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  4. Recombinant Mouse Ephrin type-B receptor 2 (Ephb2), partial

Recombinant Mouse Ephrin type-B receptor 2 (Ephb2), partial

  • 中文名称：
    小鼠Ephb2重组蛋白
  • 货号：
    CSB-YP007730MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Ephb2重组蛋白
  • 货号：
    CSB-EP007730MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Ephb2重组蛋白
  • 货号：
    CSB-EP007730MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Ephb2重组蛋白
  • 货号：
    CSB-BP007730MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Ephb2重组蛋白
  • 货号：
    CSB-MP007730MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Ephb2
  • Uniprot No.：
  • 别名：
    Ephb2; Epth3; Nuk; Sek3Ephrin type-B receptor 2; EC 2.7.10.1; Neural kinase; Nuk receptor tyrosine kinase; Tyrosine-protein kinase receptor EPH-3; Tyrosine-protein kinase receptor SEK-3) [Cleaved into: EphB2/CTF1; EphB2/CTF2]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Functions in axon guidance during development. Involved in the guidance of commissural axons, that form a major interhemispheric connection between the 2 temporal lobes of the cerebral cortex. Also involved in guidance of contralateral inner ear efferent growth cones at the midline and of retinal ganglion cell axons to the optic disk. In addition to axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses. Upon activation by EFNB1, abolishes the ARHGEF15-mediated negative regulation on excitatory synapse formation. Controls other aspects of development including angiogenesis, palate development and in inner ear development through regulation of endolymph production. Forward and reverse signaling through the EFNB2/EPHB2 complex regulate movement and adhesion of cells that tubularize the urethra and septate the cloaca. May function as a tumor suppressor. May be involved in the regulation of platelet activation and blood coagulation.
  • 基因功能参考文献：
    1. although both EphB2 and EphB3 are necessary for cortical thymic epithelial maturation, the relevance of EphB3 is greater since EphB3-/- thymic cortex exhibits a more severe phenotype than that of EphB2-deficient thymuses PMID: 29027839
    2. Overexpression of EphB2 also rescued the ADDLs-induced depletion of the expression of EphB2 and GluN2B-containing NMDA receptors trafficking in cultured hippocampal neurons. PMID: 28358367
    3. The results of this study indicated that the decrease in spine density in the mPFC was associated with susceptibility to stress, and EphB2 downregulation in the mPFC increased the vulnerability to stress. PMID: 27103064
    4. We here identify that EphB2 receptor tyrosine kinase, which is specifically expressed in glutamatergic neurons, is required for the innate fear responses in the neonatal brain. PMID: 27683910
    5. Here, we identified the interaction sites of the EphB2 FN domain with ADDLs for the first time to develop a small (10 aa) peptide (Pep63) capable of blocking the EphB2-ADDL interaction. PMID: 27881781
    6. EphB4 plays an irreplaceable role in bone regeneration in an inflammatory microenvironment, whereas the functional loss of ephrinB2 can be effectively compensated, most possibly by other ephrins with similar chemical structures PMID: 28077917
    7. s suggest that aging is accompanied by the upregulation of miR-204 in the hippocampus, which downregulates EphB2 and results in reduced surface and total NR1 expression. PMID: 26799631
    8. Findings suggest that a combination of forward and reverse EphB1/2 receptor-mediated signaling contribute to posterior branch of the anterior commissure and corpus callosum axon guidance PMID: 26148571
    9. Results demonstrate that EphB2 reverse signaling plays a unique and requisite role in inhibiting the development of opiate-dependent tolerance in vivo PMID: 26408450
    10. During re-epithelialization ephrin-B1 and its receptor EphB2 are both upregulated in vivo, just for the duration of repair. PMID: 26549443
    11. EphB2 prevents amyloid-beta-induced depletion of cell surface GluN1 requiring the PDZ-binding motif of EphB2. PMID: 26589795
    12. Study concludes that EphB2 forward signaling is required for long-term contextual fear conditioning memory formation. PMID: 26165136
    13. Expression of the Receptor Tyrosine Kinase EphB2 on Dendritic Cells Is Modulated by Toll-Like Receptor Ligation but Is Not Required for T Cell Activation PMID: 26407069
    14. These results suggest a major function for forward signaling through EphB2 and, to a lesser extent, EphB3, in either colonizing progenitor cells or thymic stromal cells. PMID: 25810451
    15. These findings connect the EphB signaling pathway to the regulation of intestinal adenoma initiation via Abl kinase. PMID: 25834110
    16. These findings provide mechanistic evidence for the role of EphB2 in the early progression of cSCC to the invasive stage and identify EphB2 as a putative therapeutic target in this invasive skin cancer. PMID: 25789706
    17. these data demonstrate that EphB2 signaling not only modulates platelet function within a thrombus but is also involved in the regulation of the function of isolated platelets in a contact-independent manner. PMID: 25370417
    18. Osteoblast differentiation and bone strength are maintained by antiapoptotic actions of ephrinB2 signaling. PMID: 24982128
    19. These results shed new light on the mechanism and regulation of EphB2 activation and provide a model on how Eph signaling translates into graded cellular responses. PMID: 24469634
    20. Reelin induces EphB activation. PMID: 23318582
    21. We found that EphB2 signaling reduces the number of induced ipsilateral projections to the medial nucleus of the trapezoid body after unilateral ventral cochlear nucleus removal at postnatal day seven PMID: 23379484
    22. our findings suggest that ephrinB-activated EphB2 receptors recruit a novel Nck/Pak signaling complex to mediate repulsive cortical growth cone guidance. PMID: 23147113
    23. EphB2 and EphB3 reverse signaling are critical for the normal development of the projection from the ventral cochlear nucleus to the contralateral medial nucleus of the trapezoid body. PMID: 22021100
    24. PTPN14 has a role in angiogenesis and/or arteriovenous fate, acting via EphrinB2 and ACVRL1/activin receptor-like kinase 1 PMID: 22233626
    25. EphB-deficient SCID chimeras show profoundly altered thymic epithelial organization that confirms a significant role for EphB2 and EphB3 receptors in the thymocyte-TEC crosstalk. PMID: 21243004
    26. Data show that EphB1, not Ephb2, is the preferred receptor of ephrin-B2 and ephrin-B1 in mediating axon guidance at the optic chiasm despite the coexpression of EphB2 in the same ipsilaterally projecting retinal axons. PMID: 22103419
    27. This study identified many signaling proteins, either known to be part of EphB2 signaling or new for this pathway, which are involved in transient protein-protein interactions upon ephrinB1-Fc stimulation. PMID: 21932443
    28. These results demonstrate that EphB receptors influence auditory cortical responses, and suggest that EphB signaling has multiple functions in auditory system development. PMID: 22022561
    29. EphB2 and EphB1 signallings are key mediators of ventral-temporal retinal ganglion cell axon retinocollicular mapping. PMID: 21847105
    30. Ephrin-B1 interacting with EphB2 controled the migration of dentate progenitor cells into the dorsal half of the developing dentate gyrus. PMID: 21832177
    31. the serine protease neuropsin is critical for stress-related plasticity in the amygdala by regulating the dynamics of the EphB2-NMDA-receptor interaction, the expression of Fkbp5 and anxiety-like behaviour PMID: 21508957
    32. Combination targeting of Dll4/Notch and Ephrin-B2/EphB4 has potential for clinical investigation, providing cumulative efficacy and increased safety over Dll4/Notch inhibition alone. PMID: 21092311
    33. depletion of EphB2 is critical in amyloid-beta-induced neuronal dysfunction; increasing EphB2 levels or function could be beneficial in Alzheimer's disease PMID: 21113149
    34. Data suggest that both EphB2 and EphB3 receptors are required coordinately for pancreatic development. PMID: 21098570
    35. Study identified a RhoA guanine nucleotide exchange factor, Ephexin5, which negatively regulates excitatory synapse development until EphrinB binding to the EphB receptor triggers Ephexin5 phosphorylation, ubiquitination, and degradation. PMID: 21029865
    36. Upon nerve cut, ephrin-B/EphB2 signaling between fibroblasts and Schwann cells results in cell sorting, cell-sorting downstream of EphB2 is mediated by the stemness factor Sox2 through N-cadherin relocalization to Schwann cell-cell contacts. PMID: 20869108
    37. EphB2 receptors were reduced in the hippocampus before the development of impaired object recognition and spatial memory in mouse models of Alzheimer's disease. PMID: 19542617
    38. A trans-synaptic interaction between EphB2 in the presynaptic compartment and ephrin-B3 in the postsynaptic compartment regulates synapse density and the formation of dendritic spines. PMID: 20410461
    39. regulate dendritic spine development via intersectin, Cdc42 and N-WASP. PMID: 12389031
    40. Manipulation of this receptor's regulatory motifs and SH2 binding sites switches MAPK signaling and biological activity PMID: 12486127
    41. EphB1 and EphB3 receptor knockout mice exhibited significantly diminished DPOAE levels as compared to wild-type littermates, indicating that these specific Eph receptors are necessary for normal cochlear function. PMID: 12684184
    42. EphB2 expression appears to be temporally and spatially regulated in the developing mouse GT and differs between sexes. Male pattern of expression can be induced in the female GT by DHT exposure. PMID: 14501676
    43. mutants of EphB2 and EphB3 receptor genes displayed a morphological defect in the ventral midbrain PMID: 15804564
    44. EphB receptors are upstream regulators of FAK in dendritic filopodia and FAK-mediated RhoA activation contributes to assembly of actin filaments in dendritic spines. PMID: 16298995
    45. These data establish EphB receptors as key coordinators of migration and proliferation in the intestinal stem cell niche. PMID: 16777604
    46. Ephb2 induction in hindlimb ischemia depended on Notch activation of Dll1. PMID: 17234965
    47. Differential ephrinB2 (ephB2) reverse signaling modes are evident in two forms of synaptic plasticity: hippocampal long term potentiation, requiring ephB2, and long-term depression, which does not involve ephB2. PMID: 17942722
    48. EphB2 was more frequently detected in large dendritic shafts, in the hippocampus and cerebral cortex. AND in the ventrobasal thalamus, EphB2 was detected most frequently in axon terminals and thin dendritic shafts. PMID: 18410519
    49. Findings reveal a novel feedback loop that promotes EphB2 activation and cell repulsion that is blocked by transcriptional targets of FGFR1. PMID: 19047466
    50. The new structure of EphB2 presented here proves the closing of the receptor cavity after ligand binding, allowing the B-class receptors to use an induced-fit mechanism to recognize and accommodate ligands. PMID: 19193989

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Cell projection, axon. Cell projection, dendrite.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, Ephrin receptor subfamily
  • 组织特异性：
    Expressed in the epithelial dark cells of the inner ear. Expressed in the region of the proximal tubules of the kidney nephron. Expressed in myogenic progenitor cells.
  • 数据库链接：

    KEGG: mmu:13844

    STRING: 10090.ENSMUSP00000101471

    UniGene: Mm.250981