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  4. Recombinant Mouse Interferon regulatory factor 8 (Irf8)

Recombinant Mouse Interferon regulatory factor 8 (Irf8)

  • 中文名称：
    小鼠Irf8重组蛋白
  • 货号：
    CSB-EP011823MO
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Irf8; Icsbp; Icsbp1; Interferon regulatory factor 8; IRF-8; Interferon consensus sequence-binding protein; ICSBP
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    64.2kDa
  • 表达区域：
    1-424aa
  • 氨基酸序列
    MCDRNGGRRLRQWLIEQIDSSMYPGLIWENDEKTMFRIPWKHAGKQDYNQEVDASIFKAWAVFKGKFKEGDKAEPATWKTRLRCALNKSPDFEEVTDRSQLDISEPYKVYRIVPEEEQKCKLGVAPAGCMSEVPEMECGRSEIEELIKEPSVDEYMGMTKRSPSPPEACRSQILPDWWVQQPSAGLPLVTGYAAYDTHHSAFSQMVISFYYGGKLVGQATTTCLEGCRLSLSQPGLPKLYGPDGLEPVCFPTADTIPSERQRQVTRKLFGHLERGVLLHSNRKGVFVKRLCQGRVFCSGNAVVCKGRPNKLERDEVVQVFDTNQFIRELQQFYATQSRLPDSRVVLCFGEEFPDTVPLRSKLILVQVEQLYARQLVEEAGKSCGAGSLMPALEEPQPDQAFRMFPDICTSHQRPFFRENQQITV
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor that specifically binds to the upstream regulatory region of type I interferon (IFN) and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Can both act as a transcriptional activator or repressor. Plays a negative regulatory role in cells of the immune system. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes. Required for the development of plasmacytoid dendritic cells (pDCs), which produce most of the type I IFN in response to viral infection. Positively regulates macroautophagy in dendritic cells.
  • 基因功能参考文献：
    1. breast and pancreatic tumor-produced granulocyte-stimulating factor downregulates interferon regulatory factor-8 in conventional dendritic cells (cDC) progenitors, and thus results in reduced cDC1 development PMID: 29593283
    2. Study shows that IRF8 is required for Th9 differentiation in vitro and in vivo. IRF8 functions through a transcription factor complex consisting of IRF8, IRF4, PU.1 and BATF, which binds to DNA and boosts Il9 transcription. PMID: 29233972
    3. Expression of IRF-8 is significantly elevated in the microglia of AD mice. Silencing of IRF-8 abolished Abeta1-40-induced microglia activation. PMID: 28856571
    4. DNA methylation plays a protective role in cisplatin-induced acute kidney injury by regulating specific genes, such as Irf8. PMID: 28709638
    5. IRF8 does not play an essential intrinsic role in Th17 cell differentiation. PMID: 27140932
    6. frequencies of antigen-experienced CD4+CD11ahiCD49dhi cells that were CD44hiCD62L- were similar in MLN of infected Irf8-/- and B6 mice, but the proportions of CD4+GATA3+ and CD4+IL-4+ T cells were lower in infected Irf8-/- mice PMID: 28968468
    7. Mysm1 enhanced function of the IRF2 and IRF8 promoters, suggesting that Mysm1 governs the IRFs for hematopoietic stem cell homeostasis. PMID: 27277682
    8. results show that polymorphonuclear-myeloid-derived suppressor cells arise from a newly defined granulocyte progenitors (GP) stage within the bone marrow and that IRF8 levels (and/or their downstream target genes) in those GPs guide their expansion or contraction PMID: 28356386
    9. IRF8 differentially controls the survival. PMID: 27637148
    10. IRF8 might play a role in restraining excess lymphocyte proliferation. PMID: 27171004
    11. IRF8 controls Th1 immune response in Treg cells independent of T-bet. PMID: 26166768
    12. The s further identified a distinct molecular signature of F4/80(hi) and CD11b(hi) macrophages and found that Irf8 was vital for macrophage maturation. PMID: 27412700
    13. this study shows that myeloid-specific SIRT1 restrains pro-inflammatory processes by deacetylating IRF8 PMID: 28008797
    14. IRF8 was a target of miR-451a in vitro and in vivo. The data indicate the function and a target of miR-451a in SLE PMID: 28120198
    15. the mechanism for the observed differential induction of the mouse Ifit1, Ifit2, and Ifit3 genes in B cells and demonstrated that the repressive effect of the transcription factor interferon regulatory factor 8 (IRF8), which is highly expressed in B cells, played an essential role in this regulation. PMID: 27137933
    16. direct the expression of a set of genes, the IRF8/IRF1 regulome, that play critical roles in host inflammatory and antimicrobial defenses in mouse models of neuroinflammation and of pulmonary tuberculosis, respectively PMID: 27001747
    17. we identified IRF8 target genes in gastric epithelial cells, providing a detailed understanding of how IRF8 functions in gastric mucosal innate immunity PMID: 26843324
    18. results suggest that impaired Icsbp expression enhances leukemogenesis by deregulating processes that normally limit granulocyte expansion during the innate immune response. PMID: 26683374
    19. variation in IRF4 or IRF8 levels resulting from genetic polymorphisms or environmental cues will govern tissue dendritic cells numbers and regulate the magnitude of their functional responses. PMID: 26746189
    20. It plays a crucial role in the transformation of microglia to a reactive state by regulating the expression of various genes. PMID: 26318672
    21. Data indicate that interferon regulatory factor-8 (IRF8) regulates tumor behavior in an matrix metalloproteinase 3 (MMP3)-dependent manner. PMID: 26008967
    22. Data indicate that interferon regulatory factors IRF4 and IRF8 control distinct activated B cell states. PMID: 26437243
    23. dendritic cells from mice with CD11c-specific constitutive b-catenin activation upregulated IRF8 through targeting of the Irf8 promoter, leading to in vivo expansion of IRF8-dependent CD8alpha+, plasmacytoid, and CD103+ CD11b2 dendritic cells. PMID: 25416805
    24. Loss of IRF8 in T Cells Exacerbates Uveitis, Whereas Irf8 Deletion in the Retina Confers Protection PMID: 26163590
    25. Data show that IRF8 directly interacts with NFATc1 to prevent NFATc1 translocation and thus inhibits the hypertrophic response. PMID: 24526256
    26. we concluded that the IRF-8 KO cells performed pure uncorrelated random walks, while WT splenocytes were able to make singular drifted random walks that collapsed on a straight ballistic motion for the system as a whole PMID: 25322144
    27. IRF5 expression in microglia is regulated by IRF8. IRF5 directly upregulates P2rX4 expression on microglia in peripheral nerve injury, and may play a role in neuropathic pain. PMID: 24818655
    28. Data indicte that interferon regulatory factor 8 (IRF8) expression becomes dependent on transcription factor Batf3. PMID: 26054719
    29. These data highlight a critical role for IRF8 in inflammatory monocyte differentiation and migration during WNV infection. PMID: 25277331
    30. IRF8 is a major autophagy regulator in macrophages, essential for macrophage maturation, survival and innate immune responses PMID: 25775030
    31. plays a key role in the cross-talk between melanoma and immune cells delimiting tumor spread PMID: 24597645
    32. Irf8-knockout microglia exhibited a differential expression pattern of nucleotide-degrading enzymes compared to their wild-type counterparts. PMID: 24798612
    33. Basal IRF8-PU.1 binding maintained the expression of a broad panel of genes essential for macrophage functions (such as microbial recognition and response to purines) and contributed to basal expression of many LPS-inducible genes PMID: 25637355
    34. we demonstrate that interferon regulatory factor-8 regulates basophil and mast cell development PMID: 25398936
    35. The transcription factors IRF8 and PU.1 negatively regulate plasma cell differentiation. PMID: 25288399
    36. The overexpressed IRF8 can effectively inhibit the differentiation of RAW264.7 cells into osteoclast-like cells. PMID: 24606735
    37. we found that the transcriptional factor IRF8 plays a protective role in the cerebral ischaemic-reperfusion injury by attenuating neuronal apoptosis PMID: 24528256
    38. IRF8 enhanced alphavbeta8 integrin expression in APCs and activated TGF-beta signaling leading to T helper 17 (Th17) cell differentiation PMID: 24485804
    39. Data indicate that interferon regulatory factor 8 (IRF8) inhibited smooth muscle cells (SMCs) marker gene expression through regulating serum response factor (SRF) transactivation in a myocardin-dependent manner. PMID: 24248596
    40. Data from mutant/knockout mice suggest that Irf8 is involved in innate immunity against Plasmodium berghei cerebral malaria; neuronal gene expression up-regulated by Plasmodium berghei infection favors proteins with IRF8 binding sites. PMID: 23853600
    41. IRF-8 is an essential factor for the maintenance of proper immune cell recruitment in granulomas. PMID: 23717393
    42. IRF8-deficient mice produced anti-dsDNA Abs. In a double-transgenic model, B cell anergy was breached in IRF8-deficient mice. Anergic B cells in the IRF8-deficient background were able to mature further & regain responses to Ag stimulation. PMID: 24218455
    43. Irf8 expression in DC9 cells led to an increase in Id2 & Batf3 transcript levels. Without it, Id2 & Batf3 expression was insufficient for directing classical CD8alpha(+) DC development. PMID: 24227775
    44. IRF8-rescued BCR-ABL-expressing dendritic cells were capable of inducing CTLs more efficiently than control dendritic cells. IRF8 is an attractive target in next-generation therapies for chronic myelogenous leukemia. PMID: 24242069
    45. These results suggest a central role for IFN regulatory factor 8-expressing CD8(+) DCs in governing the TLR11- and TLR12-mediated host defense against Toxoplasma gondii. PMID: 24078692
    46. Irf8 Expression Is Required for Transcriptional Identity of Early DC Progenitors. PMID: 23623495
    47. Irf8-deficient mice generate myeloid populations highly homologous to tumor-induced myeloid-derived suppressor cells. PMID: 24091328
    48. MiR-22 binds directly to the 3'UTR of the mouse Irf8 mRNA. PMID: 23251709
    49. Data indicate that Bcr-abl increases PTPN13 promoter activity in an Icsbp-dependent manner. PMID: 22891763
    50. these results underscore a key role of IRF-8 in the cross talk between melanoma and immune cells, thus revealing its critical function within the tumor microenvironment in regulating melanoma progression and invasiveness. PMID: 23308054

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    IRF family
  • 组织特异性：
    Mainly expressed in lymphoid tissues. Predominantly expressed in CD8(+)-expressing dendritic cells.
  • 数据库链接：

    KEGG: mmu:15900

    STRING: 10090.ENSMUSP00000040245

    UniGene: Mm.334861