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  4. Recombinant Mouse Importin subunit alpha-2 (Kpna2)

Recombinant Mouse Importin subunit alpha-2 (Kpna2)

  • 中文名称：
    小鼠Kpna2重组蛋白
  • 货号：
    CSB-YP012484MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Kpna2重组蛋白
  • 货号：
    CSB-EP012484MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Kpna2重组蛋白
  • 货号：
    CSB-EP012484MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Kpna2重组蛋白
  • 货号：
    CSB-BP012484MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Kpna2重组蛋白
  • 货号：
    CSB-MP012484MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Kpna2
  • Uniprot No.：
  • 别名：
    Kpna2; Rch1; Importin subunit alpha-1; Importin alpha P1; Karyopherin subunit alpha-2; Pendulin; Pore targeting complex 58 kDa subunit; PTAC58; RAG cohort protein 1; SRP1-alpha
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-529
  • 氨基酸序列
    STNENANLP AARLNRFKNK GKDSTEMRRR RIEVNVELRK AKKDEQMLKR RNVSSFPDDA TSPLQENRNN QGTVNWSVED IVKGINSNNL ESQLQATQAA RKLLSREKQP PIDNIIRAGL IPKFVSFLGK TDCSPIQFES AWALTNIASG TSEQTKAVVD GGAIPAFISL LASPHAHISE QAVWALGNIA GDGSAFRDLV IKHGAIDPLL ALLAVPDLST LACGYLRNLT WTLSNLCRNK NPAPPLDAVE QILPTLVRLL HHNDPEVLAD SCWAISYLTD GPNERIEMVV KKGVVPQLVK LLGATELPIV TPALRAIGNI VTGTDEQTQK VIDAGALAVF PSLLTNPKTN IQKEATWTMS NITAGRQDQI QQVVNHGLVP FLVGVLSKAD FKTQKEAAWA ITNYTSGGTV EQIVYLVHCG IIEPLMNLLS AKDTKIIQVI LDAISNIFQA AEKLGETEKL SIMIEECGGL DKIEALQRHE NESVYKASLN LIEKYFSVEE EEDQNVVPET TSEGFAFQVQ DGAPGTFNF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
  • 基因功能参考文献：
    1. Results demonstrated that radiation-induced dying colorectal cancer cells released considerable amounts of KPNA2 that induce the maturation and activation of DCs for synergistic antitumor effect of radiation. PMID: 27107455
    2. mTORC1 positively regulated the importer protein KPNA2, which participated in glycolysis regulation downstream of mTORC1 in a HIF1alpha-independent manner, indicating that mTORC1 regulates glycolysis through multiple ways. PMID: 26844761
    3. provided support for a link between autophagy and epithelial-to-mesenchymal (-like) transition status in WT TP53 glioblastoma cells and provided evidence for the signaling pathway (MIR517C-KPNA2-cytoplasmic TP53) involved in attenuating autophagy PMID: 26553592
    4. Structure of importin-alpha bound to a non-classical nuclear localization signal of the influenza A virus nucleoprotein has been reported. PMID: 26456934
    5. Specific interaction with the nuclear transporter importin alpha2 can modulate paraspeckle protein 1 delivery to nuclear paraspeckles. PMID: 25694451
    6. constitutive expression of Kpna2 during the differentiation culture of ESCs significantly impairs clock development, and KPNA2 facilitates cytoplasmic localization of PER1/ PMID: 25389311
    7. a significant correlation of KPNA2 expression and tumour aggressiveness in a large variety of other solid tumour entities PMID: 24930886
    8. study reports a cell-fate determination mechanism in which importin alpha2 negatively regulates the nuclear import of certain transcription factors to maintain embryonic stem cell properties PMID: 23906064
    9. importin alpha binds to Nup153 PMID: 22510057
    10. results collectively reveal that nuclear-localized importin alpha2 influences gene expression and contributes directly to cell fate outcomes including non-apoptotic cell death. PMID: 21964068
    11. Chrp, Arip3, and Hop2 are binding partners of IMPalpha2 in the developmental context of germ line development. PMID: 21900684
    12. Overexpression of a dominant-negative IMPalpha2 isoform, when assessed against adjacent untransfected or IMPalpha2 transfected cells, led to both a significant reduction in endogenous Oct3/4 protein levels and inhibition of Oct3/4 nuclear localization. PMID: 21840941
    13. knockdown of KPNA2 decreased myotube growth. PMID: 21741962
    14. The results suggest that Klf2 and Klf4 function redundantly to drive high level of Kpna2 expression in ES cells. PMID: 21664354
    15. Study identified the sequences KKKRR, KKKRK, and KKRKK as the optimal sequences for binding to this site for mouse importin-alpha2, human importin-alpha1, and human importin-alpha5, respectively. PMID: 20406804
    16. Karyopherin alpha2: a control step of glucose-sensitive gene expression in hepatic cells PMID: 11988093

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Importin alpha family
  • 组织特异性：
    Slightly detected in brain but not in cerebellum and skeletal muscle, highly expressed in testis and spleen.
  • 数据库链接：

    KEGG: mmu:16647

    STRING: 10090.ENSMUSP00000137310

    UniGene: Mm.12508