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  4. Recombinant Mouse LIM domain kinase 1 (Limk1)

Recombinant Mouse LIM domain kinase 1 (Limk1)

  • 中文名称：
    小鼠Limk1重组蛋白
  • 货号：
    CSB-YP012952MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Limk1重组蛋白
  • 货号：
    CSB-EP012952MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Limk1重组蛋白
  • 货号：
    CSB-EP012952MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Limk1重组蛋白
  • 货号：
    CSB-BP012952MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Limk1重组蛋白
  • 货号：
    CSB-MP012952MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Limk1; Limk; LIM domain kinase 1; LIMK-1; EC 2.7.11.1; KIZ-1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-647
  • 氨基酸序列
    MRLTLLCCTW REERMGEEGS ELPVCASCGQ RIYDGQYLQA LNADWHADCF RCCECSVSLS HQYYEKDGQL FCKKDYWARY GESCHGCSEH ITKGLVMVAG ELKYHPECFI CLACGNFIGD GDTYTLVEHS KLYCGQCYYQ TVVTPVIEQI LPDSPGSHLP HTVTLVSIPA SAHGKRGLSV SIDPPHGPPG CGTEHSHTVR VQGVDPGCMS PDVKNSIHVG DRILEINGTP IRNVPLDEID LLIQETSRLL QLTLEHDPHD SLGHGPVSDP SPLSSPVHTP SGQAASSARQ KPVLRSCSID TSPGTSSLAS PASQRKDLGR SESLRVVCRP HRIFRPSDLI HGEVLGKGCF GQAIKVTHRE TGEVMVMKEL IRFDEETQRT FLKEVKVMRC LEHPNVLKFI GVLYKDKRLN FITEYIKGGT LRGIIKNMDS QYPWSQRVSF AKDIASGMAY LHSMNIIHRD LNSHNCLVRE NRNVVVADFG LARLMIDEKN QSEDLRSLKK PDRKKRYTVV GNPYWMAPEM INGRSYDEKV DVFSFGIVLC EIIGRVNADP DYLPRTMDFG LNVRGFLDRY CPPNCPPSFF PITVRCCDLD PEKRPSFVKL EQWLETLRMH LSGHLPLGPQ LEQLERGFWE TYRRGESSLP AHPEVPD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation. Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly. Stimulates axonal outgrowth and may be involved in brain development.
  • 基因功能参考文献：
    1. results demonstrate an important role for LIMKs in central sensitization and the development of chronic pain PMID: 28669900
    2. The spontaneous autosomal recessive mouse 'peroneal muscular atrophy' mutant (PMA) is a faithful morphological model of human clubfoot. The pma mutation was mapped and a candidate gene encoding LIM-domain kinase 1 (Limk1) identified, which is upregulated in mutant lateral motor neurons. PMID: 29439133
    3. High LIMK1 expression is associated with aberrant synaptic development in mouse and Drosophila models of Fragile X syndrome. PMID: 28465421
    4. we define how ROCK/LIMK pathway regulates mast cell development and functions PMID: 26943578
    5. increased BMPR2 signal transduction is linked to fragile X syndrome (FXS) and that the BMPR2-LIMK1 pathway is a putative therapeutic target in patients with FXS and possibly other forms of autism PMID: 27273096
    6. LIMK1 negatively regulated neuronal migration by affecting the neuronal cytoskeleton and that its effects were partly mediated by cofilin phosphorylation PMID: 27844143
    7. Social isolation resulted in up-regulation of Limk-1 mRNA expression in the cerebral cortex. PMID: 26921097
    8. The data emphasize the importance of the PLCeta2 EF-hand domain and articulation of PLCeta2 with LIMK-1 in regulating neuritogenesis. PMID: 26671787
    9. Our study indicates that there is a connection between LIMK1 and AD in the mouse model of AD. PMID: 26681218
    10. Neuroligin 1 regulates spines and synaptic plasticity via LIMK1/cofilin-mediated actin reorganization. PMID: 26880202
    11. LIMK1 and Cofilin phosphorylation depends on PKA and is essential for mouse sperm acrosomal exocytosis PMID: 26169470
    12. These results provide strong evidence that LIMK1 deletion is sufficient to lead to a long-term memory deficit and that this deficit is attributable to CREB hypofunction. PMID: 25645926
    13. the conserved region in the LIMK1 3' UTR is involved in regulating LIMK1 expression at the post-transcriptional level PMID: 23700283
    14. LIMK1 plays a novel role in selectively mediating GPIb-IX-dependent TXA2 synthesis and thrombosis. PMID: 23620575
    15. data support the hypothesis that LIMK1 is required for normal osteoblast differentiation. In addition, its absence leads to increased cytoskeletal remodeling and bone resorption in osteoclasts PMID: 23017662
    16. LIMK1 has a dual role in regulating lamellipodium extension by decelerating actin retrograde flow and polymerization. PMID: 21868383
    17. LIMK1 expression is more dynamic than previously reported, in particular at sites of tissue-tissue interactions guiding multiple developmental processes PMID: 21167960
    18. By activating the cofilin regulator Lim domain kinase 1 (Limk1), bone morphogenetic proteins control the rate of commissural axonal growth. PMID: 21084599
    19. Results suggest that constitutive activation of the Rho-ROCK-LIM kinase pathway by extracellular production of lysophosphatidic acid by the action of autotaxin is required to maintain the large size of lysosomes in visceral endoderm cells. PMID: 19808661
    20. role in actin cytoskeletal regulation PMID: 12073613
    21. Using LIMK-1 knockout mice, we show a significant role for LIMK-1 in vivo in regulating cofilin and the actin cytoskeleton. LIMK-1 plays a critical role in dendritic spine morphogenesis and brain function. PMID: 12123613
    22. LIM kinase 1 (LIMK1), a critical regulator of actin dynamics, plays a regulatory role in tumor cell invasion. PMID: 12777619
    23. LIMK-1/2 double knockout mice were more severely impaired in both ADF/cofilin phosphorylation and excitatory synaptic function in the CA1 region of the hippocampus. PMID: 15458846
    24. Results define a pathway linking the bone morphogenetic protein receptor BMPRII to regulation of actin and provides insights into how extracellular signals modulate LIMK1 activity during dendritogenesis. PMID: 15538389
    25. Rnf6 controls cellular LIMK1 concentrations and indicate a new function for the ubiquitin/proteasome system in regulating local growth cone actin dynamics PMID: 16204183
    26. LIMK1 phosphorylation of p25 blocks p25 activity, thus promoting microtubule disassembly. PMID: 18028908
    27. LIMK1-mediated cofilin phosphorylation is required for accurate spindle orientation by stabilizing cortical actin networks during mitosis. PMID: 18079118
    28. LIMK1 may mediate TGF-beta-dependent signaling during ocular inflammation. PMID: 18978953
    29. PKA is crucial in cell morphology and migration through its ability to modulate directly the activity of LIM kinase. PMID: 19424295
    30. improvement of endothelial barrier function along with impaired neutrophil chemotaxis were the underlying mechanisms that reduced severity of ALI in limk1(-/-) mice PMID: 19679840
    31. LIMK1-mediated cofilin phosphorylation is critical for ionomycin-induced neurite outgrowth and that CaMKIV mediates ionomycin-induced LIMK1 activation PMID: 19696021

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Cytoplasm, cytoskeleton. Cell projection, lamellipodium.
  • 蛋白家族：
    Protein kinase superfamily, TKL Ser/Thr protein kinase family
  • 组织特异性：
    Highest expression in the nervous system, particularly in the spinal cord and the cranial nerve and dorsal root ganglia.
  • 数据库链接：

    KEGG: mmu:16885

    STRING: 10090.ENSMUSP00000015137

    UniGene: Mm.15409