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  4. Recombinant Mouse F-box/LRR-repeat protein 3 (Fbxl3)

Recombinant Mouse F-box/LRR-repeat protein 3 (Fbxl3)

  • 货号：
    CSB-YP807464MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP807464MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP807464MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP807464MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP807464MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Fbxl3
  • Uniprot No.：
  • 别名：
    Fbxl3; Afh; Fbl3a; Fbxl3a; OvtmF-box/LRR-repeat protein 3; F-box and leucine-rich repeat protein 3A; F-box/LRR-repeat protein 3A; Protein after-hours; Protein overtime
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-428
  • 氨基酸序列
    MKRGGRDSDQ DSAEEGTAEK PKRPRTTQER SQPCDWGNLL QDIVLHVFKY LPLLDRAHAS QVCRNWNQVF HMPDLWRCFE FELNQPATSY LKATHPELIK QIIKRHSNHL QYVSFKVDSS KESAEAACDI LSQLVNCSLK TLGLISTARP SFMDLPKSHF ISALTVVFVN SKSLSSLKID DTPVDDPSLK VLVANNSDTL KLLKMSSCPH VSPAGILCVA DQCHGLRELA LNYHLLSDEL LLALSSEKHV RLEHLRIDVV SENPGQTHFH TIQKSSWDAF IKHSPKVNLV MYFFLYEEEF DPFFRYEIPA THLYFGRSVS KDVLGRVGMT CPRLVELVVC ANGLRPLDEE LIRIAERCKN LSAIGLGECE VSCSAFVEFV KMCGGRLSQL SIMEEVLIPD QKYSLEQIHW EVSKHLGRVW FPDMMPTW
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Substrate-recognition component of the SCF(FBXL3) E3 ubiquitin ligase complex involved in circadian rhythm function. Plays a key role in the maintenance of both the speed and the robustness of the circadian clock oscillation. The SCF(FBXL3) complex mainly acts in the nucleus and mediates ubiquitination and subsequent degradation of CRY1 and CRY2. Activity of the SCF(FBXL3) complex is counteracted by the SCF(FBXL21) complex.
  • 基因功能参考文献：
    1. CRY2 and FBXL3 cooperatively degrade c-MYC preventing the development of cancer. PMID: 27840026
    2. By analyzing the Fbxl3 and Cryptochrome 1 double-mutant mice, we found that FBXL3 also regulates the amplitudes of E-box-driven gene expression. PMID: 23471982
    3. crystal structures of mammalian CRY2 in its apo, FAD-bound and FBXL3-SKP1-complexed forms PMID: 23503662
    4. FBXL21 plays a dual role: protecting CRY 1/2 proteins (CRY) from FBXL3 degradation in the nucleus and promoting CRY degradation within the cytoplasm. PMID: 23452855
    5. results are consistent with previous studies of mutants with extended circadian periods and suggest that disruption of FBXL3 is associated with mania-like behaviours in both mice and humans PMID: 22719873
    6. In Ovtm mice, expression of the PERIOD proteins is reduced; however, the CRY proteins are unchanged; the loss of FBXL3 function leads to a stabilization of the CRY proteins, which leads to a global transcriptional repression of the Per and Cry genes. PMID: 17462724
    7. study demonstrates that the SCF(Fbxl3)ubiquitin ligase controls the oscillations of the circadian clock by mediating the degradation of Cry1 and Cry2 proteins PMID: 17463251
    8. studies reveal a central role for Fbxl3 in mammalian circadian timekeeping PMID: 17463252

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Note=Predominantly nuclear.
  • 组织特异性：
    Ubiquitously expressed but enriched in brain. Diffusely expressed in the suprachiasmatic nucleus, SCN.
  • 数据库链接：

    KEGG: mmu:50789

    STRING: 10090.ENSMUSP00000022720

    UniGene: Mm.214746