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  4. Recombinant Mouse Gamma-aminobutyric acid receptor subunit gamma-2 (Gabrg2), partial

Recombinant Mouse Gamma-aminobutyric acid receptor subunit gamma-2 (Gabrg2), partial

  • 中文名称：
    小鼠Gabrg2重组蛋白
  • 货号：
    CSB-YP009152MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Gabrg2重组蛋白
  • 货号：
    CSB-EP009152MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Gabrg2重组蛋白
  • 货号：
    CSB-EP009152MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Gabrg2重组蛋白
  • 货号：
    CSB-BP009152MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Gabrg2重组蛋白
  • 货号：
    CSB-MP009152MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Gabrg2
  • Uniprot No.：
  • 别名：
    Gabrg2Gamma-aminobutyric acid receptor subunit gamma-2; GABA(A) receptor subunit gamma-2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Ligand-gated chloride channel which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain. Plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel. The gamma2 subunit is necessary but not sufficient for a rapid formation of active synaptic contacts and the synaptogenic effect of this subunit is influenced by the type of alpha and beta subunits present in the receptor pentamer. The alpha1/beta2/gamma2 receptor, alpha2/beta2/gamma2 receptor and the alpha1/beta3/gamma2 receptor exhibit synaptogenic activity whereas the alpha2/beta3/gamma2 receptor shows very little or no synaptogenic activity. Functions also as histamine receptor and mediates cellular responses to histamine.
  • 基因功能参考文献：
    1. This study found that the inactivation of gamma2-mediated synapses does not impact oligodendrocyte precursors proliferation and differentiation or the propensity of oligodendrocyte precursors to myelinate their presynaptic interneurons. PMID: 28795438
    2. The results of this study suggesting that mutations like GABRG2(Q390X) may alter brain thermal regulation and precipitate seizures during temperature elevations. PMID: 28505490
    3. This study demonstrated that the reduced local input to fast-spiking interneurons in the somatosensory cortex in the GABAA gamma2 R43Q mouse model of absence epilepsy. PMID: 28195311
    4. Heterozygous Gabrg2(+/Q390X) KI mice are associated with a severe epileptic encephalopathy due to a dominant negative effect of the mutation, while heterozygous Gabrg2(+/-) KO mice are associated with mild absence epilepsy due to simple haploinsufficiency. PMID: 27340224
    5. This investigation demonstrates striking interfacial GABAA receptor subunit selectivity in the native milieu, suggesting that asymmetric occupancy of heteropentameric ion channels by alkylphenol-based anesthetics is sufficient to induce modulation of activity. PMID: 27462076
    6. This study describes a novel mechanism that regulates plasma receptor pools in the control of synaptic receptor density. PMID: 25891999
    7. Neurotoxins from Snake Venoms and alpha-Conotoxin ImI Inhibit Functionally Active Ionotropic gamma-Aminobutyric Acid (GABA) Receptors PMID: 26221036
    8. Study shows that the down-regulation of the gamma2 subunit of postsynaptic GABAARs in NG2 cells that accompanies the postnatal switch from synaptic to extrasynaptic GABAergic transmission between interneurons and these cells in the barrel cortex PMID: 24217990
    9. Dendritic spine abnormalities of Gabrg2+/- mice suggest association of functional defects in glutamatergic transmission with the established anxious-depressive phenotype of these mice PMID: 25107590
    10. results suggest that the g2(R43Q) mutation significantly affects cortical microcircuitry in the cerebral cortex. PMID: 25740860
    11. KIF21B participates in the delivery of GABAAR transport vesicles into dendrites. PMID: 25172774
    12. The gamma2 subunits (of GABA A receptors) cell surface levels were decreased by mutating each of the Beta2 subunit glycosylation sites. PMID: 24213971
    13. These results indicate that thalamic deltaGABAARs promote electrocortical signatures of deep NREM sleep, but do not mediate the effects of etomidate at the thalamus in vivo. PMID: 25209268
    14. The data of this study demonstrate the presence of IPSCs and the synaptic enrichment of the alpha1 and beta3 subunits and suggest that the gamma3 subunit is the most likely candidate for clustering GABAARs at synapses in the absence of the gamma2 subunit. PMID: 25080584
    15. The study demonstrates the molecular and functional diversity of the GABAAR system within the mouse colon providing a framework for developing GABAAR-based therapeutics in GI disorders. PMID: 25080596
    16. This study showed that oscillatory activity in thalamic circuits is under the control of inhibition that involves gamma2 subunit-independent GABA-ARs PMID: 24849349
    17. Tyrosine phosphorylation of GABAA receptor gamma2-subunit regulates tonic and phasic inhibition in the Lateral Geniculate Body in mice. PMID: 23904608
    18. Expression of the gamma2-subunit distinguishes synaptic and extrasynaptic GABA(A) receptors in NG2 cells of the hippocampus PMID: 23864689
    19. Our results suggest a model whereby a temporary decrease in GABA level would modulate the filtering parameters of the corticostriatal circuit, allowing a more robust induction of high frequency-dependent plasticity. PMID: 23069672
    20. A single gene mutation in GABA gamma2 can cause distinct seizure phenotypes through independent molecular mechanisms. PMID: 23408872
    21. The results suggested that juvenile and adult stressor experiences elicit variations of GABAA receptor subunit expression that are region-specific as well as sexually-dimorphic. Stressful events during the juvenile. PMID: 22542673
    22. This study demonistrated that Gabrg2 gene expression in mouse dorsal raphe nucleus PMID: 22534482
    23. phenotype of mice with GABA(A) receptor gamma2 subunits from parvalbumin neurons PMID: 21912668
    24. Findings show that 70-90% reductions in the normal levels of GABA(A) receptor activity at the GnRH neuron appear to impact upon the estrogen negative feedback mechanism but are, nevertheless, compatible with normal fertility in mice. PMID: 20573723
    25. Modest deficits in GABAergic synaptic transmission in mice that are heterozygous for the Gabrg2 gene lead to behavior, and endocrine and pharmacologic alterations reminiscent of melancholic major depression PMID: 20579975
    26. The Gabrg2 gene is essential for benzodiazepine modulation of GABAergic inhibitory neurotransmission in vivo. PMID: 7644489
    27. The g2 subunit is essential for postsynaptic clustering of GABA(A) receptors and gephyrin, and for GABAergic synaptic transmission PMID: 7644489
    28. Hypothalamic somatostatin and growth hormone-releasing hormone mRNA expression depend upon GABA(A) receptor expression in the developing mouse. PMID: 12169770
    29. Nova regulates GABA(A) receptor gamma2 alternative splicing via a distal downstream UCAU-rich intronic splicing enhancer PMID: 12808107
    30. The GABA(A) receptor gamma 2 subunit is required for synaptic localiation and clustering of GABA(A) receptors not only during development but also at mature synapses. PMID: 14572465
    31. Mice with a point mutation in this gene show an abolition in zolpidem sensitivity. PMID: 15165831
    32. GODZ-mediated palmitoylation is a novel posttranslational modification that is selective for gamma-subunit-containing GABA(A) receptor subtypes, a mechanism that may be important for regulated trafficking of these receptors in the secretory pathway. PMID: 15229235
    33. A developmental sequence of postsynaptic events is proposed in which diffuse extrasynaptic Gabrg2 subunits accumulate at inhibitory synapses to form postsynaptic clusters, mostly colocalizing with glycine receptor clusters in the adult. PMID: 15610161
    34. Subunit binding is perturbed when the R43Q mutation is present suggesting that this residue is critical for the formation of inter-subunit contacts at (+) interfaces of GABAA subunits PMID: 15866052
    35. gephyrin is required for the membrane insertion/stabilization of the GABAAR gamma2 subunit as well as for its subsequent localization in the postsynaptic membrane PMID: 15963957
    36. Thus, the gamma2F77I point mutation virtually abolished the actions of zolpidem on GABA(A) receptors in the hippocampus. However, some residual effects of zolpidem may involve receptors that do not contain the gamma2 subunit. PMID: 15978011
    37. Results from this study, together with recent knockout studies, point to the GABA(A) receptor gamma2 subunit gene as a promising candidate gene underlying phenotypic differences in sedative-hypnotic physiological dependence and withdrawal episodes. PMID: 16436183
    38. histamine modulates heteromultimeric GABA(A) receptors and may thus represent an endogenous ligand for an allosteric site PMID: 18281286
    39. A significant decrease in phasic inhibition occurs in pregnant mice, mediated by a downregulation of GABA(A) receptor gamma 2 subunit, which rebounds immediately postpartum. PMID: 18667149

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  • 亚细胞定位：
    Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Cell projection, dendrite. Cytoplasmic vesicle membrane.
  • 蛋白家族：
    Ligand-gated ion channel (TC 1.A.9) family, Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily, GABRG2 sub-subfamily
  • 组织特异性：
    Expressed in brain neurons (at protein level).
  • 数据库链接：

    KEGG: mmu:14406

    STRING: 10090.ENSMUSP00000063812

    UniGene: Mm.5309