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  4. Recombinant Mouse Glutamate decarboxylase 2 (Gad2)

Recombinant Mouse Glutamate decarboxylase 2 (Gad2)

  • 中文名称：
    小鼠Gad2重组蛋白
  • 货号：
    CSB-YP009160MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Gad2重组蛋白
  • 货号：
    CSB-EP009160MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Gad2重组蛋白
  • 货号：
    CSB-BP009160MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Gad2重组蛋白
  • 货号：
    CSB-MP009160MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Gad2
  • Uniprot No.：
  • 别名：
    Gad2; Gad65Glutamate decarboxylase 2; EC 4.1.1.15; 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 65 kDa isoform
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-585
  • 氨基酸序列
    MASPGSGFWS FGSEDGSADP ENPGTARAWC QVAQKFTGGI GNKLCALLYG DSGKPAEGGG SVTSRAATGK VACTCDQKPC NCPKGDVNYA FLHATDLLPA CDGERPTLAF LQDVMNILLQ YVVKSFDRST KVIDFHYPNE LLQEYNWELA DQPQNLEEIL THCQTTLKYA IKTGHPRYFN QLSTGLDMVG LAADWLTSTA NTNMFTYEIA PVFVLLEYVT LKKMREIIGW PGGSGDGIFS PGGAISNMYA MLIARYKMFP EVKEKGMAAV PRLIAFTSEH SHFSLKKGAA ALGIGTDSVI LIKCDERGKM IPSDLERRIL EVKQKGFVPF LVSATAGTTV YGAFDPLLAV ADICKKYKIW MHVDAAWGGG LLMSRKHKWK LSGVERANSV TWNPHKMMGV PLQCSALLVR EEGLMQSCNQ MHASYLFQQD KHYDLSYDTG DKALQCGRHV DVFKLWLMWR AKGTTGFEAH IDKCLELAEY LYTIIKNREG YEMVFDGKPQ HTNVCFWFVP PSLRTLEDNE ERMSRLSKVA PVIKARMMEY GTTMVSYQPL GDKVNFFRMV ISNPAATHQD IDFLIEEIER LGQDL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the production of GABA.
  • 基因功能参考文献：
    1. These results thus identify a molecularly distinct, orexin-activated lateral hypothalamus submodule that governs physical activity in mice. PMID: 28396414
    2. Increased activity due to bicuculline preferentially up-regulated GAD65. This was reduced by AP5, a selective blocker of Ca2+-permeable ion channels and NMDA-Rs, and by U0126, KN93, and K252a, affecting BDNF pathways. PMID: 26241953
    3. Data show that miRNAs are involved in regulating the expression of the major type 1 diabetes (T1D) autoantigens IA-2, IA-2beta, and GAD65 enzyme. PMID: 26148972
    4. Results show that GAD65 is required for efficient GABAergic synaptic transmission and plasticity, and for maintaining extracellular GABA at a level needed for associative plasticity at cortical inputs in the lateral amygdala PMID: 24663011
    5. Menin regulates spinal glutamate-GABA balance through GAD65 contributing to neuropathic pain. PMID: 24905306
    6. Our data suggest a role of GAD65-mediated GABA synthesis in the encoding of circadian information to fear memory PMID: 24300892
    7. Autoimmunity to GAD65 may play a role in the development of Stiff person syndrome. PMID: 24058450
    8. Prenatal and postnatal maternal effects influence GAD65 and GAD67 gene expression depending on the age of the offspring. PMID: 23876745
    9. These findings indicate novel, differential roles for GluN2A, B and D receptors and for GAD65-mediated GABA in the regulation of individual topographies of orofacial movement PMID: 23892010
    10. SMAR1 and p53 act synergistically to up-regulate GAD65 expression upon Streptozotocin treatment. PMID: 22978699
    11. Data show that chronic pain epigenetically suppresses Gad2 transcription through histone deacetylase-mediated histone hypoacetylation, resulting in impaired gamma-aminobutyric acid synaptic inhibition. PMID: 21983856
    12. These results suggest that modulation of the synaptic transmission from primary afferents to SG neurons by group III mGluR agonist is specific to the type of nociceptive primary afferents but not to the type of target neurons. PMID: 21177998
    13. GAD65 is crucial for maintenance of biosynthesis of synaptic GABA particularly by direct synthesis from astrocytic glutamine via glutamate. PMID: 20664610
    14. An impaired tonic inhibition in GAD65-deficient mice is revealed demonstrating a significant role of GAD65 in the synthesis of GABA acting extrasynaptically. PMID: 21039523
    15. Mice possessing a monoclonal GAD65-specific CD4-positive T cell population develop a lethal encephalomyelitis-like disease in the absence of any other T cells or B cells. PMID: 20348424
    16. Deletion of Gad2 reduced the effect of flurazepam on motor incoordination and increased the effect of extrasynaptic GABAA receptor agonist gabaxadol without changing the duration of loss of righting reflex produced by these drugs. PMID: 20002022
    17. GAD65 plays an important role in regulating fear responses during extinction of cued but apparently not contextual fear. PMID: 20016086
    18. Absence of long-term depression in the visual cortex of GAD65 knock-out mice PMID: 12097476
    19. CD4+ T cells from glutamic acid decarboxylase (GAD)65-specific T cell receptor transgenic mice are not diabetogenic and can delay diabetes transfer PMID: 12186840
    20. Induction of autoimmune diabetes through insulin (but not this enzyme)DNA vaccination in NOD mice PMID: 12401715
    21. No significant GAD65 gene expression differences are detected in either drug-naive or acute ethanol withdrawn mouse brain. PMID: 12691782
    22. Results indicate that glutamic acid decarboxylase (GAD65) is not an essential autoantigen in the pathogenesis of diabetes. PMID: 12796471
    23. Selectively altered behavioral fear response in GAD65 deficient mice, most likely due to deficits in threat estimation or elicitation of appropriate conditioned fear behavior. GAD65 is a genetic determinant of conditioned fear behavior. PMID: 12884963
    24. The cumulative incidence of autoimmune diabetes was found not to be influenced by GAD65 deficiency in NOD mice. PMID: 14676944
    25. These results suggest that GAD65-generated GABA is implicated more complex processing of taste information including taste mixtures and palatability may be finely tuned by GAD65-mediated GABA synthesis. PMID: 15036622
    26. GAD65 knockout mice showed robust deficits in prepulse inhibition which were reversed by the atypical antipsychotic agent clozapine PMID: 15114343
    27. Similar to the rat at P5, mice at P6 are unable to sustain an increase in CO2 induced respiratory frequency and GAD65 contributes to this fall off. PMID: 15134589
    28. P boutons can be distinguished from other GABAergic terminals in the ventral horn of rat and mouse spinal cord by their high level of the glutamic acid decarboxylase (GAD) 65 isoform of GAD. PMID: 15947074
    29. lack of gad65 impairs the function of the respiratory network in mouse fetuses and the impairment progresses with fetal age PMID: 17418495
    30. GAD65 plays a major role in GABA transmission in normal physiological condition. PMID: 17482148
    31. GAD65-mediated GABA synthesis is critical for the consolidation of stimulus-specific fear memory PMID: 18323571
    32. anti-whole GAD65-reactive T cells have the ability to regulate the development of type 1 diabetes PMID: 19188044
    33. There are reduced NMDA NR2A levels and slower NMDA currents in visual cortex of GAD65 knockout mice PMID: 19406876
    34. study found that the number of somatic GAD65-puncta on individual layer 2/3 pyramidal neurons is reduced when mice are dark-reared from birth and reverted to normal levels by re-exposure to light PMID: 19503840
    35. GAD65-mediated gamma-aminobutyric acid (GABA) synthesis plays relatively small but significant roles in nociceptive processing via supraspinal mechanisms. PMID: 19571163
    36. MHC class I-restricted responses to GAD65 provide an inflammatory focus for the generation of islet-specific pathogenesis and beta cell destruction, as well as a therapeutic role for GAD65 peptide determinants in type I diabetes. PMID: 11466400

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  • 亚细胞定位：
    Cytoplasm, cytosol. Cytoplasmic vesicle. Cell junction, synapse, presynaptic cell membrane; Lipid-anchor. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side.
  • 蛋白家族：
    Group II decarboxylase family
  • 数据库链接：

    KEGG: mmu:14417

    STRING: 10090.ENSMUSP00000028123

    UniGene: Mm.4784