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  4. Recombinant Mouse Homeobox protein CDX-2 (Cdx2)

Recombinant Mouse Homeobox protein CDX-2 (Cdx2)

  • 中文名称：
    Recombinant Mouse Homeobox protein CDX-2(Cdx2),Yeast
  • 货号：
    CSB-YP005128MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse Homeobox protein CDX-2(Cdx2),Yeast
  • 货号：
    CSB-EP005128MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse Homeobox protein CDX-2(Cdx2),Yeast
  • 货号：
    CSB-EP005128MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse Homeobox protein CDX-2(Cdx2),Yeast
  • 货号：
    CSB-BP005128MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse Homeobox protein CDX-2(Cdx2),Yeast
  • 货号：
    CSB-MP005128MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Cdx2
  • Uniprot No.：
  • 别名：
    Cdx2; Cdx-2Homeobox protein CDX-2; Caudal-type homeobox protein 2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-311
  • 氨基酸序列
    MYVSYLLDKD VSMYPSSVRH SGGLNLAPQN FVSPPQYPDY GGYHVAAAAA ATANLDSAQS PGPSWPTAYG APLREDWNGY APGGAAAANA VAHGLNGGSP AAAMGYSSPA EYHAHHHPHH HPHHPAASPS CASGLLQTLN LGPPGPAATA AAEQLSPSGQ RRNLCEWMRK PAQQSLGSQV KTRTKDKYRV VYTDHQRLEL EKEFHFSRYI TIRRKSELAA TLGLSERQVK IWFQNRRAKE RKIKKKQQQQ QQQQQQQPPQ PPPQPSQPQP GALRSVPEPL SPVTSLQGSV PGSVPGVLGP AGGVLNSTVT Q
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor which regulates the transcription of multiple genes expressed in the intestinal epithelium. Binds to the promoter of the intestinal sucrase-isomaltase SI and activates SI transcription. Binds to the DNA sequence 5'-ATAAAAACTTAT-3' in the promoter region of VDR and activates VDR transcription. Binds to and activates transcription of LPH. Activates transcription of CLDN2 and intestinal mucin MUC2. Binds to the 5'-AATTTTTTACAACACCT-3' DNA sequence in the promoter region of CA1 and activates CA1 transcription. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. Binds preferentially to methylated DNA.
  • 基因功能参考文献：
    1. Results reveal an early regulatory switch in Cdx2 expression and show that different inputs are needed to drive Cdx2 expression in the blastocyst trophectoderm and in trophoblast stem cells. PMID: 27256674
    2. AMPK enhances intestinal barrier function and epithelial differentiation via promoting CDX2 expression, which is partially mediated by altered histone modifications in the Cdx2 promoter. PMID: 28234358
    3. CDX2(Null)/BRAF(V600E) expression in adult mouse intestinal epithelium led to serrated morphology tumors (including carcinomas) and BRAF(V600E) potently interacted with CDX2 silencing to alter gene expression. Like human serrated lesions, CDX2(Null)/BRAF(V600E)-mutant epithelium expressed gastric markers. PMID: 28072391
    4. including Wnt and Fgf pathway components active in the axial progenitor niche. Our data demonstrate that integration of the Cdx/Hox and T Brachyury transcriptional networks controls differential axial growth during vertebrate trunk elongation PMID: 28009287
    5. A new organ culture system for adult murine oesophagus is described. Using this, Cdx2 and HNF4alpha were ectopically expressed by adenoviral infection. We demonstrate the expression of CDX2 and HNF4alpha in human biopsy samples. PMID: 27875772
    6. Cdx members interact with the SWI-SNF complex and make direct contact with Brg1, a catalytic member of SWI-SNF. Both Cdx2 and Brg1 co-occupy a number of Cdx target genes, and both factors are necessary for transcriptional regulation of such targets. Finally, Cdx2 and Brg1 occupancy occurs coincident with chromatin remodeling at some of these loci. PMID: 28082674
    7. The s identify a Cdx2-responsive segment of HoxA, immediately 5' to the recently defined regulatory domain orchestrating initial transcription of the first Hox gene. PMID: 28041967
    8. this study shows that Aloe vera gel suppresses colitis-related colon carcinogenesis via increased expression of CDX-2, a tumor suppressor in colorectal cancer PMID: 27697726
    9. Results point out the complex interplay between the DSB DNA repair activity and the homeoproteins HoxB7 and Cdx2 in colon cancer cells. PMID: 26902420
    10. CDX2 is required for proliferation and differentiation of intestinal stem cells. Direct CDX2 targets in ISCs may be activated or repressed. PMID: 26489894
    11. cells in preimplantation embryos that localized in an initial outer position initiated the expression of Cdx2. cells that changed their position from an outer to an inner position downregulated Cdx2 expression and contributed to the inner cell mass. PMID: 26806703
    12. findings illuminated a role for NOD1 signaling in attenuating H. pylori-induced Cdx2 expression in gastric epithelial cells PMID: 26759244
    13. Overexpression of CDX2 inhibits the growth of the gastric cancer cells in vivo and in vitro. PMID: 25738600
    14. Intestinal stem cells transform into gastric stem cells on loss of transcription factor Cdx2. PMID: 25500896
    15. GATA4 and HNF4A control distinct aspects of intestinal homeostasis in conjunction with transcription factor CDX2 PMID: 25488664
    16. pSMAD/CDX2 interaction is essential for the switch toward an intestinal phenotype in Barrett's esophagus. PMID: 24794431
    17. Cdx2 is important for the correct specification of trophoectoderm from the morula stage. PMID: 25512302
    18. Intestinal nuclear bile acid farnesoid X receptor is regulated transcriptionally by CDX2. PMID: 25138215
    19. Increased expression of Cdx2 leads to an increased number of apical nuclei and subsequent symmetrical cell division, whereas down-regulation of Cdx2 leads to more nuclei moving basally, and asymmetric division. PMID: 24855000
    20. Cdx2 expression is repressed in the dorsal posterior neuropores of Pax3-null embryos. PMID: 24284205
    21. Hyperosmolar stress caused rapid loss of nuclear Cdx2 phospho ser60 and Id2 in the two-cell stage embryo. PMID: 23316940
    22. the downregulation of HNF4alpha is an important determinant of the reduced expression of the Cdx2 tumor suppressor gene in intestinal cancers. PMID: 22986531
    23. Data indicate that the H19 could induce the Cdx2 even under suppressive stress by overexpressed Oct3/4. PMID: 23743205
    24. Cdx2 transcripts preferentially localize apically at the late eight-cell stage and become inherited asymmetrically during divisions that set apart pluripotent and differentiating cells. PMID: 23375373
    25. Cdx2 cannot substitute for Cdx1 in this autoregulatory loop. This is in marked contrast with the ability of Cdx2 to support Cdx1 expression and function in paraxial mesoderm and vertebral patterning. PMID: 23382958
    26. s propose that mutually exclusive binding between Cdx2 and Oct4 in Xist underlies the switch between imprinted and random XCI in the early mouse embryo. PMID: 22942124
    27. CDX2-driven leukemogenesis involves KLF4 repression and deregulated PPARgamma signaling. PMID: 23202735
    28. CDX2 is necessary for HNF4A to bind intestinal epithelial cell DNA at cooccupied regions. CDX2 loss adversely affects the active chromatin configuration at sites of HNF4A cooccupancy. PMID: 23129810
    29. CDX2 is a tumor suppressor in colorectal cancer PMID: 23112155
    30. Cdx genes are required for the full development of the allantois and its derivatives in the placental labyrinth PMID: 22960234
    31. maternal Cdx2 is not essential for mouse development PMID: 22992952
    32. Results suggest that the main factor responsible for upregulation of Cdx2 expression in outer blastomeres is their polarity. PMID: 22609553
    33. Cdx2 was involved in regulating pro-gression of human gastric cancer cells MGC-803. PMID: 22563170
    34. Wnt, through Cdx2, activates large intestinal gene expression at high doses and small intestinal gene expression at lower doses. PMID: 21854845
    35. The interaction between CDX2 transcription factor and DDX5 RNA helicase PMID: 22359951
    36. BRA was necessary for and preceded CDX2 expression. PMID: 21816365
    37. Expression of Cdx2 was essential for differentiation of gut stem cells into any of the intestinal cell types, but they maintained a degree of cell-autonomous plasticity that allowed them to switch on a variety of gastric genes. PMID: 22190642
    38. Cdx2 is ectopically induced in the early metaplastic condition of Barrett's esophagus, its expression is not necessarily present in progressive Barrett's with dysplasia or adenocarcinoma PMID: 21935353
    39. Compound mutants carrying one active Cdx2 allele in the Cdx4-null background (Cdx2/4), transgenic embryos precociously expressing Hox13 genes and a novel Wnt3a hypomorph mutant all manifest a comparable phenotype with similar uro-rectal defects PMID: 21752936
    40. Cdx2 expression was associated with reduced basal epithelial cell proliferation and altered cell morphology. PMID: 21494671
    41. Loss of Cdx1 and Cdx2 in adult mice enhances the effects of isolated Cdx2 deficiency on mature, postmitotic enterocytes, rapidly accelerating severe malnutrition and death and resulting in alterations in the morphology of villus enterocytes. PMID: 21402776
    42. Cdx4 expression is significantly down regulated in Cdx2(-/-) mutants suggesting previously unrecognized cross-regulatory interactions. PMID: 20933081
    43. Cdx2 increased the transcriptional activity of the Sox2 gene PMID: 21036460
    44. Cdx2 plays an important role in the expression of Claudin-2 in vivo. PMID: 20602571
    45. Overexpression of Cdx2 in the small intestine is associated with reduced post-natal growth, early epithelial maturation, alterations in crypt base organization, and changes in Paneth and goblet cell lineages. PMID: 21081128
    46. identified the minimal promoter region of CDH17 that is regulated by HNF1alpha and CDX2 transcriptional factors; Suppression of HNF1alpha and CDX2 expression by siRNA down-regulated expressions of CDH17 and cyclin D1 and the viability of HCC cells PMID: 20568120
    47. Results clearly demonstrate that neither maternal nor zygotic Cdx2 transcripts direct the initiation of ICM/TE lineage separation. PMID: 21098565
    48. Results characterized enhancer elements specifically modified in differentiating intestinal epithelial cells and found enrichment of transcription factor-binding motifs corresponding to CDX2, a critical regulator of the intestine. PMID: 21074721
    49. Cdx genes function to maintain a signaling-dependent niche for the posterior axial progenitors. PMID: 20816799
    50. Cdx2 expression is regulated by Pinin PMID: 20637749

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Caudal homeobox family
  • 组织特异性：
    In the intestine, detected in ileum and proximal and distal colon (at protein level). In adult small intestine, predominantly localized in crypt and lower villus cells of the epithelium (at protein level). Expressed in the intestine but not detected in ot
  • 数据库链接：

    KEGG: mmu:12591

    STRING: 10090.ENSMUSP00000031650

    UniGene: Mm.20358