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  4. Recombinant Mouse F-box/WD repeat-containing protein 8 (Fbxw8)

Recombinant Mouse F-box/WD repeat-containing protein 8 (Fbxw8)

  • 中文名称：
    小鼠Fbxw8重组蛋白
  • 货号：
    CSB-YP806383MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Fbxw8重组蛋白
  • 货号：
    CSB-EP806383MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Fbxw8重组蛋白
  • 货号：
    CSB-EP806383MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Fbxw8重组蛋白
  • 货号：
    CSB-BP806383MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Fbxw8重组蛋白
  • 货号：
    CSB-MP806383MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Fbxw8F-box/WD repeat-containing protein 8; F-box and WD-40 domain-containing protein 8
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-598
  • 氨基酸序列
    MDDHNLEEFR RHWQEELAQS QALRRRRRLE AGERRSPRRP EAGARGEPAS GYLGLAQGLL EGAGRPPAPR PGRGGDRKDT SSRSRSPPDR DATEPEPLVD QLIRDLNELD DVPFFDVRLP YELAINIFQY LNRRELGLCA QVSKTWKVIA EDEVLWYRLC RQEGHLPHSR FSDYTCWKLI LQECLAKEHT LRANWKNRKG AVSELEHVPD AVLCDVRSHD GVVIAGYTSG DVRVWDTRTW DYVAPFLESE SEEEDPGMQP YVSFVRINSS LAVAAYEDGI LNIWDLRTGR FPIFRFEHDA RIQALALSQE KPIVATASAF DVVMLYPNEE GHWHVASEFE VQKLVDYLEI VPNTGRYPVA IATAGDLVYL LKADDSARTL HYVYGQPATC LDVSASQVAF GVKSLGWVYE GNKILVYSLE AERCLSKLGN ALGDFTCVNI RDSPPNLMVS GNMDRRVRIH DLRSDKIALS LSAHQLGVSA VQMDDWKVVS GGEEGLVSVW DYRMNQKLWE VHSRHPVRYL SFNSHSLITA NVPYEKVLRN SDLDNFACHR RHRGLIHAYE FAVDQLAFQS PLPVCRLPRD IMAGYSYDLA LSFPHDSI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Substrate-recognition component of a Cul7-RING ubiquitin-protein ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. The Cul7-RING(FBXW8) complex mediates ubiquitination and consequent degradation of GORASP1, acting as a component of the ubiquitin ligase pathway that regulates Golgi morphogenesis and dendrite patterning in brain. The Cul7-RING(FBXW8) complex also mediates ubiquitination of MAP4K1/HPK1: recognizes and binds autophosphorylated MAP4K1/HPK1, leading to its degradation, thereby affecting cell proliferation and differentiation. Associated component of the 3M complex, suggesting that it mediates some of 3M complex functions.
  • 基因功能参考文献：
    1. FBXW8 and PARK2 are sequestrated into insolubility by ATXN2 PolyQ expansions, but only FBXW8 expression is dysregulated PMID: 25790475
    2. mTORC2 negatively feeds back to IRS-1 via control of Fbw8 stability and localization. PMID: 23142081
    3. our data support the concept that expanded ATXN2 undergoes progressive insolubility and affects PABPC1 by a toxic gain-of-function mechanism with tissue-specific effects, which may be partially alleviated by the induction of FBXW8. PMID: 22956915
    4. Depletion of FBXW8 caused a significant accumulation of cyclin D1, as well as sequestration of CDK1 in the cytoplasm. This resulted in a severe reduction of cell proliferation. PMID: 17205132
    5. Cul7 forms a heterodimeric complex with Cul1 in a manner dependent on Fbxw8. PMID: 16880526
    6. FBXW8-CUL7 complex plays a significant role in growth control PMID: 17998335

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  • 亚细胞定位：
    Cytoplasm, perinuclear region. Golgi apparatus.
  • 组织特异性：
    Widely expressed. Expressed at higher level in skeletal muscle, cartilage and lung.
  • 数据库链接：

    KEGG: mmu:231672

    STRING: 10090.ENSMUSP00000047012

    UniGene: Mm.221769