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  4. Recombinant Mouse Mothers against decapentaplegic homolog 7 (Smad7)

Recombinant Mouse Mothers against decapentaplegic homolog 7 (Smad7)

  • 中文名称：
    小鼠Smad7重组蛋白
  • 货号：
    CSB-YP021793MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Smad7重组蛋白
  • 货号：
    CSB-EP021793MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Smad7重组蛋白
  • 货号：
    CSB-EP021793MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Smad7重组蛋白
  • 货号：
    CSB-BP021793MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Smad7重组蛋白
  • 货号：
    CSB-MP021793MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Smad7; Madh7; Madh8; Mothers against decapentaplegic homolog 7; MAD homolog 7; Mothers against DPP homolog 7; Mothers against decapentaplegic homolog 8; MAD homolog 8; Mothers against DPP homolog 8; SMAD family member 7; SMAD 7; Smad7
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-426
  • 氨基酸序列
    MFRTKRSALV RRLWRSRAPG GEDEEEGVGG GGGGGELRGE GATDGRAYGA GGGGAGRAGC CLGKAVRGAK GHHHPHPPTS GAGAAGGAEA DLKALTHSVL KKLKERQLEL LLQAVESRGG TRTACLLLPG RLDCRLGPGA PASAQPAQPP SSYSLPLLLC KVFRWPDLRH SSEVKRLCCC ESYGKINPEL VCCNPHHLSR LCELESPPPP YSRYPMDFLK PTAGCPDAVP SSAETGGTNY LAPGGLSDSQ LLLEPGDRSH WCVVAYWEEK TRVGRLYCVQ EPSLDIFYDL PQGNGFCLGQ LNSDNKSQLV QKVRSKIGCG IQLTREVDGV WVYNRSSYPI FIKSATLDNP DSRTLLVHKV FPGFSIKAFD YEKAYSLQRP NDHEFMQQPW TGFTVQISFV KGWGQCYTRQ FISSCPCWLE VIFNSR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
  • 基因功能参考文献：
    1. We confirmed that Smad7 was a direct target of miR-21. miR-21 knockout resulted in increased expression of Smad7 and impaired phosphorylation of the Smad2/3 complex PMID: 29154818
    2. Eplerenone inhibited atrial fibrosis in MHC-TGFcys33ser transgenic mice. The underlying mechanism may involve increased protein expression of Smad7, which enhances the inhibitory feedback regulation of TGF-beta1/Smad signaling. PMID: 28571007
    3. Smad7 enables STAT3 activation and promotes pluripotency independent of TGF-beta signaling PMID: 28874583
    4. lentiviral vector-mediated i.a. overexpression of Smad7 can ameliorate rheumatoid joint, implicating a pharmacological development of Smad7-based molecular strategy in collagen-induced arthritis PMID: 27731365
    5. Smad7 expression governs splenic DC subset differentiation. PMID: 28167776
    6. Our results indicate that miR-212-3p facilitates the activation of HSCs and TGF-beta pathway by targeting SMAD7, highlighting that it can be served as a novel biomarker or therapeutic target for liver fibrosis. PMID: 29307832
    7. a heterozygous deletion of SMAD7 results in an increased proliferation of Muller cell progenitors in the central retina at postnatal day 4, the time window when Muller cells differentiate in the mouse retina; this in turn results in a thickened retina and inner nuclear layer and a higher number of differentiated Muller cells in the more developed retina PMID: 28258388
    8. changes were associated with increased expression of the antifibrotic protein Smad7 and higher levels of sphingosine in Sphk2(-/-) unilateral ureteral obstruction kidneys PMID: 28807595
    9. Smad7 plays a protective role in acute kidney injury by preventing tubular epithelial cells from the G1 cell cycle arrest. PMID: 28566468
    10. study reports an alternately-spliced form of Smad7, Smad7Delta, that is induced by TGF-beta and CLIC4, is a dominant inhibitor of Smad7 and enhances TGF-beta signaling PMID: 27536941
    11. Pancreatic depletion of SMAD7 resulted in age-dependent increases in beta cell dysfunction with accelerated glucose intolerance, followed by overt diabetes. PMID: 28057752
    12. The inverse correlation between Smad7 and AhR expression helps to propagate inflammatory signals in the gut in Crohn's disease. PMID: 26818761
    13. results indicate that miR-181a-5p promotes 3T3-L1 preadipocyte differentiation and adipogenesis through regulating TGFbeta/Smad and Wnt signaling pathway by directly targeting Smad7 and Tcf7l2 PMID: 27742678
    14. microRNA-497 modulates breast cancer cell proliferation, invasion, and survival by targeting SMAD7. PMID: 27303812
    15. results indicate that miR-195a-5p inhibits MTEC1 proliferation, at least in part, via down-regulation of Smad7 PMID: 26837421
    16. Data indicate that, in irritable bowel disease, high Smad7 contributes to sustain detrimental immune responses and knockdown of this molecule can help attenuate the ongoing mucosal inflammation in patients with such disorders PMID: 26651974
    17. Hic-5 deficiency attenuates the activation of hepatic stellate cells and liver fibrosis though reducing the TGF-beta/Smad2 signaling by upregulation of Smad7 PMID: 26334580
    18. Data show that microRNA miR-21 was significantly upregulated by bone morphogenetic protein 9 (BMP9) during the osteogenesis the multilineage cells (MMCs) by suppressing Smad7 protein. PMID: 26460584
    19. Genetic disruption of Smad7 impairs skeletal muscle growth and regeneration PMID: 25854148
    20. The present study also identified for the first time, to the best of our knowledge, the promotion of osteogenic differentiation and mineralization by miR-21, by repressing the expression of Smad7. PMID: 25815684
    21. Overexpression of Smad7 in human HaCaT keratinocyte cells and mouse skin tissues elevated EGF receptor (EGFR) activity by impairing ligand-induced ubiquitination and degradation of activated receptor, which is induced by the E3 ubiquitin ligase c-Cbl. PMID: 26055326
    22. Smad7 signaling is repressed by the active form of vitamin D, 1,25-dihydroxyvitamin D3, which also activates ERK to inhibit the differentiation of an inflammatory T helper cell subset and suppress experimental autoimmune encephalomyelitis PMID: 25809484
    23. Study identified miR-367 as a negative regulator of Smad7 expression in pancreatic cancer cells by directly targeting its 3'-UTR. PMID: 25867271
    24. TGF-beta dependent modulation of neurogenesis in aged S7DeltaEx1 animals abrogates the cell-intrinsic hyperproliferative properties PMID: 24862634
    25. The results indicate that partial loss of Smad7 function in mice leads to compromised bone formation and enhanced bone resorption. Thus, Smad7 is acknowledged as a novel key regulator between osteogenesis and osteoclastogenesis. PMID: 24998669
    26. Smad7 enhances ATM activity by facilitating the interaction between ATM and Mre11-Rad50-Nbs1 complex in DNA double-strand break repair. PMID: 25063542
    27. Small interfering RNA for lncRNA-Smad7 did not alter the process of TGF-beta-induced epithelial-mesenchymal transition, phosphorylation of Smad2 or expression of the Smad7 gene PMID: 24863656
    28. Report role for Smad7 in sustaining colon tumorigenesis. PMID: 24556688
    29. Smad7 plays a crucial role in antagonizing epithelial-mesenchymal transition induced by TGFbeta signaling and is a Notch signaling target in limbal epithelial stem cells. PMID: 24791137
    30. The results demonstrate an essential and non-redundant role for Cbl-b in controlling TGFbetaR signaling by directly targeting SMAD7 for degradation during T cell responses in vitro and in vivo. PMID: 23709694
    31. SMAD7 promotes beta-cell proliferation by increasing CyclinD1 and CyclinD2, and by inducing nuclear exclusion of p27. PMID: 24639504
    32. Smad7 plays a protective role in Ang II-induced cardiac remodelling via mechanisms involving the Sp1-TGF-beta/Smad-NF-kappaB-miR-29 regulatory network. PMID: 23761400
    33. Smad7 plays a protective role in AngII-mediated cardiac remodeling via mechanisms involving the Sp1-TGF-beta/Smad3-NF.kappaB-miR-29 regulatory network. PMID: 23894614
    34. SMAD7 is expressed in granulosa cells, is subject to regulation by intraovarian growth factors from the transforming growth factor (TGF)beta superfamily, and is a negative regulator of TGFbeta. PMID: 23633623
    35. Smad7 may be required to mediate cell stress responses in the growth plate during development. PMID: 23994637
    36. Phosphorylation levels of Smad2 and Smad3 were slightly reduced in murine embryonic fibroblast (MEF) cells isolated from Smad7-deficient embryos compared with wild-type MEF cells. PMID: 23509008
    37. Smad7 suppresses hepatocellular carcinoma cell growth by inhibiting proliferation and G1 -S phase transition and inducing apoptosis through attenuation of NF-kappaB and TGFbeta signalling. PMID: 23625826
    38. Smad7 promotes ANG II-mediated renal fibrosis and inflammation by Sp1-TGF-beta1/Smad3-NF-kappaB signaling and loss of miR-29 PMID: 23301086
    39. The Smad7 plays an important role in normal mouse growth and provide a useful tool for analysing Smad7 functions in vivo. PMID: 22383537
    40. Smad7 is essential for eye development. PMID: 23426374
    41. Tbx1 acts upstream of Smad7 controlling vascular smooth muscle and extracellular matrix investment of the fourth arch artery. PMID: 23011393
    42. Smad7 mediates apoptosis induced by TGF-beta in mouse granulosa cells, suggesting that dysregulation of Smad7 could impair folliculogenesis. PMID: 22656308
    43. Candesartan inhibited TGF-beta1 signal-induced epithelial-to-mesenchymal transition (EMT) through attenuating matrix metalloproteinases (MMP-9), such effect was abolished by knocking-down Smad7. PMID: 22326534
    44. Loss of renal Smad7 is an underlying mechanism required for the promotion of TGF-b/Smad-mediated renal fibrosis and NF-kappaB-driven renal inflammation in the unilateral ureteral obstructive (UUO) nephropathy. PMID: 22330342
    45. Kidney-targeting Smad7 gene transfer inhibits renal TGF-beta/MAD homologue (SMAD) and nuclear factor kappaB (NF-kappaB) signalling pathways, and improves diabetic nephropathy in mice PMID: 22086159
    46. Data show that Smad7 overexpression increased colitis severity, but the mice nevertheless developed fewer tumors than nontransgenic mice. PMID: 22028324
    47. Epidermal Smad7 overexpression accelerated wound healing. PMID: 21944279
    48. CYLD regulates TGF-beta signaling function in T cells and the development of Tregs through deubiquitination of Smad7 PMID: 21931165
    49. In vivo evidence revealing that endogenous Smad7 plays an important role in liver function and alcohol-induced liver injury. PMID: 21386907
    50. Smad7 plays a protective role in diabetic renal injury. PMID: 20980457

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    Dwarfin/SMAD family
  • 组织特异性：
    Ubiquitous in various organs, with higher levels in brain and kidney.
  • 数据库链接：

    KEGG: mmu:17131

    STRING: 10090.ENSMUSP00000026999

    UniGene: Mm.34407