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  4. Recombinant Mouse Histone acetyltransferase KAT7 (Kat7)

Recombinant Mouse Histone acetyltransferase KAT7 (Kat7)

  • 中文名称：
    小鼠Kat7重组蛋白
  • 货号：
    CSB-YP725551MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Kat7重组蛋白
  • 货号：
    CSB-EP725551MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Kat7重组蛋白
  • 货号：
    CSB-EP725551MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Kat7重组蛋白
  • 货号：
    CSB-BP725551MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Kat7重组蛋白
  • 货号：
    CSB-MP725551MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Kat7
  • Uniprot No.：
  • 别名：
    Kat7; Hbo1; Myst2; Histone acetyltransferase KAT7; EC 2.3.1.48; Histone acetyltransferase binding to ORC1; Lysine acetyltransferase 7; MOZ; YBF2/SAS3; SAS2 and TIP60 protein 2; MYST-2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-613
  • 氨基酸序列
    MAIGVVKRNA GSSSDGTEDS DFSTDLEHTD SSESDGTSRR SARVTRSSAR LSQSSQDSSP VRNLPSFGTE EPAYSTRRVT RSQQQPTPVT PKKYPLRQTR SSGSETEQVV DFSDRETKNT ADHDESPPRT PTGNAPSSES DIDISSPNVS HDESIAKDMS LKDSGSDLSH RPKRRRFHES YNFNMKCPTP GCNSLGHLTG KHERHFSISG CPLYHNLSAD ECKVRAQSRD KQIEERMLSH RQDDNNRHAT RHQAPTERQL RYKEKVAELR KKRNSGLSKE QKEKYMEHRQ TYGNTREPLL ENLTSEYDLD LFRRAQARAS EDLEKLRLQG QITEGSNMIK TIAFGRYELD TWYHSPYPEE YARLGRLYMC EFCLKYMKSQ TILRRHMAKC VWKHPPGDEI YRKGSISVFE VDGKKNKIYC QNLCLLAKLF LDHKTLYYDV EPFLFYVMTE ADNTGCHLIG YFSKEKNSFL NYNVSCILTM PQYMRQGYGK MLIDFSYLLS KVEEKVGSPE RPLSDLGLIS YRSYWKEVLL RYLHNFQGKE ISIKEISQET AVNPVDIVST LQALQMLKYW KGKHLVLKRQ DLIDEWIAKE AKRSNSNKTM DPSCLKWTPP KGT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalytic subunit of histone acetyltransferase HBO1 complexes, which specifically mediate acetylation of histone H3 at 'Lys-14' (H3K14ac), thereby regulating various processes, such as gene transcription, protein ubiquitination, immune regulation, stem cell pluripotent and self-renewal maintenance and embryonic development. Some complexes also catalyze acetylation of histone H4 at 'Lys-5', 'Lys-8' and 'Lys-12' (H4K5ac, H4K8ac and H4K12ac, respectively), regulating DNA replication initiation, regulating DNA replication initiation. Specificity of the HBO1 complexes is determined by the scaffold subunit: complexes containing BRPF scaffold (BRPF1, BRD1/BRPF2 or BRPF3) direct KAT7/HBO1 specificity towards H3K14ac, while complexes containing JADE (JADE1, JADE2 and JADE3) scaffold direct KAT7/HBO1 specificity towards histone H4. H3K14ac promotes transcriptional elongation by facilitating the processivity of RNA polymerase II. Acts as a key regulator of hematopoiesis by forming a complex with BRD1/BRPF2, directing KAT7/HBO1 specificity towards H3K14ac and promoting erythroid differentiation. H3K14ac is also required for T-cell development. KAT7/HBO1-mediated acetylation facilitates two consecutive steps, licensing and activation, in DNA replication initiation: H3K14ac facilitates the activation of replication origins, and histone H4 acetylation (H4K5ac, H4K8ac and H4K12ac) facilitates chromatin loading of MCM complexes, promoting DNA replication licensing. Acts as a positive regulator of centromeric CENPA assembly: recruited to centromeres and mediates histone acetylation, thereby preventing centromere inactivation mediated by SUV39H1, possibly by increasing histone turnover/exchange. Involved in nucleotide excision repair: phosphorylation by ATR in response to ultraviolet irradiation promotes its localization to DNA damage sites, where it mediates histone acetylation to facilitate recruitment of XPC at the damaged DNA sites. Acts as an inhibitor of NF-kappa-B independently of its histone acetyltransferase activity.; Plays a central role in the maintenance of leukemia stem cells in acute myeloid leukemia (AML). Acts by mediating acetylation of histone H3 at 'Lys-14' (H3K14ac), thereby facilitating the processivity of RNA polymerase II to maintain the high expression of key genes, such as HOXA9 and HOXA10 that help to sustain the functional properties of leukemia stem cells.
  • 基因功能参考文献：
    1. a nonredundant role for KAT7 in the maintenance of Histone h 3 lusine 14 acetylation, which is intimately linked with the ability to develop a normal immune system, is reported. PMID: 27733580
    2. These results indicate that BRPF3 forms a functional tetrameric complex with HBO1 but is not required for mouse development and survival PMID: 26677226
    3. Taken together, MYST2 is a repressed growth suppressor in AML mediating reduced acetylation of histone 4 at residue 5 PMID: 26072331
    4. Brd1-mediated Hbo1 activity is crucial for efficient activation of CD8 expression via acetylation at H3K14, which serves as an epigenetic mark that promotes the recruitment of transcription machinery to the CD8 enhancers. PMID: 25519988
    5. Modulation of Fbxw15 levels was able to differentially regulate histone H3K14 acetylation and cellular proliferation by altering HBO1 levels. PMID: 23319590
    6. The Hbo1-Brd1 complex is the major H3K14 HAT required for transcriptional activation of erythroid developmental regulator genes. PMID: 21753189
    7. the primary role of HBO1 in development is that of a transcriptional activator, which is indispensable for H3K14 acetylation and for the normal expression of essential genes regulating embryonic development PMID: 21149574
    8. reveal a direct regulatory connection between p53-responsive stress signaling and Hbo1-dependent chromatin pathways PMID: 17954561
    9. FAD24 controls DNA replication by recruiting HBO1 to origins of DNA replication and is required for MCE during adipocyte differentiation. PMID: 18029353

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  • 亚细胞定位：
    Nucleus. Chromosome. Chromosome, centromere. Cytoplasm, cytosol.
  • 蛋白家族：
    MYST (SAS/MOZ) family
  • 组织特异性：
    Widely expressed in adult tissues.
  • 数据库链接：

    KEGG: mmu:217127

    STRING: 10090.ENSMUSP00000103362

    UniGene: Mm.472268