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  4. Recombinant Mouse Mast cell protease 4 (Mcpt4)

Recombinant Mouse Mast cell protease 4 (Mcpt4)

In Stock
  • 中文名称：
    小鼠Mcpt4重组蛋白
  • 货号：
    CSB-EP325157MO
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    Mcpt4
  • Uniprot No.：
  • 别名：
    Mast cell protease 4; Mcp 4; Mcpt4; MCPT4_MOUSE; MMCP 4; MMCP 4A; MMCP 4B; MMCP-4; MMCP4; MMCP4A; MMCP4B; MSMCP; Myonase; Serosal mast cell protease
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    29.1kDa
  • 表达区域：
    21-246aa
  • 氨基酸序列
    IIGGVESRPHSRPYMAHLEITTERGFTATCGGFLITRQFVMTAAHCSGREITVTLGAHDVSKTESTQQKIKVEKQIVHPKYNFYSNLHDIMLLKLQKKAKETPSVNVIPLPRPSDFIKPGKMCRAAGWGRTGVTEPTSDTLREVKLRIMDKEACKNYWHYDYNLQVCVGSPRKKRSAYKGDSGGPLLCAGVAHGIVSYGRGDAKPPAVFTRISSYVPWINRVIKGE
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The preparation of Recombinant Mouse Mcpt4 protein included 3 main steps: construct the expression vector, expression of protein of interest, and protein purification. Every step was performed under a strict QC system so that we got the premium protein. This Mcpt4 was expressed in E.coli at and fused with N-terminal 6xHis tag. According to SDS-PAGE, the purity turns out to be 90%+.

    Mcpt4 is the chymase mouse mast cell protease 4. MCPT4-deficient mice had worsened kidney function compared to wildtype mice. MCPT4 absence exacerbated pathologic neutrophil infiltration in the kidney and increased kidney myeloperoxidase expression, cell death and necrosis. Besides, studies revealed that MCPT4 Chymase Promote Renal Impairment after partial ureteral obstruction. Among the murine chymases, MCPT4 is most likely the functional counterpart of the unique human chymase because it has similar substrate specificity, tissue distribution, proteoglycan-binding properties, and ability to convert angiotensin (Ang) I into Ang II. A study found that mast cell can significantly contribute to the development of UPJ pathology. This action is at least partially mediated by released MCPT4 chymase.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

About Mcpt4 Recombinant Protein (CSB-EP325157MO), we have some questions:
1. What is the format of MCPT4 enzyme? Is it a MCPT4 precursor format, or a matured MCPT4 protein?
2. What are the prices for the different prices? Kindly provide the lead time.

A:
Very nice to receive your inquiry.
1. This is a matured MCPT4 protein.
2. Lead Time: 15-20 working days

靶点详情

  • 功能：
    Has chymotrypsin-like activity. Hydrolyzes the amide bonds of synthetic substrates having Tyr and Phe residues at the P1 position. Preferentially hydrolyzes the 'Tyr-4-|-Ile-5' bond of angiotensin I and the 'Phe-20-|-Ala-21' bond of amyloid beta-protein, and is less active towards the 'Phe-8-|-His-9' bond of angiotensin I and the 'Phe-4-|-Ala-5' and 'Tyr-10-|-Glu-11' bonds of amyloid beta-protein. Involved in thrombin regulation and fibronectin processing.
  • 基因功能参考文献：
    1. deletion of the gene encoding MMCP-4 (Mcpt4), markedly reduced late, but not early, infarct size by suppressing IGF-1 degradation and, consequently, diminished cardiac dysfunction and adverse structural remodeling PMID: 27274047
    2. study supports a role for mMCP-4 in the early inflammatory phases of the disease in a mouse model of MS. PMID: 27610007
    3. These results suggest that the inhibition of mMCP-4 reduces lesion spreading in the earlier phases of atherosclerosis development and can help stabilise the more advanced plaque PMID: 26976326
    4. Results suggest that mouse mast cell proteases 4, 5, and 6 are mediators of the critical role mast cells play in microdeformational wound therapy in the proliferative phase of healing. PMID: 24814421
    5. Data indicate that a second-degree burn injury can initiate an immediate novel zonal degranulation of mast cell throughout all skin layers and a disruption of the epidermal tight junctions dependent on the nonredundant presence of mMCP4 and mMCP5. PMID: 24523504
    6. these results support the conclusion that mast cells can contribute to the initial lung injury induced by bleomycin through release of the MCPT4 chymase. PMID: 24453258
    7. knock-outs demonstrate elevated airway reactivity and eosinophilia, and higher levels of serum immunoglobulin E PMID: 23235745
    8. Mast cell chymase degrades the alarmins heat shock protein 70, biglycan, HMGB1, and interleukin-33 (IL-33) and limits danger-induced inflammation. PMID: 24257755
    9. Mast cell released mMCP4 has anti-fibrotic potential in acutely induced obstructive nephropathy. PMID: 23515052
    10. Data from Mcp-4 (mast cell protease 4) knockout mice suggest Mcp-4 plays a pivotal role in the dynamic (in vivo and in vitro) conversion of systemic Big-endothelin-1 to endothelin-1 (1-31). PMID: 23596057
    11. MCs exert protective functions after trauma, at least in part via mMCP-4, by suppressing exacerbated inflammation via their proteases. PMID: 23193170
    12. The effects of interactions between mMCP-4 and TNF in vivo by analyzing the features of a classic model of polymicrobial sepsis, were assessed. PMID: 22901752
    13. mMCP-4 plays two different roles in the pathogenesis of experimental BP, by both activating MMP-9 and by cleaving BP180, leading to injury of the hemidesmosomes and extracellular matrix of the basement membrane zone. PMID: 21880713
    14. mMCP-4-deficient mice but not to mMCP-5-deficient mice revealing nonredundant actions for these two MC proteases in a model of innate inflammatory injury with remodeling. PMID: 21076070
    15. MCP-4 contributes locally to the aggravation of glomerulonephritis by mediating a variety of proinflammatory effects. PMID: 20530261
    16. mice deficient in mast cells (Kit(W-sh/W-sh) [Wsh]) or mast cell chymase (Mcpt4(-/-)) have significantly decreased basal small intestinal permeability compared with wild-type (WT) mice PMID: 20018751
    17. localization in skeletal muscle myofibrils PMID: 12204111
    18. Data describe a mouse strain with a targeted deletion in the gene for mast cell protease 4 (mMCP-4), in terms of tissue localization and functional properties. PMID: 12900518
    19. mast cell mMCP-4, -5, and -6 (chymase and tryptase) participate in the acute inflammation and remodeling process of viral myocarditis. PMID: 14578624
    20. mast cell chymase and CPA have key roles in both the generation and degradation of Ang II PMID: 15173164
    21. key role for mast cell chymase in the regulation of pro-MMP-2 and -9 activities (mast cell chymase AND pro-mmp-2 AND -9) PMID: 15615702
    22. Mast cell protease 4 reduces the antibody response and the severity of disease in autoimmune arthritis. PMID: 19010978
    23. abdominal aortic aneurysm lesions in Mcpt4(-/-) mice had fewer inflammatory cells and less apoptosis, angiogenesis, and elastin fragmentation than those of WT mice PMID: 19720934
    24. chymase (MCP-4) present in the upper airways protects against allergic airway responses, possibly by regulating smooth muscle cells. PMID: 19841188

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  • 蛋白家族：
    Peptidase S1 family, Granzyme subfamily
  • 组织特异性：
    Submucosal mast cells. In femoral muscle, detected in myocytes but not in mast cells.
  • 数据库链接：

    KEGG: mmu:17227

    STRING: 10090.ENSMUSP00000038103

    UniGene: Mm.439684