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  4. Recombinant Mouse Guanine nucleotide-binding protein G (t) subunit alpha-1 (Gnat1)

Recombinant Mouse Guanine nucleotide-binding protein G (t) subunit alpha-1 (Gnat1)

  • 中文名称：
    小鼠Gnat1重组蛋白
  • 货号：
    CSB-YP009598MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Gnat1重组蛋白
  • 货号：
    CSB-EP009598MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Gnat1重组蛋白
  • 货号：
    CSB-EP009598MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Gnat1重组蛋白
  • 货号：
    CSB-BP009598MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Gnat1重组蛋白
  • 货号：
    CSB-MP009598MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Gnat1
  • Uniprot No.：
  • 别名：
    Gnat1; Gnat-1Guanine nucleotide-binding protein G(t) subunit alpha-1; Transducin alpha-1 chain
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-350
  • 氨基酸序列
    GAGASAEEK HSRELEKKLK EDAEKDARTV KLLLLGAGES GKSTIVKQMK IIHQDGYSLE ECLEFIAIIY GNTLQSILAI VRAMTTLNIQ YGDSARQDDA RKLMHMADTI EEGTMPKEMS DIIQRLWKDS GIQACFDRAS EYQLNDSAGY YLSDLERLVT PGYVPTEQDV LRSRVKTTGI IETQFSFKDL NFRMFDVGGQ RSERKKWIHC FEGVTCIIFI AALSAYDMVL VEDDEVNRMH ESLHLFNSIC NHRYFATTSI VLFLNKKDVF SEKIKKAHLS ICFPDYDGPN TYEDAGNYIK VQFLELNMRR DVKEIYSHMT CATDTQNVKF VFDAVTDIII KENLKDCGLF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Functions as signal transducer for the rod photoreceptor RHO. Required for normal RHO-mediated light perception by the retina. Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs), such as the photoreceptor RHO. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Activated RHO promotes GDP release and GTP binding. Signaling is mediated via downstream effector proteins, such as cGMP-phosphodiesterase.
  • 基因功能参考文献：
    1. GNAT1 role in phototransduction of rod photoreceptors PMID: 28320964
    2. Data suggest light reduces oxygen consumption rate (OCR) of retina; light does not affect OCR in retinas of Gnat1-/- knockout mice (phototransduction disabled). Light reduces cGMP in retina; light does not alter cGMP in Gnat1-/- plus Gnat2-/- retina. PMID: 26677218
    3. Biochemical changes, including reduced transducin protein levels and enhanced transducin GTPase activity, explain the shift in light intensity threshold for Galphat1 translocation in Rs1-knockout mice. PMID: 24664744
    4. The results from shaker1 and whirler mice suggest that defective transducin translocation may be functionally related to light-induced degeneration. PMID: 24211856
    5. Oligomerization incompetent retinal degeneration slow is associated with mislocalization of cone opsins and cone transducin. PMID: 22183390
    6. Thus our results showed that IRD1 and IRD2 mice harbor a nonsense mutation in the Gnat1 gene, resulting in the absence or suppressed expression of the Tralpha protein, which is the likely cause of rod dysfunction in both mutants. PMID: 19766629
    7. alpha-transducin can function in taste receptor cells and transduce some taste cell responses PMID: 12379596
    8. data suggest that phosducin action is based on the reduction of transducin affinity to the membranes of rod outer segments, achieved by keeping the transducin beta gamma subunits apart from the alpha subunit. PMID: 14973130
    9. Alpha-transducin played no role in regulating adaptive response to light. Degeneration does not require transducin-mediated signaling. PMID: 15180267
    10. Alpha-transducin played no role in responses to salty, bitter, & sweet compounds or to inosine monophoshate. It was involved in the umami taste of MSG & monopotassium glutamate. Umami detection by alpha-transducin occurs in anteriorly placed taste buds. PMID: 15342734
    11. The localization and interaction with Gtalpha suggest LGN roles in modulation of transducin translocation and other photoreceptor cell functions. PMID: 15737739
    12. the light-dependent translocations of transducin are controlled by the GTP-hydrolysis on Gtalpha, and apparently, do not require Gtalpha interaction with RGS9 and PDE6 PMID: 16207703
    13. The light-dependency threshold for transducin translocation is determined by the balance between the rate of transducin dissociation from membranes and the capacity of the GTPase-activating complex. PMID: 17267570
    14. Phosducin does not contribute to adaptation mechanisms of the outer segment by dynamically controlling heterotrimer availability, but is necessary for maintaining normal transducin expression and therefore normal flash sensitivity in rods. PMID: 17724163
    15. The gamma-subunit controls the expression level of the entire transducin heterotrimer and that heterotrimer formation is essential for normal transducin localization. PMID: 18367617
    16. Transducin deletion blocked the apoptotic signaling induced by exposure to low light conditions, and it did not require c-Fos/AP-1. PMID: 18985007
    17. rhodopsin palmitylation has a role in activation of transducin PMID: 19348429

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  • 亚细胞定位：
    Cell projection, cilium, photoreceptor outer segment. Membrane; Peripheral membrane protein. Photoreceptor inner segment.
  • 蛋白家族：
    G-alpha family, G(i/o/t/z) subfamily
  • 组织特异性：
    In the retina, expressed in the rod photoreceptors.
  • 数据库链接：

    KEGG: mmu:14685

    STRING: 10090.ENSMUSP00000010205

    UniGene: Mm.284853