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  4. Recombinant Mouse Equilibrative nucleoside transporter 1 (Slc29a1), partial

Recombinant Mouse Equilibrative nucleoside transporter 1 (Slc29a1), partial

  • 中文名称：
    小鼠Slc29a1重组蛋白
  • 货号：
    CSB-YP867489MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Slc29a1重组蛋白
  • 货号：
    CSB-EP867489MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Slc29a1重组蛋白
  • 货号：
    CSB-EP867489MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Slc29a1重组蛋白
  • 货号：
    CSB-BP867489MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Slc29a1重组蛋白
  • 货号：
    CSB-MP867489MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Slc29a1
  • Uniprot No.：
  • 别名：
    Slc29a1; Ent1; Equilibrative nucleoside transporter 1; Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter; Equilibrative NBMPR-sensitive nucleoside transporter; Nucleoside transporter; es-type; Solute carrier family 29 member 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Resistant to dipyridamole and dilazep inhibition (anticancer chemotherapeutics drugs).
  • 基因功能参考文献：
    1. Chronic inhibition of ENT1 or by genetic removal of ENT1 enhanced the survival of R6/2 mice. Collectively, adenosine homeostasis and ENT1 expression are altered in Huntington's disease (HD). The inhibition of ENT1 can enhance extracellular adenosine level and be a potential therapeutic approach for treating HD. PMID: 28069792
    2. Study suggests that during sleep-wakefulness, type 1 equilibrative nucleoside transporter is necessary for the linear relationship between EEG markers of waking and the following periods of NREMS, and the adenosine is critical for this regulation. PMID: 26143012
    3. ENBT1 activity is decreased in MVEC in response to the increased superoxide radical that is associated with ischemia/reperfusion injury PMID: 25448155
    4. results establish Augustine as a new blood group system and place SLC29A1 as a new candidate gene for idiopathic disorders characterized with ectopic calcification/mineralization. PMID: 25896650
    5. Findings demonstrate that ENT1 regulates GFAP expression and possibly astrocyte function PMID: 25365803
    6. ENT1-regulated adenosine signaling plays an essential role in lumbar spine and femur bone density PMID: 24586402
    7. findings implicate ENT1 in liver protection from ischemia and reperfusion injury and suggest ENT inhibitors may be of benefit in the prevention or treatment of ischemic liver injury PMID: 23703920
    8. This is the first report of a role for ENT1 in regulating the calcification of soft tissues. ENT1(-/-) mice may be a useful model for investigating pathogenesis and evaluating therapeutics for the prevention of mineralization in DISH and related disord PMID: 23184610
    9. these findings reveal the transcriptional repression of ENT1,2 as an innate protective response during acute pulmonary inflammation. PMID: 23590299
    10. It was shown that the responsiveness of kidney tubules and glomeruli is significantly attenuated in the absence of ENT1, leading to changes of extracellular adenosine levels in the juxtaglomerular apparatus interstitium. PMID: 23269643
    11. This study demonstrated that the A2AR antagonist ZM241385 dampened protein kinase A activity-mediated signaling in the DMS and promoted excessive ethanol drinking in ENT1(+/+) mice PMID: 23467349
    12. Perfusions without sodium drastically reduced the intestinal loss of ribavirin in both wild-type and Ent1(-/-) mice. PMID: 22812541
    13. Mice lacking ENT1 exhibit increased glutamate levels in the nucleus accumbens as well as increased ethanol drinking behaviors. PMID: 21172405
    14. ENT1-null mice possess elevated circulating adenosine levels and reduced cellular uptake resulting in a perpetually cardioprotected phenotype. PMID: 21872611
    15. ENT1 regulates NMDA glutamate receptor-mediated signaling in the nucleus accumbens, which provides a molecular basis that underlies the ethanol-preferring behavior of ENT1 knockout mice. PMID: 21489406
    16. Data suggest that a unified change in nucleoside transporter (NT) gene expression is not required for the normal development and functioning of the ENT1 knockout mice. PMID: 21455274
    17. activation of JNK-cJun pathway negatively regulates mENT1 and suggest that this mechanism might contribute to the development of nucleoside analog-derived drug resistance. PMID: 21145879
    18. ENT1 regulates glutamate uptake activity by altering EAAT2 expression and function, which might be implicated in ethanol intoxication and preference. PMID: 20374202
    19. Nucleoside transporters are important mediators of (18)F-FLT uptake in normal and transformed cells. PMID: 20720035
    20. Our results suggest that adenosine-regulated glutamatergic signaling contributes to a reduced level of alcohol response. PMID: 20085785
    21. ENT1 plays an essential role in cardioprotection, most likely due to its effects in modulating purine nucleoside-dependent signaling. The ENT1-null mouse is a powerful model system for the study of the role of ENTs in the physiology of the cardiomyocyte. PMID: 20035027
    22. PKC delta/epsilon-dependent stimulation of hENT1 transport by a mechanism that may involve activation of transporters at the membrane possibly by post-translational modification of the protein. PMID: 12062437
    23. hENT1 and hENT2 expression was significantly retained in a high percentage of tumors (91% and 96% for hENT1, 84% and 98% for hENT2, in ovarian and cervix carcinomas, respectively). PMID: 15386342
    24. Astrocytes are accordingly well suited for both intense nucleoside metabolism and metabolism-independent uptake to terminate neuromodulator effects of adenosine and guanosine. PMID: 15892125
    25. analysis of interspecies differences in the mitochondrial targeting signal of the equilibrative nucleoside transporter 1 PMID: 16595656
    26. Type 1 equilibrative nucleoside null mice spent more time in the center of an open field, entered more frequently into and spent more time exploring the open arms of a plus maze, and spent more time exploring the light side of a light-dark box. PMID: 17376149
    27. inhibition of CK2 activity, or deletion of Ser(254) from mENT1, enhances transporter affinity for the inhibitor, NBMPR, and reduces the number of ENT1 proteins functioning at the level of the plasma membrane PMID: 17520485
    28. mENT1delta11, a novel altrnative splice variant of ENT1, is characterized. PMID: 18413666
    29. Ent1(+/+) and Ent1(-/-) mice will serve as excellent models to investigate the contribution of Ent1 to the pharmacokinetics and toxicity of ribavirin PMID: 19164463
    30. Ent1 plays a significant role in the oral absorption and erythrocyte distribution of ribavirin. PMID: 19602549

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  • 亚细胞定位：
    Basolateral cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    SLC29A/ENT transporter (TC 2.A.57) family
  • 组织特异性：
    Highly expressed in heart, spleen, lung, liver and testis. Lower level of expression in brain and kidney.
  • 数据库链接：

    KEGG: mmu:63959

    STRING: 10090.ENSMUSP00000063096

    UniGene: Mm.29744