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  4. Recombinant Mouse Guanylyl cyclase-activating protein 1 (Guca1a)

Recombinant Mouse Guanylyl cyclase-activating protein 1 (Guca1a)

  • 中文名称：
    小鼠Guca1a重组蛋白
  • 货号：
    CSB-YP010044MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Guca1a重组蛋白
  • 货号：
    CSB-EP010044MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Guca1a重组蛋白
  • 货号：
    CSB-EP010044MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Guca1a重组蛋白
  • 货号：
    CSB-BP010044MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Guca1a重组蛋白
  • 货号：
    CSB-MP010044MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Guca1a
  • Uniprot No.：
  • 别名：
    Guca1a; Gcap; Gcap1; Guca1; Guanylyl cyclase-activating protein 1; GCAP 1; Guanylate cyclase activator 1A
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-202
  • 氨基酸序列
    GNIMEGKSV EELSSTECHQ WYKKFMTECP SGQLTLYEFR QFFGLKNLSP SASQYVEQMF ETFDFNKDGY IDFMEYVAAL SLVLKGKVEQ KLRWYFKLYD VDGNGCIDRD ELLTIIRAIR TINPWSDSSM SAEEFTDTVF AKIDINGDGE LSLEEFMEGV QKDQMLLDTL TRSLDLTGIV RRLQNGEHEE AGTGDLAAEA AG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Stimulates retinal guanylyl cyclase when free calcium ions concentration is low and inhibits guanylyl cyclase when free calcium ions concentration is elevated. This Ca(2+)-sensitive regulation of retinal guanylyl cyclase is a key event in recovery of the dark state of rod photoreceptors following light exposure. May be involved in cone photoreceptor light response and recovery of response in bright light.
  • 基因功能参考文献：
    1. The GCAP1 and GCAP2 binding site(s) overlaps within the kinase homology and/or dimerization domains of retinal GC1. PMID: 25616661
    2. Direct association between RD3 and GCAP1 is important for GC1 targeting. PMID: 25477517
    3. The wild type and mutants of GCAP1 displayed large differences in Ca(2+)-binding and regulation and preserved an intact secondary and tertiary structure with a significant rearrangement of the aromatic residues upon binding of Ca(2+). PMID: 24566882
    4. GCAP1 is the 'first-response' sensor protein that stimulates retinal membrane guanylyl cyclase early in the rod photoresponse. PMID: 23082185
    5. results argue that there must be a cellular mechanism that limits GCAP1 access to RetGC2 and makes RetGC1 isozyme a preferential target for the disease-causing GCAP1 mutants. PMID: 22623665
    6. Recombinant Gcap14 protein cosedimented with pure microtubules, indicating a direct binding between the two PMID: 22673506
    7. Calcium-myristoyl Tug is a new mechanism for intramolecular tuning of calcium sensitivity and target enzyme interaction for guanylyl cyclase-activating protein 1 PMID: 22383530
    8. Stimulation by GCAP increases the maximal velocity (Vmax) for retinal guanylyl cyclase (RetGC1) activation up to 20-fold in mouse photoreceptor outer segment. PMID: 21928830
    9. GCAP-activated native retinal membrane (Ret)GC1 and RetGC2 are less sensitive to inhibition by calcium ions in the presence of GCAP1 than GCAP2. PMID: 21598940
    10. Mutant GCAP1, when under normal expression control, causes both rod and cone photoreceptors to lose function and degenerate, with cone cells being more severely affected, in keeping with the human disease phenotype. PMID: 21464903
    11. GCAP1 at near normal levels supports the generation of wild-type flash responses in the absence of GCAP2. PMID: 11927539
    12. GCAP functions in intact photoreceptors [review] PMID: 12596933
    13. These results provide the first direct evidence that a mutation [in GCAP1] linked to congenital blindness increases Ca2+ in the outer segment, which may trigger the apoptotic process. PMID: 15240799
    14. Prolonged illumination up-regulates retinal arrestin and Guca1a/b: a novel mechanism for light adaptation. PMID: 19332500

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  • 亚细胞定位：
    Membrane; Lipid-anchor. Photoreceptor inner segment. Cell projection, cilium, photoreceptor outer segment. Note=Subcellular location is not affected by light or dark conditions.
  • 组织特异性：
    In the retina, expressed in rod photoreceptors (at protein level). Expressed in cone photoreceptors.
  • 数据库链接：

    KEGG: mmu:14913

    STRING: 10090.ENSMUSP00000060027

    UniGene: Mm.16224