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  4. Recombinant Mouse Glycine receptor subunit alpha-3 (Glra3), partial

Recombinant Mouse Glycine receptor subunit alpha-3 (Glra3), partial

  • 中文名称：
    小鼠Glra3重组蛋白
  • 货号：
    CSB-YP852798MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Glra3重组蛋白
  • 货号：
    CSB-EP852798MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Glra3重组蛋白
  • 货号：
    CSB-EP852798MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Glra3重组蛋白
  • 货号：
    CSB-BP852798MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Glra3重组蛋白
  • 货号：
    CSB-MP852798MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Glra3Glycine receptor subunit alpha-3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Glycine receptors are ligand-gated chloride channels. Channel opening is triggered by extracellular glycine. Channel characteristics depend on the subunit composition; heteropentameric channels display faster channel closure. Plays an important role in the down-regulation of neuronal excitability. Contributes to the generation of inhibitory postsynaptic currents. Contributes to increased pain perception in response to increased prostaglandin E2 levels. Plays a role in the regulation of breathing rhythm, especially of the duration of the postinspiratory phase. Plays a role in cellular responses to ethanol.
  • 基因功能参考文献：
    1. This experiments identify the alpha3 subunit of the glycine receptor as a target of modulation in a mouse model of Rett syndrome. PMID: 29203410
    2. alpha2 and alpha3 GlyRs are present in various regions of the forebrain and that alpha3 GlyRs specifically participate in tonic inhibition in the striatum and prefrontal cortex. PMID: 28784756
    3. loss of alpha3-GlyRs in mice compromises suprathreshold auditory nerve activity, but not outer hair cell function PMID: 27208792
    4. GlyRalpha3 KO animals showed significantly impaired prepulse inhibition of the auditory startle response and changes in auditory brainstem response. PMID: 27891689
    5. Findings suggest that trigeminal primary afferent boutons receive presynaptic modulation via homomeric, extrasynaptic glycine receptor subunit alpha 3, and that different subtypes of glycine receptors may be involved in pre- and postsynaptic inhibition. PMID: 26832918
    6. presynaptic glycine release in the hypoglossal nucleus is partially depending on GlyR alpha3. PMID: 26851771
    7. The postsynaptic clusters of a3-containing GlyRs werelocated to efferent synapses of the medial and lateral olivocochlear bundle in the murine organ of Corti beyond the onset of hearing. PMID: 21850450
    8. Both GlyRalpha2 and GlyRalpha3 modulate local receptive fields in the retina: GlyRalpha3 mediates serial inhibition, whereas GlyRalpha2 participates in crossover inhibition. PMID: 22399754
    9. these data suggest most glycine receptors and glycinergic synapses in the superficial dorsal horn contain alpha1 subunits and few are composed exclusively of alpha3 subunits. PMID: 21486794
    10. Data show that rhythmic respiratory activity is affected by phosphorylation status of the inhibitory glycine receptor alpha3 subtype, which controls glutamatergic and glycinergic neuronal discharges, subject to serotonergic receptor 1A modulation. PMID: 20978350
    11. synapses containing GlyR alpha3 contribute in large part to synaptic inhibition in lamina II; results clearly show that presynaptic GABA/glycine release properties are influenced by the nature and complexity of postsynaptic inhibitory receptor subtypes PMID: 20092571
    12. demonstrated that inhibition of GlyR alpha3 by PGE2-induced receptor phosphorylation underlies central inflammatory pain sensitization; GlyR alpha3 is distinctly expressed in superficial layers of the spinal cord dorsal horn PMID: 15131310
    13. Spinal glycine receptor alpha3 subunit does not contribute to pain after peripheral nerve injury or formalin injection. PMID: 16846696
    14. Glycinergic sIPSCs of amacrine cells displayed medium fast kinetics (mean decay time constant tau=11+/-2 ms), which were completely absent in the Glra3-/- mouse. PMID: 17920294

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  • 亚细胞定位：
    Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell junction, synapse. Perikaryon. Cell projection, dendrite. Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Ligand-gated ion channel (TC 1.A.9) family, Glycine receptor (TC 1.A.9.3) subfamily, GLRA3 sub-subfamily
  • 组织特异性：
    Detected in brainstem, also in neurons that control rhythmic breathing. Detected in superficial laminae of the dorsal horn of the thoracic spinal cord. Detected in dentate gyrus in hippocampus, especially in stratum granulare. Detected in the inner plexif
  • 数据库链接：

    KEGG: mmu:110304

    STRING: 10090.ENSMUSP00000000275

    UniGene: Mm.307061