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  4. Recombinant Mouse Inhibitor of nuclear factor kappa-B kinase subunit epsilon (Ikbke)

Recombinant Mouse Inhibitor of nuclear factor kappa-B kinase subunit epsilon (Ikbke)

  • 中文名称：
    小鼠Ikbke重组蛋白
  • 货号：
    CSB-YP886425MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Ikbke重组蛋白
  • 货号：
    CSB-EP886425MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Ikbke重组蛋白
  • 货号：
    CSB-EP886425MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Ikbke重组蛋白
  • 货号：
    CSB-BP886425MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Ikbke重组蛋白
  • 货号：
    CSB-MP886425MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ikbke; Ikke; IkkiInhibitor of nuclear factor kappa-B kinase subunit epsilon; I-kappa-B kinase epsilon; IKK-E; IKK-epsilon; IkBKE; EC 2.7.11.10; Inducible I kappa-B kinase; IKK-i
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-717
  • 氨基酸序列
    MQSTTNYLWH TDDLLGQGAT ASVYKARNKK SGEVVAVKVF NSASYRRPPE VQVREFEVLR RLNHQNIVKL FAVEETGGSR QKVLIMEYCS SGSLLSVLED PENTFGLSEE EFLVVLRCVV AGMNHLRENG IVHRDIKPGN IMRLVGEEGQ SIYKLSDFGA ARKLDDDEKF VSVYGTEEYL HPDMYERAVL RKPQQKAFGV TVDLWSIGVT LYHAATGSLP FIPFGGPRRN KEIMYRITTE KPAGAISGTQ KQENGPLEWS YSLPITCRLS MGLQNQLVPI LANILEVEED KCWGFDQFFA ETSDILQRTV IHVFSLPQAV LHHVYIHAHN TIAIFLEAVY EQTNVTPKHQ EYLFEGHPCV LEPSLSAQHI AHTAASSPLT LFSMSSDTPK GLAFRDPALD VPKFVPKVDL QADYSTAKGV LGAGYQALWL ARVLLDGQAL MLRGLHWVLE VLQDTCQQTL EVTRTALLYL SSSLGTERFS SGAGMPDVQE RKEATELRTR LQTLSEILSK CSHNVTETQR SLSCLGEELL KNRDQIHEDN KSIQKIQCCL DKMHFIYKQF KKSRMRPGLS YNEEQIHKLD KVNFSHLAKR LLQVFQEECV QTYQVSLVTH GKRMRQVQRA QNHLHLIGHS VATCNSEARG AQESLNKIFD QLLLDRASEQ GAEVSPQPMA PHPGPDPKDL VFHMQELCND MKLLAFDLQD NNRLIERLHR VPSAPDV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine/threonine kinase that plays an essential role in regulating inflammatory responses to viral infection, through the activation of the type I IFN, NF-kappa-B and STAT signaling. Also involved in TNFA and inflammatory cytokines, like Interleukin-1, signaling. Following activation of viral RNA sensors, such as RIG-I-like receptors, associates with DDX3X and phosphorylates interferon regulatory factors (IRFs), IRF3 and IRF7, as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRF3 leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNB. In order to establish such an antiviral state, IKBKE forms several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including IPS1/MAVS, TANK, AZI2/NAP1 or TBKBP1/SINTBAD can be recruited to the IKBKE-containing-complexes. Activated by polyubiquitination in response to TNFA and interleukin-1, regulates the NF-kappa-B signaling pathway through, at least, the phosphorylation of CYLD. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. In addition, is also required for the induction of a subset of ISGs which displays antiviral activity, may be through the phosphorylation of STAT1 at 'Ser-708'. Phosphorylation of STAT1 at 'Ser-708' seems also to promote the assembly and DNA binding of ISGF3 (STAT1:STAT2:IRF9) complexes compared to GAF (STAT1:STAT1) complexes, in this way regulating the balance between type I and type II IFN responses. Protects cells against DNA damage-induced cell death. Also plays an important role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity. Phosphorylates AKT1.
  • 基因功能参考文献：
    1. IKKepsilon promotes NFATc1 phosphorylation and inhibits T cell responses, identifying IKKepsilon as a crucial negative regulator of T cell activation and a potential target for immunotherapy. PMID: 27346349
    2. Virus-activated kinase IKKepsilon phosphorylated YAP at Ser403 and thereby triggered degradation of YAP in lysosomes and, consequently, relief of YAP-mediated inhibition of the cellular antiviral response. PMID: 28481329
    3. REGgamma-mediated control of IkappaBvarepsilon contributes to NFkappaB activation and promotes bowel inflammation and associated tumour formation in response to chronic injury PMID: 26899380
    4. The results highlight the importance of the C-terminal region in the functional activity of IKKepsilon in innate immune response PMID: 24722368
    5. tic ablation of IKBKE resulted in enhanced and prolonged priming of the NLRP3 inflammasome in cultured macrophages, in hypertrophic adipose tissue, and in livers of hypercholesterolemic mice. PMID: 25540417
    6. novel insight into IKKepsilon, which is involved in adipose tissue inflammation during the development of obesity PMID: 25130737
    7. miR-339-5p inhibits expression of the pro-inflammatory factors in alcohol-induced brain inflammation through the NF-kappaB pathway by abolishing IKK-beta and IKK-epsilon activity. PMID: 25172655
    8. IKKepsilon deficiency inhibits inflammation and induces cardiac protection in high-fat diet-induced obesity in mice. PMID: 24789209
    9. IKKepsilon is expressed in neurons and is upregulated in the hypothalamus of obese mice, contributing to insulin and leptin resistance. PMID: 24812431
    10. Data indicate the role for immune-response functions of IkappaBepsilon in B cells; it regulates proliferative capacity via at least two mechanisms involving cRel- and RelA-containing NF-kappaB dimers. PMID: 24591377
    11. IKKepsilon knockout prevents high fat diet induced arterial atherosclerosis and NF-kappaB signaling in mice. PMID: 23741427
    12. IKBKE regulates FOXO3a primarily through phosphorylation of SerS644 PMID: 23691078
    13. IKKvarepsilon might play an important role in ox-LDL-induced injury of endothelial cells. PMID: 23261326
    14. IKKi deficiency exacerbates cardiac hypertrophy by regulating the AKT and NF-kappaB signaling pathway PMID: 23349709
    15. Upon IL-1 stimulation, IKKi was activated to mediate GSK3alpha phosphorylation at S21. PMID: 23142783
    16. Endotoxin uses a distinct pathway to initiate low-grade inflammation in macrophages, requiring interaction with IRAK-1 upstream of inducible kappa B kinase epsilon (inducible kappa B kinase epsilon) to contribute to the induction of C/EBPdelta and proinflammatory mediators. PMID: 21357541
    17. IkappaB kinase epsilon (IKK(epsilon)) regulates the balance between type I and type II interferon responses. PMID: 22171011
    18. we concluded that IKKepsilon modulates inflammatory nociceptive sensitivity PMID: 21813779
    19. these data indicate that IKKepsilon does not have general pro-inflammatory properties in liver and adipose tissue, and suggest that reduced adiposity is the primary mechanism for improved insulin sensitivity in IKKepsilon(-/-) mice on chow diet. PMID: 21371440
    20. these data suggest alternative splicing as a novel regulatory mechanism suitable to shift the balance between different functions of IKKepsilon. PMID: 21182093
    21. IKKi/IKKepsilon plays a key role in integrating signals induced by pro-inflammatory stimuli. PMID: 12736252
    22. In stimulated murine fibroblast-like synoviocyte cultures, induction of matrix metalloproteinases MMP3 and MMP13 is significantly greater in the IKK epsilon-positive cells compared with IKK epsilon-deficient cultures. PMID: 15879144
    23. Ikbke is essential and for the regulation of IFN responses in innate immune cells except plasmacytoid DCs. PMID: 17056502
    24. Antiviral gene expression was also reduced in FLS from IKKepsilon(-/-) mice compared with IKKepsilon(+/+) mice. PMID: 17328045
    25. findings demonstrate that IKKepsilon is activated by IFNbeta & that IKKepsilon directly phosphorylates STAT1, a component of ISGF3; we conclude that IKKepsilon plays a critical role in IFN-inducible antiviral transcriptional response PMID: 17332413
    26. Gene expression analyses indicate that IKKepsilon knockout reduces expression of inflammatory cytokines, and changes expression of certain regulatory proteins and enzymes involved in glucose and lipid metabolism PMID: 19737522

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Nucleus, PML body.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family, I-kappa-B kinase subfamily
  • 组织特异性：
    Expressed in bone marrow-derived macrophages and at low levels in liver and white adipose tissue (at protein level). Detected in muscle and lung.
  • 数据库链接：

    KEGG: mmu:56489

    STRING: 10090.ENSMUSP00000054126

    UniGene: Mm.386783