Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse Fibronectin type III domain-containing protein 5 (Fndc5), partial

Recombinant Mouse Fibronectin type III domain-containing protein 5 (Fndc5), partial

  • 货号：
    CSB-YP815686MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP815686MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP815686MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP815686MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Fndc5
  • Uniprot No.：
  • 别名：
    Fndc5; Frcp2; PepFibronectin type III domain-containing protein 5; Fibronectin type III repeat-containing protein 2; Peroxisomal protein; PeP) [Cleaved into: Irisin]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    mediates beneficial effects of muscular exercise. Induces browning of white adipose tissue by stimulating UCP1 expression, at least in part, via the nuclear receptor PPARA.
  • 基因功能参考文献：
    1. A high-fat diet increased Fndc5 transcript levels in the skeletal muscle, but exercise had a minimal effect on the transcript level of Fndc5, whereas endurance training increased the protein content of FNDC5 in the skeletal muscle. PMID: 29365291
    2. These findings may lead to development of an Irisin-based therapy for elderly immobile osteoporotic and physically disable patients, and might represent a countermeasure for astronauts subjected to microgravity-induced bone and muscle losses. PMID: 28588307
    3. Glucocorticoid receptor positively regulates transcription of FNDC5 in the liver. PMID: 28240298
    4. Findings demonstrated that one bout of both uphill and downhill exercise trainings as well as 8 weeks of training could increase the expression of PGC-1alpha and FNDC5 genes in the muscle tissues and the UCP1 gene in the subcutaneous adipose tissue. PMID: 30218749
    5. Our study has indicated that irisin (FNDC5) possesses important anti-oxidant and anti-inflammatory properties and may protect cells from the damage induced by ROS under inflammatory conditions by activating of the Nrf2/HO-1 pathway. PMID: 29769428
    6. FNDC5 attenuated Oxidized low density lipoprotein-induced AMPK deactivation, hepatic stellate cells activation, connective tissue growth factor and transforming growth factor-beta upregulation and extracellular matrix deposition in mouse hepatic stellate cells. PMID: 30007970
    7. the coordinated expression of FNDC5 and PGC-1alpha may contribute to the increased levels of plasma irisin after exercise. PMID: 29415899
    8. Irisin(Fndc5) increases myogenic differentiation and myoblast fusion via activation of IL6 signaling. PMID: 29062100
    9. FNDC5 is overexpressed in skeletal muscle and adipose tissue of insulin resistant high fat-fed mice. PMID: 28676551
    10. This study for the first time showed that adipocytes are directly affected by irisin (Fndc5) and provides an evidence on anti-inflammatory action of irisin on fat cells. PMID: 28614774
    11. Mstn regulates Fndc5/Irisin expression and secretion through a novel miR-34a-dependent post-transcriptional mechanism; loss of Mstn in mice leads to the increased Fndc5/Irisin expression, which contributes to the browning of white adipocytes PMID: 27297797
    12. The secretion of FNDC5 from myotubes and beta-cells in response to exogenous fatty acids, the effects of recombinant FNDC5 on insulin biosynthesis and glucose-stimulated insulin secretion, and beta-cell apoptosis are reported. PMID: 28724742
    13. Irisin is upregulated in a murine model of fibrosis, but not in experimental NAFLD without significant fibrosis. PMID: 28472477
    14. Irisin improved endothelial function by modulating HO-1/ adiponectin axis in perivascular adipose tissue (PVAT) in HFD-induced obese mice. These findings suggest that regulating PVAT function may be a potential mechanism by which irisin improves endothelial function in obesity. PMID: 28595178
    15. The results indicate that FNDC5 deficiency impairs autophagy and FAO and enhances lipogenesis via the AMPK/mTOR pathway. FNDC5 deficiency aggravates whereas FNDC5 overexpression prevents the HFD-induced hyperlipemia, hepatic lipid accumulation, and impaired FAO and autophagy in the liver. PMID: 27504012
    16. This research is the first to show that irisin modulates macrophage activity by reducing reactive oxygen species (ROS) overproduction, which could suggest its potential anti-inflammatory properties. PMID: 28315350
    17. No FNDC5 expression was detected in normal or cancerous stomach tissues. FNDC5 expression in white and brown adipose tissues in the cancer group increased compared with the control and non-cancer groups. PMID: 27256459
    18. Report showed that irisin suppressed cholesterol synthesis in hepatocytes through the activation of 5' AMP-activated protein kinase (AMPK) and subsequent inhibition of transcription and nuclear translocation of SREBP2. PMID: 27211556
    19. PGC1alpha regulates FNDC5 and its processed and secreted peptide Irisin, which has been proposed to play a critical role in energy expenditure and to promote neural differentiation of mouse embryonic stem cells. Review. PMID: 26611102
    20. Irisin directly targets osteoblast, promoting osteoblast proliferation and differentiation via activating P38/ERK MAP kinase signaling cascades in vitro. PMID: 26738434
    21. irisin does not function through inflammatory NFKB activation like other myokines (such as TNFalpha). PMID: 26399516
    22. Muscle FNDC5 mRNA expression and irisin release are not IL-15-dependent in mice. PMID: 25920499
    23. irisin alleviates endothelial dysfunction in type 2 diabetes partially via reducing oxidative stresses through inhibiting signaling pathways suggesting that irisin may be a promising molecule for the treatment of vascular complications of diabetes. PMID: 26225842
    24. HFD induced obese mice showed decreased irisin secretion from adipose tissues, which might contribute to muscle insulin resistance. PMID: 26261526
    25. Our study also suggests the existence of irisin-specific receptor on the membrane of H9C2 cells. In conclusion, irisin in a certain concentration rage increased myocardial cell metabolism, inhibited cell proliferation and promoted cell differentiation PMID: 26305684
    26. In summary, these results suggested that the endothelium-dependent relaxation of irisin is mediated by the nitric oxide (NO)-guanosine 3', 5'-cyclic phosphate (cGMP)-dependent pathway. PMID: 26582714
    27. Fndc5 facilitates neural differentiation. This effect might be related to increased expression of BDNF following overexpression of Fndc5. PMID: 25572300
    28. This study used irisin radiolabeled with (125)I and small-animal SPECT/CT imaging to investigate the metabolic elimination and distribution of irisin in vivo. PMID: 25757030
    29. High intensity exercise results in increased expression of Fndc5 in skeletal muscle. PMID: 26166638
    30. Increase PGC-1alpha expression during cardiac precursor cells formation stage via rosiglitazone treatment increase FNDC5 and mitochondrial markers transcript levels which enhanced cardiac differentiation efficiency. PMID: 25936576
    31. The myokine irisin increases cortical bone mass and may be the molecular entity responsible for muscle-bone connectivity. PMID: 26374841
    32. findings shed light on the poorly understood regulation of irisin/FNDC5 by demonstrating a novel association between irisin and SMAD3 signaling in skeletal muscle PMID: 25648888
    33. Stage dependent expression of FNDC5 is affected by neural induction method used for neural differentiation. PMID: 24706335
    34. Data suggest that fibronectin type III domain-containing 5 protein (Fndc5) may be involved in cardiomyocyte differentiation. PMID: 25168892
    35. exists in muscle and serum of mice independent of exercise and is increased immediately after acute exercise PMID: 24644429
    36. FNDC5 (25 kDa) and irisin (12 kDa) were present in murine skeletal muscle and that irisin was circulating in murine serum. PMID: 24498244
    37. These findings link endurance exercise and the important metabolic mediators, PGC-1alpha and FNDC5, with BDNF expression in the brain. PMID: 24120943
    38. This immunohistochemical results demonstrate the expression of irisin immunoreactivity in three types of cells: skeletal, cardiac muscle, and Purkinje cells of the cerebellum. PMID: 23470775
    39. Fndc5 expression is required for the appropriate neural differentiation of mESCs. These data confirm the importance of Fndc5 in the generation and development of the nervous system. PMID: 23219938
    40. Within the muscle, Mstn(-/-) leads to increased expression of AMPK and its phosphorylation, which subsequently activates PGC1alpha and Fndc5. PMID: 23362117

    显示更多

    收起更多

  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Peroxisome membrane; Single-pass type I membrane protein. Secreted. Note=Imported in peroxisomes through the PEX5 receptor pathway.; [Irisin]: Secreted. Note=Detected in the blood of individuals subjected to endurance exercise.
  • 组织特异性：
    In adult, it is highly expressed in skeletal muscle, heart and brain.
  • 数据库链接：

    KEGG: mmu:384061

    STRING: 10090.ENSMUSP00000099660

    UniGene: Mm.44075