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  4. Recombinant Mouse Guanine nucleotide-binding protein subunit beta-5 (Gnb5)

Recombinant Mouse Guanine nucleotide-binding protein subunit beta-5 (Gnb5)

  • 中文名称：
    小鼠Gnb5重组蛋白
  • 货号：
    CSB-YP009608MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Gnb5重组蛋白
  • 货号：
    CSB-EP009608MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Gnb5重组蛋白
  • 货号：
    CSB-EP009608MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Gnb5重组蛋白
  • 货号：
    CSB-BP009608MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Gnb5重组蛋白
  • 货号：
    CSB-MP009608MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Gnb5; Guanine nucleotide-binding protein subunit beta-5; Gbeta5; Transducin beta chain 5
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-395
  • 氨基酸序列
    MCDQTFLVNV FGSCDKCFKQ RALRPVFKKS QQLNYCSTCA EIMATDGLHE NETLASLKSE AESLKGKLEE ERAKLHDVEL HQVAERVEAL GQFVMKTRRT LKGHGNKVLC MDWCKDKRRI VSSSQDGKVI VWDSFTTNKE HAVTMPCTWV MACAYAPSGC AIACGGLDNK CSVYPLTFDK NENMAAKKKS VAMHTNYLSA CSFTNSDMQI LTASGDGTCA LWDVESGQLL QSFHGHGADV LCLDLAPSET GNTFVSGGCD KKAMVWDMRS GQCVQAFETH ESDVNSVRYY PSGDAFASGS DDATCRLYDL RADREVAIYS KESIIFGASS VDFSLSGRLL FAGYNDYTIN VWDVLKGSRV SILFGHENRV STLRVSPDGT AFCSGSWDHT LRVWA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Enhances GTPase-activating protein (GAP) activity of regulator of G protein signaling (RGS) proteins, hence involved in the termination of the signaling initiated by the G protein coupled receptors (GPCRs) by accelerating the GTP hydrolysis on the G-alpha subunits, thereby promoting their inactivation (Probable). Increases RGS9 GTPase-activating protein (GAP) activity, hence contributes to the deactivation of G protein signaling initiated by D(2) dopamine receptors. May play an important role in neuronal signaling, including in the parasympathetic, but not sympathetic, control of heart rate.
  • 基因功能参考文献：
    1. The complex of G protein regulator RGS9-2 and Gbeta(5) controls sensitization and signaling kinetics of type 5 adenylyl cyclase in the striatum. PMID: 22932702
    2. Expression of the Gbeta5/R7-RGS protein complex in pituitary and pancreatic islet cells. PMID: 22322946
    3. Gbeta5/RGS6 (guanine nucleotide binding protein beta5/regulator of G-protein signaling 6) complex modulates timing of parasympathetic influence on atrial myocytes and heart rate. PMID: 20884879
    4. Elimination of Gbeta5-RGS complexes led to a striking level of hyperactivity that far exceeds activity levels previously observed in animal models. PMID: 21766168
    5. We conclude that even a partial reduction in Gbeta5-R7 level can perturb normal animal metabolism and behavior. PMID: 21804131
    6. Gbeta5 regulates dendritic arborization and/or synapse formation during development, in part by effects on gene expression; mice lacking Gbeta5 have a markedly abnormal neurologic phenotype. PMID: 21883221
    7. regulator of G-protein signalling 7 binding protein contributes significantly to the nuclear localization of endogenous G beta(5)/R7-RGS complex in brain PMID: 20100282
    8. study found that RGS7-Gbeta5 complexes bound to G protein-gated potassium channels and facilitated their functional coupling to GABA(B) receptors in neurons PMID: 20453851
    9. Disruption of the Gbeta5 gene in mice abolishes expression of Gbeta5-L in the retina and also greatly reduces the expression level of RGS9-1, indicating a complex containing RGS9-1-Gbeta5-L is essential for normal G-protein deactivation and rod function PMID: 12904457
    10. photoreceptors R9AP determines the stability of the RGS9.Gbeta5 complex, and therefore all three proteins, RGS9, Gbeta5 , and R9AP, are obligate members of the regulatory complex that speeds the rate at which transducin hydrolyzes GTP. PMID: 14625292
    11. G-protein beta5 short splice variant isoform is indispensable for outer plexiform layer integrity and normal light responses of the retina. PMID: 18094259
    12. R7-Gbeta5-R7BP complexes in the mouse could regulate signaling by modulatory Gi/o-coupled GPCRs in the developing and adult nervous systems. PMID: 18248908
    13. Delivery of RGS7/Gbeta5 to dendrites of ON-bipolar cells occurs independently of its association with R7BP. These findings provide a new mechanism for adapter-independent targeting of RGS/Gbeta5 complexes. PMID: 18842904
    14. Gbeta5 and R7BP in striatum undergo remodeling upon changes in neuronal activity. PMID: 19332565

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  • 亚细胞定位：
    Membrane.
  • 蛋白家族：
    WD repeat G protein beta family
  • 组织特异性：
    Isoform 1 is only detected in retina. Isoform 2 is detected in brain (at protein level). Isoform 2 is detected in brain.
  • 数据库链接：

    KEGG: mmu:14697

    STRING: 10090.ENSMUSP00000076155

    UniGene: Mm.17604