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  4. Recombinant Mouse Interleukin-27 subunit beta (Ebi3)

Recombinant Mouse Interleukin-27 subunit beta (Ebi3)

  • 中文名称：
    小鼠Ebi3重组蛋白
  • 货号：
    CSB-YP007360MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Ebi3重组蛋白
  • 货号：
    CSB-EP007360MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Ebi3重组蛋白
  • 货号：
    CSB-EP007360MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Ebi3重组蛋白
  • 货号：
    CSB-BP007360MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Ebi3重组蛋白
  • 货号：
    CSB-MP007360MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Ebi3
  • Uniprot No.：
  • 别名：
    Ebi3; Il27bInterleukin-27 subunit beta; IL-27 subunit beta; IL-27B; Epstein-Barr virus-induced gene 3 protein homolog
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    19-228
  • 氨基酸序列
    YT ETALVALSQP RVQCHASRYP VAVDCSWTPL QAPNSTRSTS FIATYRLGVA TQQQSQPCLQ RSPQASRCTI PDVHLFSTVP YMLNVTAVHP GGASSSLLAF VAERIIKPDP PEGVRLRTAG QRLQVLWHPP ASWPFPDIFS LKYRLRYRRR GASHFRQVGP IEATTFTLRN SKPHAKYCIQ VSAQDLTDYG KPSDWSLPGQ VESAPHKP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Associates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines.
  • 基因功能参考文献：
    1. Data indicate an increase in the intracellular Epstein-Barr virus-induced 3 (EBI3) protein level in splenic NK cells during mouse cytomegalovirus (MCMV) infection. PMID: 28143936
    2. IL-35-transduced adipocyte-derived mesenchymal stem cells in vivo can enhance proliferation of CD4(+) CD25(+) Treg cells and suppress the function of effector T cells such as T helper (Th) 1, Th2 and Th17 cells and may reduce the development of allograft rejection. PMID: 28888053
    3. we describe here a new IL-12 member IL-39 (IL-23p19/Ebi3) secreted by activated B cells that mediate inflammatory responses in lupus-like mice. Thus, IL-39 might contribute to immunopathogenic mechanisms of SLE. PMID: 27019190
    4. EBI3 can mediate IL-6 trans-signaling in a process that involves gp130 PMID: 28280243
    5. our results suggest that IL-39 induces differentiation and/or expansion of neutrophils in lupus-prone mice. PMID: 27400195
    6. Data show that interleukin-35 (Epstein-Barr virus-induced gene 3 (EBI3) and the interleukin-12 Subunit p35 (p35) subunit) expressing plasmid downregulates the expression of pro-inflammatory cytokines and upregulates the expression of anti-inflammatory cytokines. PMID: 27460435
    7. Data suggest that interleukin-35 (Epstein-Barr virus-induced gene 3 (EBI3) and the interleukin-12 Subunit p35 (p35) subunit) contributes to the increased susceptibility to secondary pneumococcal pneumonia. PMID: 26844658
    8. our results demonstrate that EBI3 is an essential general regulator of inflammatory immune responses in experimental Chagas disease PMID: 26694585
    9. Report higher EBI3 expression in the lung tissue of BCG-infected mice. PMID: 25937126
    10. EBI3 Downregulation Contributes to Type I Collagen Overexpression in Scleroderma Skin. PMID: 26355156
    11. IL-35 subunits (p35 and EBI3) have the immunoregulatory role in systemic lupus erythematosus. PMID: 25845911
    12. Lack of EBI3, p35, and IL-12 receptor beta2 caused enhanced infiltration of lymphocytes into the testicular interstitium, with increased interferon-gamma expression in the testes and autoantibody production against mainly acrosomal regions of spermatids. PMID: 24760014
    13. Data (including data from knockout mice) suggest Ebi3 (Epstein-Barr virus induced protein 3) and IL12 (interleukin 12) down-regulate helper-inducer T-lymphocyte (Th17 cell) function in immune response to Listeria monocytogenes. PMID: 24068935
    14. results indicate that EBI3 may be important in EAU initiation by Th1 cell responses and may suppress experimental autoimmune uveitis by inhibition of both Th1 and Th17 cell responses in the late/maintenance phase. PMID: 24929202
    15. Anti-CD200R2, anti-IL-9, anti-IL-35, or anti-TGF-beta abolishes increased graft survival and Treg induction induced in cromolyn-treated CD200R1KO.CD200tg mice, and suggest combined role of mast cells and Tregs. PMID: 24142033
    16. the findings from the past decade identify IL-27 as a critical immunoregulatory cytokine, especially for T cells, whereas some controversy is fueled by results challenging the view of IL-27 as a classical silencer of inflammation. PMID: 23904441
    17. These data demonstrate a regulatory role for EBI3-associated cytokines in controlling host responses following CNS viral infection. PMID: 23102608
    18. EBI3 plays an inhibitory role in Th17, Th1, IL-2, and Treg responses. PMID: 22387555
    19. Results provide the first evidence that EBI3 may play an inhibitory role in allergic asthma development. PMID: 21255010
    20. The expression of EBI3 mRNA and p28 mRNA was up-regulated at onset of EAE. PMID: 19060371
    21. These data clearly demonstrated the immunosuppressive function of EBI-3. PMID: 20064562
    22. EBI3 plays a critical regulatory role in the induction of Th2-type immune responses and the development of Th2-mediated tissue inflammation in vivo. PMID: 12482940
    23. EBI3 gene transcription in dendritic cells is induced rapidly by Toll-like receptor signaling during innate immune responses preceding cytokine driven T helper cell development. PMID: 15728491
    24. novel Ebi3-IL-12alpha heterodimeric cytokine has been designated interleukin-35;data identify IL-35 as a novel inhibitory cytokine that may be specifically produced by T(reg) cells and is required for maximal suppressive activity PMID: 18033300
    25. These data demonstrate a key pathogenic role of IL-27 in T cell-mediated liver injury. PMID: 18096999
    26. Data provide direct evidence that EBI3 negatively regulates T-cell expression of IL-17, IL-22 and RORgamma t, as well as protective immunity against L. monocytogenes. PMID: 18412165
    27. Loss of EBI3 thus favors Th2-type autoimmune responses, suggesting that the Th1/Th2 balance may be a pivotal determinant of phenotypes of human autoimmune diseases. PMID: 18779924
    28. Targeted deletion of EBI-3 protects mice from lung metastasis upon intravenous injection of transformed tumor cell line B16-F10. PMID: 18941205

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Type I cytokine receptor family, Type 3 subfamily
  • 数据库链接：

    KEGG: mmu:50498

    STRING: 10090.ENSMUSP00000003274

    UniGene: Mm.256798