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  4. Recombinant Mouse Immunoglobulin J chain (Jchain)

Recombinant Mouse Immunoglobulin J chain (Jchain)

In Stock
  • 中文名称：
    小鼠Jchain重组蛋白
  • 货号：
    CSB-EP011337MO
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    Jchain
  • Uniprot No.：
  • 别名：
    Jchain; IgjImmunoglobulin J chain
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    31.7kDa
  • 表达区域：
    22-159aa
  • 氨基酸序列
    GDDEATILADNKCMCTRVTSRIIPSTEDPNEDIVERNIRIVVPLNNRENISDPTSPLRRNFVYHLSDVCKKCDPVEVELEDQVVTATQSNICNEDDGVPETCYMYDRNKCYTTMVPLRYHGETKMVQAALTPDSCYPD
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    CUSABIO synthesized the recombinant gene by integrating the N-terminal 6xHis-SUMO tag sequence into the targeted gene encoding the 22-159aa of the mouse Jchain. The synthesized gene was subsequently cloned into an expression vector. After cloning, the expression vector was introduced into the E.coli for expression. The product was purified to obtain the recombinant mouse Jchain protein carrying N-terminal 6xHis-SUMO tag. The SDS-PAGE assayed the purity of this recombinant Jchain protein greater than 90%. This Jchain protein migrated along the gel to a band of about 30 kDa molecular weight.

    Immunoglobulin J chain is a protein encoding by a gene named Jchain in mouse and a gene named JCHAIN in human. This protein is a protein component of the antibodies IgM and IgA. The J chain-joined dimer is a nucleating unit for the IgM pentamer. And it induces dimers and/or larger polymers in the case of IgA. Moreover, it also helps to bind these immunoglobulins to secretory component. The diseases involved JCHAIN include Alpha Chain Disease and Climatic Droplet Keratopathy. Additionally, a study have reported that the upregulation of JCHAIN was likely related to tumor aggression, as studied in a cohort of patients with acute lymphoblastic leukemia (ALL).

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces dimers and/or larger polymers. It also helps to bind these immunoglobulins to secretory component.
  • 基因功能参考文献：
    1. Data show that Crlz1 increased the IgJ enhancer activity due to its CBF sites, as verified by both the absence of Crlz1 effect on the CBF-site mutated IgJ enhancer and the presence of transcriptional synergy between Crlz1 and CBFbeta. PMID: 19735751
    2. identifies the HSS3/4 enhancer of Crlz1-IgJ locus as another target of EBG in early developmental stage of B cells PMID: 16962668
    3. H3 and H4 histones toward the chromatin of the Crlz1 gene are found to be hyperacetylated, especially on H3, in the pre-B cells, whereas those toward the chromatin of the IgJ gene are found to be hyperacetylated in the plasma cells PMID: 17015728
  • 亚细胞定位：
    Secreted.
  • 数据库链接：

    KEGG: mmu:16069

    STRING: 10090.ENSMUSP00000084259

    UniGene: Mm.1192