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  4. Recombinant Mouse Fermitin family homolog 2 (Fermt2)

Recombinant Mouse Fermitin family homolog 2 (Fermt2)

  • 货号：
    CSB-YP809298MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP809298MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP809298MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP809298MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP809298MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Fermt2
  • Uniprot No.：
  • 别名：
    Fermt2; Plekhc1Fermitin family homolog 2; Kindlin-2; Pleckstrin homology domain-containing family C member 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-680
  • 氨基酸序列
    MALDGIRMPD GCYADGTWEL SVHVTDLNRD VTLRVTGEVH IGGVMLKLVE KLDVKKDWSD HALWWEKKRT WLLKTHWTLD KCGIQADAKL QFTPQHKLLR LQLPNMKYVK VKVNFSDRVF KAVSDICKTF NIRHPEELSL LKKPRDPTKK KKKKLDDQSE DEALELEGPL IMPGSGSIYS SPGLYSKTMT PTYDAHDGSP LSPTSAWFGD SALSEGNPGI LAVSQPVTSP EILAKMFKPQ ALLDKAKTNQ GWLDSSRSLM EQDVKENEAL LLRFKYYSFF DLNPKYDAIR INQLYEQAKW ALLLEEIECT EEEMMMFAAL QYHINKLSIM TSENHLNNSD KEVDEVDAAL SDLEITLEGG KTSTILGDIT SIPELADYIK VFKPKKLTLK GYKQYWCTFK DTSISCYKSR EESSGTPAHQ LNLRGCEVTP DVNISGQKFN IKLLIPVAEG MNEIWLRCDN EKQYAHWMAA CRLASKGKTM ADSSYNLEVQ NILSFLKMQH LNPDPQLIPD QITTDVNPEC LVSPRYLKKY KSKQITARIL EAHQNVAQMS LIEAKMRFIQ AWQSLPEFGI THFIARFQGG KREELIGIAY NRLIRMDAST GDAIKTWRFS NMKQWNVNWE IKMVTVEFAD EVRLSFICTE VDCKVVHEFI GGYIFLSTRA KDQNESLDEE MFYKLTSGWV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Scaffolding protein that enhances integrin activation mediated by TLN1 and/or TLN2, but activates integrins only weakly by itself. Binds to membranes enriched in phosphoinositides. Enhances integrin-mediated cell adhesion onto the extracellular matrix and cell spreading; this requires both its ability to interact with integrins and with phospholipid membranes. Required for the assembly of focal adhesions. Participates in the connection between extracellular matrix adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits FBLIM1 to focal adhesions. Plays a role in the TGFB1 and integrin signaling pathways. Stabilizes active CTNNB1 and plays a role in the regulation of transcription mediated by CTNNB1 and TCF7L2/TCF4 and in Wnt signaling.
  • 基因功能参考文献：
    1. In a mouse model of wound healing, KINDLIN-2 gene knockdown inhibited wound healing, and increased neovascular permeability in vivo. PMID: 30070977
    2. Our studies identify Kindlin-2 as a stabilizing linker of endothelial AJs with the actin cytoskeleton, thereby re-enforcing the vascular barrier. PMID: 28799653
    3. Kindlin-2 is a key protein that couples cell adhesion by activating integrins and the induction of membrane protrusions by activating Rac1 and supplying Rac1 with the Arp2/3 complex. PMID: 28912124
    4. Postnatal loss of Kindlin-2 from cardiac myocytes leads to progressive heart failure. PMID: 27502369
    5. Data indicate that Kindlin-2 not only maintains the cardiac structure but also is required for cardiac function. PMID: 27722852
    6. These findings show that talin and kindlin cooperatively activate integrins leading to fibronectin binding and adhesion. PMID: 26821125
    7. These studies uncover a previously unrecognized function for Kindlin-2 and a mechanism for regulation of the chondrocyte differentiation programme and chondrogenesis. PMID: 26151572
    8. Taken together, our study suggests the suppressive roles of Kindlin-2 in the pathogenesis of colorectal carcinoma PMID: 25633062
    9. Data suggest that kindlin-2 (Kind2/Fermt2) interacts with actin alpha 2 (Actn2) and integrin beta 1 (Itgb1) and co-localizes to cardiac sarcomere at Z-disc; knockdown of Kind2 leads to dissociation of Actn2 and Itgb1. PMID: 26143257
    10. beta3/kindlin-2 interaction promotes outside-in alphaVbeta3 signaling selectively, with biological consequences in vivo [kindlin-2] PMID: 25587038
    11. kindlin-2 tyrosine phosphorylation and interaction with Src serve as a regulatable switch downstream of focal adhesion kinase in the integrin outside-in signaling circuit controlling cell migration and proliferation PMID: 25237194
    12. kindlin-2, through activating Ras and the downstream ERK1/2 and Akt signaling pathways, plays an important role in regulating renal tubular epithelial-to-mesenchymal transition PMID: 24226523
    13. kindlin-2 regulates trafficking of endothelial cell surface enzymes that control platelet responses and hemostasis. PMID: 23896409
    14. Kindlin-2 mediates activation of TGF-beta/Smad signaling and renal fibrosis. PMID: 23723426
    15. Kindlin has a role in regulating integrin alpha5beta1 surface expression. PMID: 22795696
    16. Mediation of Rac1 activation by kindlin-2: an essential function in osteoblast adhesion, spreading, and proliferation PMID: 21590706
    17. Endothelial cells derived from kindlin-2(+/-) mice had integrin expression levels similar to WT mice but reduced alphaVbeta3-dependent signaling, migration, adhesion, spreading, and tube formation. PMID: 21378273
    18. physiological role for kindlin-2 in skin fibroblasts under normal steady-state conditions and during tissue regeneration PMID: 20861856
    19. kindlin-2 is an essential component of the intercalated disc, is necessary for cytoskeletal organization at sites of membrane attachment, and is required for vertebrate myocardial formation and function PMID: 18174465
    20. Kindlin-2 is a novel and essential element of bidirectional integrin signaling. PMID: 18483218
    21. kindlin-2 is a novel integrin adaptor protein important for muscle differentiation, and is a critical regulator of myocyte elongation. PMID: 18611274

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  • 亚细胞定位：
    Cytoplasm. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, stress fiber. Cell junction, focal adhesion. Membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus. Cytoplasm, myofibril, sarcomere, I band. Cell surface.
  • 蛋白家族：
    Kindlin family
  • 组织特异性：
    Detected in adult heart muscle (at protein level). Detected in heart, skeletal muscle and testis.
  • 数据库链接：

    KEGG: mmu:218952

    STRING: 10090.ENSMUSP00000044554

    UniGene: Mm.210018