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  4. Recombinant Mouse LIM domain-binding protein 1 (Ldb1)

Recombinant Mouse LIM domain-binding protein 1 (Ldb1)

  • 中文名称：
    小鼠Ldb1重组蛋白
  • 货号：
    CSB-YP012829MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Ldb1重组蛋白
  • 货号：
    CSB-EP012829MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Ldb1重组蛋白
  • 货号：
    CSB-EP012829MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Ldb1重组蛋白
  • 货号：
    CSB-BP012829MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Ldb1重组蛋白
  • 货号：
    CSB-MP012829MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Ldb1
  • Uniprot No.：
  • 别名：
    Ldb1; Nli; LIM domain-binding protein 1; LDB-1; Carboxyl-terminal LIM domain-binding protein 2; CLIM-2; LIM domain-binding factor CLIM2; mLdb1; Nuclear LIM interactor
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-411
  • 氨基酸序列
    SVGCACPGC SSKSFKLYSP KEPPNGNAFP PFHPGTMLDR DVGPTPMYPP TYLEPGIGRH TPYGNQTDYR IFELNKRLQN WTEECDNLWW DAFTTEFFED DAMLTITFCL EDGPKRYTIG RTLIPRYFRS IFEGGATELY YVLKHPKEAF HSNFVSLDCD QGSMVTQHGK PMFTQVCVEG RLYLEFMFDD MMRIKTWHFS IRQHRELIPR SILAMHAQDP QMLDQLSKNI TRCGLSNSTL NYLRLCVILE PMQELMSRHK TYSLSPRDCL KTCLFQKWQR MVAPPAEPAR QQPSKRRKRK MSGGSTMSSG GGNTNNSNSK KKSPASTFAL SSQVPDVMVV GEPTLMGGEF GDEDERLITR LENTQFDAAN GIDDEDSFNN SPALGANSPW NSKPPSSQES KSENPTSQAS Q
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. Plays a role in the development of interneurons and motor neurons in cooperation with LHX3 and ISL1. Acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. Acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state.
  • 基因功能参考文献：
    1. These findings show that control of Lhx2 function by Ldb1 and Rnf12 underpins the coordinated differentiation of neurons and Muller glia in postnatal retina. PMID: 29650591
    2. Both LDB1 and CTCF are required for enhancer-Car2 looping, and the domain of LDB1 contacted by CTCF is necessary to rescue Car2 transcription in LDB1-deficient cells. PMID: 28636938
    3. Animals lacking both Ldb1 and Ldb2 uncover the requirement for Ldb2 during corticospinal motor neuron differentiation PMID: 26830346
    4. Data show that mediator is recruited to the beta-globin locus by the LDB1 complex. PMID: 28520978
    5. Genome-wide analysis indicated that LMO2 is required at the hemangioblast stage to position the TAL1/LMO2/LDB1 complex to regulatory elements that are important for the establishment of the hematopoietic developmental program. PMID: 28973433
    6. LDB1 regulates energy homeostasis, in part through transcriptional modulation of critical regulators in brown adipose tissue function. PMID: 28009534
    7. an essential cell-autonomous role for Ldb1 in the development of dopaminergic neurons, is reported. PMID: 27171818
    8. An intricate regulatory network exists that is mediated by Ldb1 and Ldb2, and promotes retinal progenitor cells proliferation and multipotency; it also controls specification of mammalian retina cells. PMID: 27697904
    9. LDB1 maintains the terminally differentiated state of beta cells and is a component of active enhancers in both murine and human islets. PMID: 27941246
    10. CLIM interacts with estrogen receptor alpha at the H19 locus, potentially explaining the higher expression of H19 in female than male corneas. PMID: 27129775
    11. SSBP3 Interacts With Islet-1 and Ldb1 to Impact Pancreatic beta-Cell Target Genes PMID: 26495868
    12. results indicate that LDB1-dependent looping events can deliver repressive cargo to cognate promoters to mediate promoter pausing events in a pituitary cell type PMID: 25605944
    13. Data suggest that the function of LIM domain binding protein 1 (LDB1) in the neural crest-derived palatal mesenchyme is essential for normal morphogenesis of the secondary palate. PMID: 24433583
    14. the LDB1 dimerization domain (DD) is necessary and, when fused to LMO2, sufficient to completely restore LCR-promoter looping and transcription in LDB1-depleted cells PMID: 24874989
    15. Ldb1 plays an essential role as a transcription co-regulator of Lhx6 and Lhx8 in the control of mammalian telencephalon development. PMID: 24157949
    16. These results provide a foundation for defining the mechanism and scope of Ldb1 complex activity during erythropoiesis. PMID: 23610375
    17. Data indicte that the regulation of essential developmental factors by Ldb1 defines it as an upstream transcriptional regulator of hematopoietic/endothelial development. PMID: 23390196
    18. Solution structure of the LIM-homeodomain transcription factor complex Lhx3/Ldb1 and the effects of a pituitary mutation on key Lhx3 interactions. PMID: 22848397
    19. Studied the solution structure of Lmo2(LIM2) /Ldb1(LID) complex. Results show modular binding of tandem LIM domains in Lmo2 to tandem linear motifs in Ldb1 is accompanied by several disorder-to-order transitions/ conformational changes in both proteins. PMID: 22936624
    20. Taken together, these data show that Ldb1 forms a regulatory complex to maintain Car1 expression in erythroid cells. PMID: 22609543
    21. Findings establish Ldb1 as a critical effector of GATA1-mediated loop formation and indicate that chromatin looping causally underlies gene regulation. PMID: 22682246
    22. controls transcriptional program required for stem cells maintenance by restricting their differentiation PMID: 21186366
    23. knockout mice demonstrated a significantly enhanced growth of liver cancer (HCC) by means of tumor size and number, advocating for an essential role of Ldb1 in HCC development PMID: 20828852
    24. a role for Ldb1 in erythropoiesis in vivo and establish a critical function for Ldb1-nucleated complexes in regulating the erythroid/megakaryocyte transcriptional program. PMID: 21041453
    25. Ldb1 stabilizes its erythroid complex partners on beta-globin chromatin, even though it is not one of the DNA-binding components. In addition, Ldb1 is necessary for enrichment of key transcriptional components in the locus. PMID: 20570862
    26. Data suggest that Ldb1/2 function to maintain SLK in an inactive state before its activation. PMID: 19675209
    27. Ldb1 plays a key regulatory role in transcriptional events that govern the biological process of epithelial tissue homeostasis in mammalian small intestine PMID: 19918297
    28. Functional ablation of the mouse Ldb1 gene results in severe patterning defects during gastrulation.There is no heart anlage and head structures are truncated anterior to the hindbrain. In about 40% of the mutants, posterior axis duplication is observed. PMID: 12490556
    29. Data show that a nuclear complex containing TAL1 and LIM domain-binding protein Ldb1 transactivates the protein 4.2 (P4.2) gene. PMID: 14560005
    30. mouse Ldb1b lacks the ability to activate protein 4.2 promoter, which is stimulated by LMO2 and Ldb1. These findings suggest that splice isoforms of Ldb lacking LID are potential regulators of Ldb function.[lbd1] PMID: 16815859
    31. Study identifies a number of novel Ldb1 interacting proteins in murine erythroleukaemic cells, namely Eto-2, the cyclin-dependent kinase Cdk9, Lmo4; morphilino-mediated knockdowns in zebrafish show these factors to be essential for haematopoiesis. PMID: 17108004
    32. We conclude that LDB1, but not E2A is a promising candidate as a modifier gene in patients with nail-patella syndrome. PMID: 17316599
    33. Lhx1 and Lhx5, and their cofactor Ldb1, control Purkinje cell differentiation in the developing cerebellum. PMID: 17664423
    34. Clim2 interacts with Lhx2 in vivo, suggesting that Clim2 is an essential co-factor for the LIM homeodomain factor Lhx2 PMID: 17991461
    35. Generation of Ldb1-null mutant (Ldb1-/-) ES cells and examinaion og neuronal differentiation potentials in vitro using two different neuronal differentiation protocols. PMID: 18388304

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    LDB family
  • 组织特异性：
    Expression overlaps that of LIM domain-containing proteins. Expressed widely in the embryo with highest expression in several regions of the brain the central nervous system ganglia. Also expressed in fetal liver, lung, kidney, thymus and olfactory epithe
  • 数据库链接：

    KEGG: mmu:16825

    STRING: 10090.ENSMUSP00000118546

    UniGene: Mm.327442