 expression is induced during myoblast differentiation, in a p38 MAP kinase- and MyoD- dependent manner. RNAi-mediated depletion of drebrin, or treatment with a chemical drebrin inhibitor, resulted in a similar phenotype in myoblasts: defective differentiation, with low levels of early and late differentiation markers and inefficient production of myofibers. PMID: 28865032
    13. Data show that MyoD and myogenin associate with distinct chromatin states. PMID: 28981706
    14. Results indicate the importance of integrating histone modifications and MyoD chromatin binding for coordinated gene activation and repression during myogenic differentiation. PMID: 28609469
    15. Data suggest Tle3 plays role in regulation of MyoD1 function during myogenesis; up-regulation of Tle3 expression suppresses myogenesis; conversely, down-regulation of Tle3 expression promotes myogenesis/cell proliferation; Tle3 interferes with MyoD1 by disrupting association of basic helix-loop-helix domain of MyoD with E proteins. (Tle3 = transducin-like enhancer of split 3; MyoD1 = myogenic differentiation protein 1) PMID: 28607151
    16. significant co-localization of binding sites for MyoD and Six proteins on over a thousand mouse genomic DNA regions, were found. PMID: 27302134
    17. Through mutational analysis, we derive an optimally active phospho-mutant form of MyoD that has a dramatically enhanced ability to drive myogenic reprogramming in vivo. Mechanistically, this is achieved through increased protein stability and enhanced chromatin association. Therefore, multi-site phospho-regulation of class II bHLH proteins is conserved across cell lineages and germ layers, and manipulation of phosphorylat PMID: 27823936
    18. results suggest that the miR-29a-Tet1 pathway upregulates MyoD expression and conversely downregulates Cdk6 expression PMID: 27349543
    19. Data show that myoblast determination protein 1 (MyoD) acts partially through microRNA miR-133 to suppress brown adipocyte cell fate. PMID: 28117277
    20. This evidence demonstrates that TBP2 does not replace TBP during muscle differentiation, as previously proposed, with limiting amounts of TFIID-TBP being required to promote muscle-specific gene expression PMID: 26880551
    21. established a model in which FHL3 promotes the expression of MyHC 2a through CREB-mediated transcription and inhibits the expression of MyHC 1/slow by inhibiting MyoD transcription activity during myogenesis PMID: 26499038
    22. The mechanism of bakuchiol-induced myogenesis is described. PMID: 26902638
    23. The large TBP polyQ repeat decreases the association of MyoD with TBP and DNA promoters and causes muscle degeneration in spinocerebellar ataxia 17 transgenic mice. PMID: 26387956
    24. results reveal a role for Six4 during adult muscle regeneration and suggest a widespread mechanism of cooperation between Six4 and MyoD. PMID: 26229056
    25. redirecting MyoD binding from MyoD private sites to NeuroD2 private sites PMID: 25801030
    26. Jmjd2C increases MyoD transcriptional activity to facilitate skeletal muscle differentiation by increasing MyoD stability through inhibiting G9a-dependent MyoD degradation. PMID: 26149774
    27. MyoD affects chromatin looping at CCCTC-binding factor-binding sites represents the first evidence that a differentiation factor regulates chromatin-loop dynamics PMID: 25002401
    28. MyoD differentially promotes activated and repressed chromatin structures at myogenic genes early after the onset of skeletal muscle differentiation in the developing mouse embryo. PMID: 25329411
    29. Duxbl overexpression in C2C12 cells inhibited myogenic differentiation by decreasing MyoD downstream gene expressions, including M-cadherin, MyoG, p21 and cyclin D3 but not MyoD itself. PMID: 25130140
    30. KAP1 as a previously unappreciated interpreter of cell signaling, which modulates the ability of MyoD to drive myogenesis. PMID: 25737281
    31. KDM4B histone demethylase regulates expression of myogenic regulators such as MyoD and thereby controls myogenic differentiation of C2C12 myoblast cells. PMID: 25534856
    32. MUNC is not a classic cis-acting enhancer RNA (e-RNA) acting exclusively by stimulating the neighboring MyoD gene PMID: 25403490
    33. Kazinol-P promotes myogenic differentiation through activation of p38MAPK and MyoD transcription activities PMID: 25482443
    34. Nrf2 deficiency promotes apoptosis and impairs PAX7/MyoD expression in aging skeletal muscle cells. PMID: 24613379
    35. we have demonstrated that Kbtbd5 is the direct downstream target gene of the Myod family and regulates myogenic differentiation. PMID: 24361185
    36. observations suggest that MyoD and pRb work together cooperatively at the level of the intronic enhancer of Fra-1 during terminal cell cycle arrest PMID: 25006242
    37. MyoD was required for the induction of FGF21 gene transcription by mitochondrial dysfunction. PMID: 25055037
    38. Cross-repressive interactions between MyoD- and PPARgamma-induced differentiation programs ensure discrete cell-fate decisions. PMID: 24703953
    39. fused the MyoD domain to Mef2c, Gata4, Hand2, and Tbx5 and transduced these genes in various combinations into mouse non-cardiac fibroblasts PMID: 23794713
    40. ABL-MyoD signaling coordinates DNA repair and transcription in myoblasts. PMID: 24056763
    41. 31-kDa caspase-generated cleavage product of p130Cas inhibited MyoD-induced transcriptional activation of muscle-specific genes. PMID: 24472550
    42. We show for the first time that p300 is directly involved in the early regulation of Myod1 enhancer, and provide molecular insights into how p300 HAT activity and histone acetylation are related to enhancer activation. PMID: 23928680
    43. the NFAT member NFATc2 cooperates with the basic helix-loop-helix transcription factor MyoD to induce the expression of a specific myosin heavy chain isoform, the neonatal one, during embryogenesis. PMID: 24301466
    44. Although expression of MyoD in a proliferating tumor is insufficient to prevent tumor progression, its expression in the cerebellum hinders medulloblastoma genesis. PMID: 24092238
    45. Extensive MyoD and MyoG occupancy in the extragenic regions. RNA synthesis at MyoD+/MyoG+ extragenic enhancer sites. PMID: 23993744
    46. The expression levels of myoD, miR-29a, miR-29b, and miR-29c were increased in L6 myoblast differentiated to myotube. PMID: 24074615
    47. Direct reprogramming of fibroblasts to myocytes via bacterial injection of MyoD protein. PMID: 23438194
    48. Myod and H19-Igf2 locus interactions are required for diaphragm formation. PMID: 23406902
    49. In addition to regulating MyoD expression, Hh signaling regulates MyoD transcriptional activity, and MyoD activates Hh signaling PMID: 23266826
    50. MyoD(-/-) myoblasts impart beneficial effects after transplantation into an infarcted hear PMID: 22848585

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  • 亚细胞定位：
    Nucleus.
  • 数据库链接：

    KEGG: mmu:17927

    STRING: 10090.ENSMUSP00000072330

    UniGene: Mm.1526