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  4. Recombinant Mouse G-protein-signaling modulator 2 (Gpsm2)

Recombinant Mouse G-protein-signaling modulator 2 (Gpsm2)

  • 中文名称：
    小鼠Gpsm2重组蛋白
  • 货号：
    CSB-YP837717MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Gpsm2重组蛋白
  • 货号：
    CSB-EP837717MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Gpsm2重组蛋白
  • 货号：
    CSB-EP837717MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Gpsm2重组蛋白
  • 货号：
    CSB-BP837717MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Gpsm2重组蛋白
  • 货号：
    CSB-MP837717MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Gpsm2
  • Uniprot No.：
  • 别名：
    Gpsm2; Lgn; PinsG-protein-signaling modulator 2; Pins homolog
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-679
  • 氨基酸序列
    MEGNLISMRE DHSFHVRYRM EASCLELALE GERLCKSGDC RAGVSFFEAA VQVGTEDLKT LSAIYSQLGN AYFYLHDYAK ALEYHHHDLT LARTIGDQLG EAKASGNLGN TLKVLGNFDE AIVCCQRHLD ISRELNDKVG EARALYNLGN VYHAKGKSFG CPGPQDTGEF PEDVRNALQA AVDLYEENLS LVTALGDRAA QGRAFGNLGN THYLLGNFRD AVIAHEQRLL IAKEFGDKAA ERRAYSNLGN AYIFLGEFET ASEYYKKTLL LARQLKDRAV EAQSCYSLGN TYTLLQDYEK AIDYHLKHLA IAQELKDRIG EGRACWSLGN AYTALGNHDQ AMHFAEKHLE ISREVGDKSG ELTARLNLSD LQMVLGLSYS TNNSMMSENI EIDGSLHGAG AKLGRRHSME NLELMKLTPE KVPNWNSEIL AKQKPLIAKP SAKLLFVNRL KGKKYKSGSA CTKVLQDASN SVDHRAPRSQ KKISSDTIGD EGFFDLLRRF QSNRMDDQRC HLQGNCRTTS TAAASATPKL MKAPSVSVVS PNTDEFLDLL ASSQSRRLDD QRASFSNLPG LRLTKGNSPS VLERLMTNDK KEPDEDFFDI LVKCQGSRLD DQRCAPPSAA TKGPTVPDED FFSLILRSQA KRMDEQRVLL QRDPNRDSEF GLKELLQNNA LLEFKHSGK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays an important role in mitotic spindle pole organization via its interaction with NUMA1. Required for cortical dynein-dynactin complex recruitment during metaphase. Plays a role in metaphase spindle orientation. Plays an important role in asymmetric cell divisions. Has guanine nucleotide dissociation inhibitor (GDI) activity towards G(i) alpha proteins, such as GNAI1 and GNAI3, and thereby regulates their activity.
  • 基因功能参考文献：
    1. Mutation of the mouse orthologue of GPSM2 affects actin-rich stereocilia elongation in auditory and vestibular hair cells, causing deafness and balance defects. Gpsm2 defines an approximately 200 nm nanodomain at the tips of stereocilia and this localization requires the presence of Galphai3, myosin 15 and whirlin. Loss of Gpsm2 leads to decreased outgrowth and a disruption of actin dynamics in neuronal growth cones. PMID: 28387217
    2. LGN in complex with the asymmetric domain of Inscuteable, which reveals a tetrameric arrangement of intertwined molecules. PMID: 29523789
    3. LGN and Galphai participate in a long-inferred signal that originates outside the bundle to model its staircase-like architecture, a property that is essential for direction sensitivity to mechanical deflection and hearing. PMID: 27660326
    4. Kinocilium is essential for proper localization of Lgn, as well as Gai and aPKC, suggesting that cilium function plays a role in positioning of apical proteins critical for hearing. PMID: 26662512
    5. these results show a context-dependent function for LGN. PMID: 26755700
    6. support for the hitherto untested model that Par3-mInsc and Galphai3 act cooperatively to polarize LGN and promote perpendicular divisions PMID: 25016959
    7. LGN-TPR motifs are versatile and capable of recognizing multiple targets via diverse binding modes. PMID: 23318951
    8. X-ray crystallography and binding studies reveal that LGN GoLoco binding motifs are potent guanine nucleotide dissociation inhibitors. PMID: 22952234
    9. The restricted localization of NuMA in the lateral belt is instructive for the planar alignment of the mitotic spindle, and required for its planar maintenance. PMID: 21444683
    10. Knocking out LGN, (the G protein regulator), randomized the orientation of normally planar neuroepithelial divisions. PMID: 18084280
    11. The primary aim of this study was to determine the spatial distribution and time course of mouse homolog of Drosophila Partner of Inscuteable (mPins) expression in the developing and adult mouse eye. PMID: 19122831
    12. Excessive LGN induces meiotic spindle organization defects by elongating the spindle and enhancing pole focusing, whereas depletion of LGN by RNA interference results in meiotic spindle deformation and chromosome misalignment. PMID: 19434098

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  • 亚细胞定位：
    Cytoplasm. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton, spindle pole. Lateral cell membrane.
  • 蛋白家族：
    GPSM family
  • 组织特异性：
    Detected in brain and liver (at protein level). Detected in brain, spleen, liver and testis, and at lower levels in heart, lung and kidney. Enriched in the ventricular zone of the developing central nervous systems. Expressed in proximal colon, ileum, ova
  • 数据库链接：

    KEGG: mmu:76123

    STRING: 10090.ENSMUSP00000029482

    UniGene: Mm.226941