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  4. Recombinant Mouse Inactive serine/threonine-protein kinase VRK3 (Vrk3)

Recombinant Mouse Inactive serine/threonine-protein kinase VRK3 (Vrk3)

  • 货号：
    CSB-YP809103MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP809103MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP809103MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP809103MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP809103MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Vrk3
  • Uniprot No.：
  • 别名：
    Vrk3Inactive serine/threonine-protein kinase VRK3; Serine/threonine-protein pseudokinase VRK3; Vaccinia-related kinase 3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-453
  • 氨基酸序列
    MISFCPVCGK SVKVSFKFCP YCGKALPVEE DGGTQSAVTP HVSSVPGSRR DLNSSFETSP KKVKCSHTVT SLPLSRHSDC DSSGSDNTLT SPDRATGTRS RPLTPKGSPL SNRQSPQTLK RTRVTTSLQA LATGTELTDQ NGKHWTLGAL QIRDDQGILY EAEPTSAVPS ESRTQKWRFS LKLDSKDGRL FNEQNFFQRV AKPLQVNKWK KQFLLPLLAI PTCIGFGIHQ DKYRFLVFPS LGRSLQSALD DNPKHVVSER CVLQVACRLL DALEYLHENE YVHGNLTAEN VFVNPEDLSQ VTLVGYGFTY RYCPGGKHVA YKEGSRSPHD GDLEFISMDL HKGCGPSRRS DLQTLGYCML KWLYGSLPWT NCLPNTEKIT RQKQKYLDSP ERLVGLCGRW NKASETLREY LKVVMALNYE EKPPYATLRN SLEALLQDMR VSPYDPLDLQ MVP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Inactive kinase that suppresses ERK activity by promoting phosphatase activity of DUSP3 which specifically dephosphorylates and inactivates ERK in the nucleus.
  • 基因功能参考文献：
    1. Stress-induced nuclear translocation of CDK5 suppresses neuronal death by downregulating ERK activation via VRK3 phosphorylation PMID: 27346674
    2. Finally, downregulation of hvrk3 using siRNA reduced the amplitude and lengthened the period of the cellular bioluminescence rhythm. Taken together, these data suggest that VRK3 plays a role in setting the amplitude and period length of circadian rhythms in mammalian cells. PMID: 28412365
    3. These data suggest that VRK3-mediated phosphorylation of BAF may facilitate DNA replication or gene expression by facilitating the dissociation of nuclear envelope proteins and chromatin during interphase. PMID: 25899223
    4. various other rodent adult tissues and embryos possess a common signaling mechanism which is involved in an indirect regulation of ERK activity by VRK3-mediated vaccinia H1-related (VHR) phosphatase activity PMID: 18035061
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Protein kinase superfamily, CK1 Ser/Thr protein kinase family, VRK subfamily
  • 组织特异性：
    Expressed in liver, kidney, muscle, thymus, and bone marrow. Weakly expressed in spleen.
  • 数据库链接：

    KEGG: mmu:101568

    STRING: 10090.ENSMUSP00000002275

    UniGene: Mm.28816