Recombinant Mouse Enteropeptidase (Tmprss15), partial

The recombinant mouse Tmprss15 protein is a fusion protein consists of the mouse Tmprss15 protein (830-1069aa) partnered with the N-terminal 6xHis-SUMO tag. It was produced in the E.coli. This recombinant Tmprss15 protein's purity is greater than 90% determined by SDS-PAGE. After electrophoresis, there is a 44 kDa protein band presented on the gel.

TMPRSS15 have been previously implicated in neuronal function. Besides, novel compound heterozygous TMPRSS15 gene variants could cause enterokinase deficiency. Recently, TMPRSS15 shares high homology in the serine protease domains of TMPRSS2, thus, may participate in the cleavage of the S protein of SARS-CoV-2 during infection. Loss-of-function variants in the TMPRSS15 gene are responsible for EKD. The lack of enterokinase (EK) prevents the activation of trypsinogen, which leads to a disorder of intestinal protein absorption. To date, according to the Human Genome Mutation Database (HGMD), only four variants have been described in the TMPRSS15 gene. The TMPRSS15 gene encodes an enzyme that converts the pancreatic proenzyme trypsinogen to trypsin, which in turn activates other proenzymes, including chymotrypsinogen and procarboxypeptidases. Mutations in TMPRSS15 cause enterokinase deficiency, a malabsorption disorder characterized by diarrhea and failure to thrive.