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  4. Recombinant Mouse Eukaryotic translation initiation factor 2A (Eif2a)

Recombinant Mouse Eukaryotic translation initiation factor 2A (Eif2a)

  • 货号：
    CSB-YP807369MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP807369MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP807369MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP807369MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP807369MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Eif2a; D3Ertd194eEukaryotic translation initiation factor 2A; eIF-2A) [Cleaved into: Eukaryotic translation initiation factor 2A; N-terminally processed]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-581
  • 氨基酸序列
    MAPSTPLLTV RGSEGLYMVN GPPHFTESTV LPRESGRNCK VYTFSKDGTL FAWSNGEKVN IINVANKGLL HSFDLPKAVC LEFSPNNTVL ATWQPYTTSK DGTAGTPNLQ LYDMKTGACL KSFIQKKMQN WCPSWSDDEI ICARNVNNEV HFFENNNFNT IANKLHLQKV NDFNLSPGTQ PYKVAVYVPG SKGAPSFVRL YQYPNFAGPQ AALANKSFFK ADKVTMLWNK KATAVLVIAS TEVDKTGASY YGEQTLHYIA TNGESAVVQL PKNGPIYDVV WNSSSTEFCA VYGFMPAKAT VFNLKCDPVF DFGTGPRNAA FYSPHGHILV LAGFGNLRGQ MEVWDVKNYK LISKPVASDS TYFAWCPDGE HILTATCAPR LRVNNGYKIW HYTGSLLHKY DVPSNGELWQ VSWQPFLDGI FPAKTIKYQA VPSEVPSEEP KVATAYRPPA LRNKPVTNSK LHEEEPPQNM KPHPGSDKPL SKTALKNQRK HEAKKAAKQE ARSDAAPTPV PQSAPRNTVT QSASGDPEVD KKIKNLKKKL KAIEQLKEQA AAGKQLEKNQ LEKIQKETAL LQELEDLELG V
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner.
  • 基因功能参考文献：
    1. ER stress is induced early in EAE and that modulation of ER stress by inhibition of eIF2alpha-CHOP and activation of XBP-1 in RGC specifically, protects RGC somata and axons and preserves visual function. PMID: 28726788
    2. alpha-asarone abrogated 7beta-hydroxycholesterol-triggered eIF2alpha-CHOP activation, and enhanced macrophage beclin1 dependent autophagy. PMID: 28088783
    3. In response to endoplasmic reticulum stress, activation of PERK coordinates the integrated stress response by phosphorylating eIF2alpha, which is then quickly dephosphorylated by the GADD34 complex. Data imply dual role of the ISR in promoting and inhibiting medulloblastoma tumorigenesis. PMID: 27802424
    4. stress granule formation is a downstream event of eIF2alpha phosphorylation that may not directly protect cells from apoptosis, at least in spermatocytes of seminiferous tubules in early stages. PMID: 28067447
    5. results further extended the understanding of Heme-regulated eIF2alpha kinase on the regulation of non-erythroid lineages and might provide new aspects for preventing and treating Cd-induced detrimental effects PMID: 28700957
    6. Data, including data from studies using cells from knockout mice, suggest that gasotransmitter H(2)S up-regulates eIF2a phosphorylation by inhibiting PPP1CA via persulfidation, which in turn leads to transient suppression of global translation and activation of Atf4 expression. (eIF2a = eukaryotic initiation factor-2alpha; PPP1CA = protein phosphatase 1 catalytic subunit alpha; Atf4 = activating transcription factor 4) PMID: 28637872
    7. Results mechanistically link multiple forms of dystonia and put forth a new overall cellular mechanism for dystonia pathogenesis, impairment of eIF2alpha signaling, a pathway known for its roles in cellular stress responses and synaptic plasticity. PMID: 27939583
    8. The eIF2A knock out mice strain is viable and to date displays no apparent phenotype. PMID: 27686860
    9. The role of dietary zinc in the expression of proteins involved in the endoplasmic reticulum stress response is reported. PMID: 27605406
    10. Thus, during addiction, cocaine hijacks translational control by p-eIF2alpha, initiating synaptic potentiation and addiction-related behaviors. PMID: 26928234
    11. in mouse skeletal muscle stem cells, general repression of translation, mediated by the phosphorylation of translation initiation factor eIF2alpha at serine 51 (P-eIF2alpha), is required to maintain the quiescent state PMID: 26549106
    12. Skeletal muscle-specific eukaryotic translation initiation factor 2alpha phosphorylation controls amino acid metabolism and fibroblast growth factor 21-mediated non-cell-autonomous energy metabolism. PMID: 26487695
    13. These experiments connect embryonic stem cell growth factors to eIF2alpha phosphorylation. PMID: 26406898
    14. PGRN is a key regulator of insulin resistance and PGRN may mediate its effects, at least in part, by inducing ER stress via the PERK-eIF2alpha dependent pathway PMID: 26039714
    15. The CARE-LUC mouse model represents an innovative tool to investigate the eIF2alpha-ATF4 axis and to develop drugs targeting this important pathway in the remediation of related pathologies. PMID: 25921292
    16. inhibition of eIF2alpha and FAK signaling may play an important role in cigarette smoking-induced lung EC apoptosis and emphysema PMID: 24853558
    17. eIF2a phosphorylation is required for the normal function of intestinal Paneth cells and mucosal homeostasis. PMID: 24577114
    18. Hypothalamic eIF2alpha phosphorylation could play a critical role in the onset of anorexia associated with certain diseases. PMID: 24485657
    19. The pathogenesis of cardiomyopathy in Frederich ataxia correlates with the early and persistent eIF2alpha phosphorylation. PMID: 23886890
    20. data underscore the local, innate, pro-inflammatory nature of the response to Clostridium difficile and highlight eIF2alpha phosphorylation and the unfolded protein response PMID: 23668260
    21. eIF2alpha-phosphorylation-attenuated protein synthesis, and not Atf4 mRNA translation, promotes cell survival. PMID: 23624402
    22. The integrated stress response (ISR) by phosphorylating translation initiation factor 2alpha (eIF2alpha) is involved in medulloblastoma development. PMID: 21688289
    23. eIF2alpha is crucial for proper resolution of insulin-induced ER stress. PMID: 21832042
    24. Endoplasmic reticulum stress response mediated by the PERK-eIF2(alpha)-ATF4 pathway is involved in osteoblast differentiation induced by BMP2. PMID: 21135100
    25. eIF2alpha phosphorylation during hypertonic stress promotes apoptosis by sequestration of specific mRNAs in SGs in a process mediated by the cytoplasmic accumulation of hnRNP A1 PMID: 20338999
    26. Double-stranded RNA-dependent protein kinase phosphorylation of the alpha-subunit of eukaryotic translation initiation factor 2 mediates apoptosis PMID: 16717090
    27. functional eIF2alpha played an essential role in PS-341-induced Noxa expression PMID: 16928686
    28. inhibition of eIF2alpha phosphorylation in glucose-deprived cells by the overexpression of dominant-negative PERK or an N-terminal truncation mutant of GADD34 leads to a 53% increase in the rate of total protein synthesis PMID: 18052927
    29. MEK functions to enhance GCN2-dependent eIF2alpha phosphorylation rather than suppressing dephosphorylation PMID: 18287093
    30. eIF2(alphaP)-mediated translation of key hepatic transcriptional regulators of intermediary metabolism contributes to the detrimental consequences of nutrient excess. PMID: 18522833
    31. exogenous ROS inhibit mTOR, eIF2alpha, and eEF2, mTOR and eEF2 were largely refractory to ROS generated under moderate hypoxia (0.5% O(2)). PMID: 18768473
    32. GCN2 in unfertilized mouse eggs is active, resulting in elevated levels of phosphorylated eIF2alpha. PMID: 18996085
    33. GADD34 translation is regulated by a unique 5'UTR uORF mechanism to ensure proper GADD34 expression during eIF2alpha phosphorylation PMID: 19131336
    34. the essential process of eIF2(alphaP) dephosphorylation is the predominant role of PPP1R15 proteins in mammalian development PMID: 19181853
    35. UV-induced eIF2alpha phosphorylation by activation of both PERK and GCN2 via oxidative stress and l-arginine starvation signaling pathways. PMID: 19586904
    36. the accumulation of phosphorylated eIF2alpha and the attenuated phenotype of HSV-2 vhs mutants in MEFs result from a deficiency in ICP34.5 PMID: 19587046

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  • 蛋白家族：
    WD repeat EIF2A family
  • 数据库链接：

    KEGG: mmu:229317

    STRING: 10090.ENSMUSP00000029387

    UniGene: Mm.21617