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  4. Recombinant Mouse Histone-arginine methyltransferase CARM1 (Carm1)

Recombinant Mouse Histone-arginine methyltransferase CARM1 (Carm1)

  • 中文名称：
    Recombinant Mouse Histone-arginine methyltransferase CARM1(Carm1),Yeast
  • 货号：
    CSB-YP893004MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse Histone-arginine methyltransferase CARM1(Carm1),Yeast
  • 货号：
    CSB-EP893004MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse Histone-arginine methyltransferase CARM1(Carm1),Yeast
  • 货号：
    CSB-EP893004MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse Histone-arginine methyltransferase CARM1(Carm1),Yeast
  • 货号：
    CSB-BP893004MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse Histone-arginine methyltransferase CARM1(Carm1),Yeast
  • 货号：
    CSB-MP893004MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Carm1
  • Uniprot No.：
  • 别名：
    Carm1; Prmt4Histone-arginine methyltransferase CARM1; EC 2.1.1.319; Coactivator-associated arginine methyltransferase 1; Protein arginine N-methyltransferase 4
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-608
  • 氨基酸序列
    MAAAAATAVG PGAGSAGVAG PGGAGPCATV SVFPGARLLT IGDANGEIQR HAEQQALRLE VRAGPDAAGI ALYSHEDVCV FKCSVSRETE CSRVGRQSFI ITLGCNSVLI QFATPHDFCS FYNILKTCRG HTLERSVFSE RTEESSAVQY FQFYGYLSQQ QNMMQDYVRT GTYQRAILQN HTDFKDKIVL DVGCGSGILS FFAAQAGARK IYAVEASTMA QHAEVLVKSN NLTDRIVVIP GKVEEVSLPE QVDIIISEPM GYMLFNERML ESYLHAKKYL KPSGNMFPTI GDVHLAPFTD EQLYMEQFTK ANFWYQPSFH GVDLSALRGA AVDEYFRQPV VDTFDIRILM AKSVKYTVNF LEAKEGDLHR IEIPFKFHML HSGLVHGLAF WFDVAFIGSI MTVWLSTAPT EPLTHWYQVR CLFQSPLFAK AGDTLSGTCL LIANKRQSYD ISIVAQVDQT GSKSSNLLDL KNPFFRYTGT TPSPPPGSHY TSPSENMWNT GSTYNLSSGV AVAGMPTAYD LSSVIAGGSS VGHNNLIPLA NTGIVNHTHS RMGSIMSTGI VQGSSGAQGG GGSSSAHYAV NNQFTMGGPA ISMASPMSIP TNTMHYGS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activates transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs.
  • 基因功能参考文献：
    1. C9orf72 acts by promoting the lysosomal degradation of coactivator-associated arginine methyltransferase 1 (CARM1), which in turn regulates autophagy-lysosomal functions and lipid metabolism. PMID: 30366907
    2. Study describes peptide-based transition state mimics that form stable complexes with CARM1 resulting in high-resolution co-crystal structures. The findings provide an exciting approach to understanding protein arginine methyltransferase (PRMT) substrate recognition and the regulation of arginine methylation. PMID: 28330993
    3. CARM1 promotes EZH2-mediated silencing of EZH2/BAF155 target tumor suppressor genes by methylating BAF155. PMID: 29434212
    4. AMPK deficiency results in nuclear CARM1 decrease mediated in part by SKP2, contributing to autophagy dysfunction in the aged heart. PMID: 28315332
    5. Study identifies CARM1, which methylates histone H3 at arginine 26 (H3R26), as an upstream regulator of Sox21 expression. These results indicate that heterogeneity in gene expression patterns biases cell fate in the mouse embryo as early as the 4-cell stage. PMID: 27015307
    6. CARM1 haploinsufficiency impairs transdifferentiation and wound healing in a mouse model. PMID: 25906418
    7. PRMT4 might be a key regulator of high-glucose-induced insulin secretion from pancreatic beta cells via H3R17 methylation. PMID: 25917831
    8. Data show that the methyltransferase CARM1 (coactivator-associated arginine methyltransferase 1; PRMT4) methylated Notch intracellular domain (NICD) at five conserved arginine residues. PMID: 25805888
    9. Arginine methylation of Pax7 by Carm1 functions as a molecular switch controlling the epigenetic induction of Myf5 during satellite stem cell asymmetric division and entry into the myogenic program. PMID: 22863532
    10. CARM1 is a key epigenetic regulator of hematopoiesis that affects multiple lineages at various stages of differentiation. PMID: 23248263
    11. our study provides evidence for a role of CARM1-mediated arginine methylation in regulation of histone acetylation and transcription. PMID: 22723830
    12. PRMT4 is necessary for selective control of a specific gene expression program associated with glycogen metabolism. PMID: 22428544
    13. Substantial quantities of soluble full-length mouse CARM1 were purified from transiently transfected HEK293T cells. PMID: 21126607
    14. The results demonstrate for the first time that CARM1 inhibits pulmonary cell proliferation and is required for proper differentiation of alveolar cells. PMID: 20530543
    15. Histone H3 arginine methylation by CARM1 in ES cells allows epigenetic modulation of pluripotency. PMID: 19544422
    16. CARM1 requires its enzymatic activity for all of its known cellular functions. PMID: 19897492
    17. CARM1 plays a significant role in promoting the differentiation of early thymocyte progenitors, possibly through its direct action on TARPP. PMID: 15096520
    18. results show that CARM1/PRMT4 is a novel transcriptional coactivator of NF-kappaB and functions as a promoter-specific regulator of NF-kappaB recruitment to chromatin PMID: 15616592
    19. p/CIP/SRC-3 activity and stability are regulated by CARM1-dependent methylation PMID: 17043108
    20. The identification of splicing factors that are methylated by CARM1, and protein-protein interactions that are regulated by CARM1, strongly implicates this enzyme in the regulation of alternative splicing. PMID: 17218272
    21. phosphorylation of CARM1 serves as a unique mechanism for inactivating CARM1-regulated estrogen-dependent gene expression PMID: 17640894
    22. biochemical analysis of CARM1 methyltransferase and the histone code PMID: 17882261
    23. the N-terminal and the C-terminal end of CARM1 catalytic module contain molecular switches that may determine how CARM1 regulates its biological activities by protein-protein interactions PMID: 17882262
    24. These results show that CARM1 promotes adipocyte differentiation by coactivating PPARgamma-mediated transcription and thus might be important in energy balance. PMID: 18188184
    25. study reveals thaat c-Fos-mediated transcriptional activation is regulated by CARM1 & by all 3 members of the p160 protein family of coactivators PMID: 18511550
    26. MYPT1 and CARM1 are substrates of OGT and function to alter OGT substrate specificity in vitro PMID: 18840611
    27. genetic complementation of carm1-/- cells with wild-type CARM1 or an enzymatic inactive mutant of CARM1 to investigate the requirement of CARM1 and its enzymatic activity for nuclear factor kappaB (NF-kappaB)-dependent gene expression PMID: 19769987
    28. phosphorylation at Ser(217) serves as a molecular switch for controlling CARM1 enzymatic activity during the cell cycle. PMID: 19843527

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, Protein arginine N-methyltransferase family
  • 组织特异性：
    Ubiquitously expressed. Within the brain, present in proliferating cells from lateral ventricular zone and dentate gyrus (at protein level).
  • 数据库链接：

    KEGG: mmu:59035

    STRING: 10090.ENSMUSP00000034703

    UniGene: Mm.178115