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  4. Recombinant Mouse F-box/LRR-repeat protein 2 (Fbxl2)

Recombinant Mouse F-box/LRR-repeat protein 2 (Fbxl2)

  • 货号：
    CSB-YP803868MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP803868MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP803868MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP803868MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP803868MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Fbxl2
  • Uniprot No.：
  • 别名：
    Fbxl2F-box/LRR-repeat protein 2; F-box and leucine-rich repeat protein 2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-423
  • 氨基酸序列
    MVFSNSDDGL INKKLPKELL LRIFSFLDIV TLCRCAQISK AWNILALDGS NWQRVDLFNF QTDVEGRVVE NISKRCGGFL RKLSLRGCIG VGDSSLKTFA QNCRNIEHLN LNGCTKITDS TCYSLSRFCS KLKHLDLTSC VSVTNSSLKG ISEGCRNLEY LNLSWCDQIT KEGIEALVRG CRGLKALLLR GCTQLEDEAL KHIQNHCHEL VSLNLQSCSR ITDDGVVQIC RGCHRLQALC LSGCSNLTDA SLTALGLNCP RLQVLEAARC SHLTDAGFTL LARNCHELEK MDLEECVLIT DSTLVQLSIH CPKLQALSLS HCELITDEGI LHLSSSTCGH ERLRVLELDN CLLVTDASLE HLENCRGLER LELYDCQQVT RAGIKRMRAQ LPHVKVHAYF APVTPPPAVA GSGHRLCRCC VIL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium-activated substrate recognition component of the SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex, SCF(FBXL2), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Unlike many F-box proteins, FBXL2 does not seem to target phosphodegron within its substrates but rather calmodulin-binding motifs and is thereby antagonized by calmodulin. This is the case for the cyclins CCND2 and CCND3 which polyubiquitination and subsequent degradation are inhibited by calmodulin. Through CCND2 and CCND3 degradation induces cell-cycle arrest in G(0). SCF(FBXL2) also mediates PIK3R2 ubiquitination and proteasomal degradation thereby regulating phosphatidylinositol 3-kinase signaling and autophagy. PCYT1A monoubiquitination by SCF(FBXL2) and subsequent degradation regulates synthesis of phosphatidylcholine, which is utilized for formation of membranes and of pulmonary surfactant.
  • 基因功能参考文献：
    1. Study demonstrated that TRAF6 plays an important role in the process of aseptic inflammatory loosening caused by periprosthetic debris, and Low intensity pulsed ultrasound was found to inhibit such inflammation through increasing the level of FBXL2, and further increasing ubiquitination of TRAF6. PMID: 28378753
    2. FBXL2 mediates Aurora B ubiquitination and degradation within the midbody, which is sufficient to induce mitotic arrest and apoptosis. PMID: 23928698
    3. FBXL2 is a ubiquitin E3 ligase subunit that triggers mitotic arrest. PMID: 22024926
  • 亚细胞定位：
    Membrane; Lipid-anchor.
  • 数据库链接：

    KEGG: mmu:72179

    STRING: 10090.ENSMUSP00000035090

    UniGene: Mm.386808