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  4. Recombinant Mouse GTP-binding protein Rheb (Rheb)

Recombinant Mouse GTP-binding protein Rheb (Rheb)

  • 中文名称：
    小鼠Rheb重组蛋白
  • 货号：
    CSB-YP835633MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Rheb重组蛋白
  • 货号：
    CSB-EP835633MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Rheb重组蛋白
  • 货号：
    CSB-EP835633MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Rheb重组蛋白
  • 货号：
    CSB-BP835633MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Rheb重组蛋白
  • 货号：
    CSB-MP835633MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Rheb; GTP-binding protein Rheb; Ras homolog enriched in brain
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-181
  • 氨基酸序列
    MPQSKSRKIA ILGYRSVGKS SLTIQFVEGQ FVDSYDPTIE NTFTKLITVN GQEYHLQLVD TAGQDEYSIF PQTYSIDING YILVYSVTSI KSFEVIKVIH GKLLDMVGKV QIPIMLVGNK KDLHMERVIS YEEGKALAES WNAAFLESSA KENQTAVDVF KRIILEAEKI DGAASQGKSS C
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Activates the protein kinase activity of mTORC1, and thereby plays a role in the regulation of apoptosis. Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Has low intrinsic GTPase activity.
  • 基因功能参考文献：
    1. This study found that cRheb expression promoted regeneration of injured rVRG axons into the lesion site, while we observed no rVRG axon regrowth with AAV2-GFP control. PMID: 29453976
    2. Rheb1 is critical for the polarization of macrophages and inhibition of allergic asthma. Deletion of Rheb1 enhances M2 polarization but decreases M1 polarization in alveolar macrophages, leading to the aggravation of OVA-induced allergic asthma. PMID: 28225024
    3. Ggpps deletion enhanced Rheb farnesylation, which subsequently activated mTORC1 and facilitated spermatogonial differentiation PMID: 27374985
    4. Thus, the various regulatory elements that impinge upstream of mTORC1 activation pathways are differentially required for HSC homeostasis in vivo. PMID: 29175333
    5. Forebrain depletion of Rheb GTPase elicits spatial memory deficits. PMID: 27960107
    6. Rheb is an important negative regulator of beige fat development and thermogenesis. Rheb is able to suppress the beiging effect through an mTORC1-independent mechanism, via PDE4D5-dependent downregulation of the cAMP-PKA signaling pathway. PMID: 28242620
    7. Rheb and TSC2 have roles in the mechanical activation of mTOR signaling PMID: 28289099
    8. Rheb1 is critical for macrophage production and phagocytosis and executes these activities possibly via mTORC1-dependent pathway. PMID: 27163399
    9. EAAT4 was downregulated due to the loss of Rheb1 in Purkinje cells; mTORC1 was downregulated and Akt was upregulated in Rheb1 cKO mice, suggesting that mTORC1 and Akt may be related to the downregulation of EAAT4; Rheb1 knockout decreased EAAT4 currents and slowed down the kinetics of AMPA currents; Rheb1 deficiency did not affect the morphology of Purkinje cell layer and the development of Purkinje cells PMID: 26194056
    10. The expression pattern of RHEB1 suggests that it likely plays a ubiquitous role in the development of the early embryo with more tissue-specific roles in later development. PMID: 26708151
    11. Data suggest that RAS-homolog enriched in brain protein (Rheb1) promotes MLL-AF9 fusion protein initiated acute myeloid leukemia (AML) progression through target of rapamycin complex 1 (mTORC1) signaling pathway. PMID: 27071307
    12. Rheb activation disrupts neuronal spine synapse formation via syntenin accumulation in tuberous sclerosis complex. PMID: 25880340
    13. We conclude that in contrast to TORC1 hyper-activity, cognitive function is not very sensitive to sustained and specific down-regulation of TORC1 activity. PMID: 25759467
    14. Rheb protein was mainly detected in apoptotic retinal ganglion cells following light injury. PMID: 24664437
    15. PDK4 promotes tumorigenesis through activation of the CREB-RHEB-mTORC1 signaling cascade. PMID: 25164809
    16. We conclude that oligodendrocyte progenitor cells-intrinsic mTORC1 activity mediated by Rheb1 is critical for differentiation of oligodendrocyte progenitor cells PMID: 25411504
    17. The small GTPase Rheb is required for spermatogenesis but not oogenesis. PMID: 24713393
    18. The paper proposed a novel pathway/mechanism involving Rheb/mTORC1/S6K signaling to explain how C2-ceramide impairs insulin signaling via Akt phosphorylation. PMID: 24650522
    19. whereas rapid adaptation in mTORC1 activity requires Rheb, a second Rheb-independent activation mechanism exists that contributes to cell cycle progression. PMID: 24303063
    20. deletion of Rheb1 in the brain causes a reduction in mTORC1 activity and impairs food intake. PMID: 24451134
    21. miR-155 bound to the 3'-untranslated region of Ras homologue enriched in brain (Rheb), a negative regulator of autophagy, accelerated the process of autophagy and sequential killing of intracellular mycobacteria by suppressing Rheb expression. PMID: 24130493
    22. study defines Rheb as a novel physiological regulator of BACE1 levels and Abeta generation, and the Rheb-BACE1 circuitry may have a role in brain biology and disease. PMID: 24368770
    23. Rheb (Ras homologue enriched in brain)-dependent mammalian target of rapamycin complex 1 (mTORC1) activation becomes indispensable for cardiac hypertrophic growth after early postnatal period PMID: 23426372
    24. Newborn Rheb-expressing neurons display altered distribution in olfactory bulb. PMID: 23392671
    25. Opposing functions of Rheb Tyr35 in the intrinsic and GAP-stimulated GTP catalysis are critical for optimal mTORC1 regulation. PMID: 22819219
    26. Treatment with FTS decreased Ras.GTP only slightly in the TSC2-null cells, but reduced their overactive Rheb as well as their proliferation, migration and tumor growth. PMID: 21500191
    27. Inactivation of Rheb protects cardiac myocytes during energy deprivation through activation of autophagy. PMID: 22294621
    28. Redox regulates mammalian target of rapamycin complex 1 (mTORC1) activity by modulating the TSC1/TSC2-Rheb GTPase pathway. PMID: 21784859
    29. in NOD mice, a model of spontaneous autoimmune T1D, Rheb overexpression significantly accelerated diabetes progression. PMID: 21513702
    30. Rheb(-/-) embryonic fibroblasts showed decreased TORC1 activity, were smaller, and showed impaired proliferation PMID: 21321084
    31. Rheb1 is essential for mTORC1 signaling and myelination in the brain PMID: 21238928
    32. results suggest a novel regulatory mechanism for mTORC1 in which the cAMP-determined dynamic interaction between Rheb and PDE4D provides a key, unique regulatory event, and propose a new role for PDE4 as a molecular transducer for cAMP signaling. PMID: 20837708
    33. integrity of late endosomes is essential for amino acid- and insulin-stimulated mTORC1 signaling and blocking the early/late endosomal conversion prevents mTOR from interacting with Rheb in the late endosomal compartment PMID: 20053679
    34. RHEB is an MTOR activator that facilitates multistage carcinogenesis through induction of multiple oncogenic mechanisms PMID: 20388784
    35. It activates mTorc1. (review) PMID: 20058701
    36. ERas and Rheb are two Ras-related proteins involved in the phosphatidylinositol 3-kinase/mTOR pathway; ERas is localized at the plasma membrane, while Rheb is on endomembranes. PMID: 16046393
    37. ATF6alpha-Rheb-mTOR signaling promotes survival of dormant tumor cells in vivo PMID: 18650380
    38. Inhibition of PI3K or mTOR reduced the level of Rap1B, which acts downstream of Rheb and mTOR. The ubiquitin E3 ligase Smurf2 mediates the restriction of Rap1B by initiating its degradation. PMID: 18842593
    39. This study reveals a function of Rheb in controlling misfolded protein metabolism by modulating aggresome formation. PMID: 19458266

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  • 亚细胞定位：
    Endomembrane system; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytosol. Endoplasmic reticulum membrane; Lipid-anchor; Cytoplasmic side.
  • 蛋白家族：
    Small GTPase superfamily, Rheb family
  • 数据库链接：

    KEGG: mmu:19744

    STRING: 10090.ENSMUSP00000030787

    UniGene: Mm.319175