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  4. Recombinant Mouse Glucose-6-phosphate isomerase (Gpi)

Recombinant Mouse Glucose-6-phosphate isomerase (Gpi)

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  • 中文名称：
    小鼠Gpi重组蛋白
  • 货号：
    CSB-YP009717MO
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • The purity of Gpi was greater than 95% as determined by SEC-HPLC
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
    Greater than 95% as determined by SEC-HPLC.
  • 基因名：
    Gpi
  • Uniprot No.：
  • 别名：
    Gpi; Gpi1Glucose-6-phosphate isomerase; GPI; EC 5.3.1.9; Autocrine motility factor; AMF; Neuroleukin; NLK; Phosphoglucose isomerase; PGI; Phosphohexose isomerase; PHI
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    Yeast
  • 分子量：
    64.6kDa
  • 表达区域：
    2-558aa
  • 氨基酸序列
    AALTRNPQFQKLLEWHRANSANLKLRELFEADPERFNNFSLNLNTNHGHILVDYSKNLVNKEVMQMLVELAKSRGVEAARDNMFSGSKINYTENRAVLHVALRNRSNTPIKVDGKDVMPEVNRVLDKMKSFCQRVRSGDWKGYTGKSITDIINIGIGGSDLGPLMVTEALKPYSKGGPRVWFVSNIDGTHIAKTLASLSPETSLFIIASKTFTTQETITNAETAKEWFLEAAKDPSAVAKHFVALSTNTAKVKEFGIDPQNMFEFWDWVGGRYSLWSAIGLSIALHVGFDHFEQLLSGAHWMDQHFLKTPLEKNAPVLLALLGIWYINCYGCETHALLPYDQYMHRFAAYFQQGDMESNGKYITKSGARVDHQTGPIVWGEPGTNGQHAFYQLIHQGTKMIPCDFLIPVQTQHPIRKGLHHKILLANFLAQTEALMKGKLPEEARKELQAAGKSPEDLEKLLPHKVFEGNRPTNSIVFTKLTPFILGALIAMYEHKIFVQGIMWDINSFDQWGVELGKQLAKKIEPELEGSSAVTSHDSSTNGLISFIKQQRDTKLE
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    A construct encoding NH2-terminally 6xHis-tagged mouse glucose-6-phosphate isomerase (Gpi) was expressed in the yeast cells. The resulting protein is the recombinant full-length of mature Gpi protein containing amino acid sequence 2-558 of mouse Gpi. The purity of this Gpi protein is greater than 90% determined by SDS-PAGE. A molecular mass band range from 60 kDa to 66 kDa was presented on the gel under reducing conditions. In-stock Gpi proteins are offered now. This recombinant Gpi protein may be used to produce anti-Gpi antibodies or in the studies of glycolysis, gluconeogenesis, or Gpi-related disorders.

    Gpi is a glycolytic enzyme that catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate, the second reaction step of glycolysis. It is also involved in gluconeogenesis and the pentose phosphate pathway. Besides, Gpi can be secreted to the extracellular matrix functioning like a cytokine or growth factor. Studies demonstrated that Gpi acts as a neurotrophic factor that promotes the survival of skeletal motor neurons and sensory neurons and as a lymphokine that stimulates the secretion of immunoglobulin. Gpi deficiency causes nonspherocytic hemolytic anemia and is even related to fetal edema, immediate neonatal death, and neurological dysfunction.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis. Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility. Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons. It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion.
  • 基因功能参考文献：
    1. PGI is a moonlighting protein that functions as a cytosolic enzyme involved in glycolysis and gluconeogenesis, and as cytokine through binding to its cell surface receptor. PMID: 8674049
    2. The mGPI-transgenic mice presented GPI more efficiently and showed a dramatic increase in negative selection and an inhibition of arthritis. PMID: 23840022
    3. Data show that silencing of AMF/PGI induced MET and suppressed the tumor growth and pulmonary metastasis of LM8 cells in nude mice. PMID: 20978190
    4. induces hyperpermeability of endothelial and mesothelial cells leading to accumulation of ascites fluid (tumor autocrine motility factor) PMID: 12054583
    5. acid-induced conformational changes in phosphoglucose isomerase result in its increased cell surface association and deposition on fibronectin fibrils PMID: 12888574
    6. Gpi1 immunoreactivity was found in ganglion, amacrine, horizontal and bipolar cells, but not in rods, cones, pigment epithelium and Muller cells. PMID: 15182299
    7. High-resolution crystal structures of the enzyme from mouse have been determined in native form and in complex with the inhibitor erythrose 4-phosphate, and with the substrate glucose 6-phosphate. PMID: 15342241
    8. crystal structure of Autocrine Motility Factor PMID: 15502335
    9. AMF expression significantly contributes to the aggressive phenotype of pancreatic cancer PMID: 15570012
    10. Results report the first high-resolution crystal structure of the inhibitor-free form and the eight types of inhibitor complexes of mouse autocrine motility factor. PMID: 16375918
    11. the receptor molecule for AMF/NLK/MF in leukemic differentiation is not gp78 PMID: 17071500

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  • 亚细胞定位：
    Cytoplasm. Secreted.
  • 蛋白家族：
    GPI family
  • 数据库链接：

    KEGG: mmu:14751

    STRING: 10090.ENSMUSP00000049355

    UniGene: Mm.589