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  4. Recombinant Mouse Izumo sperm-egg fusion protein 1 (Izumo1), partial

Recombinant Mouse Izumo sperm-egg fusion protein 1 (Izumo1), partial

  • 中文名称：
    小鼠Izumo1重组蛋白
  • 货号：
    CSB-YP863556MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Izumo1重组蛋白
  • 货号：
    CSB-EP863556MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Izumo1重组蛋白
  • 货号：
    CSB-EP863556MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Izumo1重组蛋白
  • 货号：
    CSB-BP863556MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Izumo1重组蛋白
  • 货号：
    CSB-MP863556MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Izumo1
  • Uniprot No.：
  • 别名：
    Izumo1; Izumo sperm-egg fusion protein 1; Oocyte binding/fusion factor; OBF; Sperm-specific protein izumo
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'.
  • 基因功能参考文献：
    1. IZUMO1 interacts with JUNO during sperm binding.Trp62 of JUNO interacts with hydrophobic residues on the alpha-helical core region of IZUMO1. PMID: 27416963
    2. These data support the hypothesis that dynamic movement of IZUMO1 is essential for gamete fusion during mouse fertilization. PMID: 28833824
    3. The crystal structure of Izumo1 reveals a membrane distal region composed of a four-helix bundle connected to a carboxy-terminal immunoglobulin (Ig)-like domain through a beta-hairpin stabilized by disulfide bonds. Remarkably, different regions of Izumo1 display significant structural similarities to two proteins expressed by the invasive sporozoite stage of Plasmodium parasites: SPECT1, and TRAP. PMID: 27374339
    4. Mice without the cytoplasmic tail of IZUMO1 showed normal fertility but decreased the amount of protein, indicating that whilst this region is important for the expression level of IZUMO1, it is dispensable for fertilisation in the mouse PMID: 27624483
    5. Five IZUMO BCE were identified: DLVLDCL177-183, YSFYRV196-201 (named BCE-2), YLT217-219, SMVGPED221-227, and DAGNY228-232. Active immunization with the BCE-2 vaccine sharply decreased the fertility rate in female mice in a safe and reversible manner. PMID: 26782177
    6. Robust IZUMO1-dependent adhesion of sperm with an oocyte accompanies the dimerization of IZUMO1. PMID: 26568141
    7. Binding of sperm protein Izumo1 and its egg receptor Juno drives Cd9 accumulation in the intercellular contact area prior to fusion during mammalian fertilization. PMID: 25209248
    8. identification of folate receptor 4 (Folr4) as the receptor for Izumo1 on the mouse egg. PMID: 24739963
    9. 'IZUMO1 putative functional fragment (IZUMO1PFF) maintains binding ability on the egg surface of Cd9(-/-) eggs. IZUMO1PFF is composed of an N-terminal unfolded structure and a C-terminal ellipsoidal helix dimer. PMID: 23824580
    10. Translocation of IZUMO1 during the fertilization process determined by time lapse imaging. PMID: 22946049
    11. four phosphopeptide changes came from Izumo1, the sperm-egg fusion protein, in the cytoplasmic segment of the protein. 2D-PAGE confirmed that Izumo1 is post-translationally modified during epididymal transit. PMID: 22954305
    12. co-localization of TMEM190 with IZUMO1 and complex formation with other sperm proteins. PMID: 21273369
    13. identified an IZUMO1-interacting protein in sperm, which was identified as testis specific ACE homologue ACE3. PMID: 20421979
    14. Results suggest that Izumo 1 might be involved in organizing or stabilizing a multiprotein complex essential for the function of the membrane fusion machinery. PMID: 19658160
    15. identification of a mouse sperm fusion-related antigen and show that the antigen is a novel immunoglobulin superfamily protein PMID: 15759005
    16. These data suggest that glycosylation is not essential for the function of IZUMO, but has a role in protecting it from fragmentation in cauda epididymis. PMID: 18952059
    17. pCXN2-mIzumo plasmid possesses appreciable anti-fertility potential. PMID: 19239425
    18. Data indicate that the Ig-like domain of Izumo plays an important role in the fertilization process, as verified by the dose-dependent reduction in fertilization rates in mouse IVF trials and mouse mating assay. PMID: 19288544

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, secretory vesicle, acrosome membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Izumo family
  • 组织特异性：
    Sperm-specific (at protein level). Detectable on sperm surface only after the acrosome reaction.
  • 数据库链接：

    KEGG: mmu:73456

    STRING: 10090.ENSMUSP00000033100

    UniGene: Mm.380445