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  4. Recombinant Mouse Endothelial lipase (Lipg)

Recombinant Mouse Endothelial lipase (Lipg)

  • 中文名称：
    小鼠Lipg重组蛋白
  • 货号：
    CSB-YP895303MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Lipg重组蛋白
  • 货号：
    CSB-EP895303MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Lipg重组蛋白
  • 货号：
    CSB-EP895303MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Lipg重组蛋白
  • 货号：
    CSB-BP895303MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Lipg重组蛋白
  • 货号：
    CSB-MP895303MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Lipg; Endothelial lipase; EC 3.1.1.3; Endothelial cell-derived lipase; EDL
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    21-500
  • 氨基酸序列
    SITTLRPQGS LRDEHHKPTG VPATARPSVA FNIRTSKDPE QEGCNLSLGD SKLLENCGFN MTAKTFFIIH GWTMSGMFES WLHKLVSALQ MREKDANVVV VDWLPLAHQL YTDAVNNTRV VGQRVAGMLD WLQEKEEFSL GNVHLIGYSL GAHVAGYAGN FVKGTVGRIT GLDPAGPMFE GVDINRRLSP DDADFVDVLH TYTLSFGLSI GIRMPVGHID IYPNGGDFQP GCGFNDVIGS FAYGTISEMV KCEHERAVHL FVDSLVNQDK PSFAFQCTDS SRFKRGICLS CRKNRCNNIG YNAKKMRKKR NSKMYLKTRA GMPFKVYHYQ LKVHMFSYNN SGDTQPTLYI TLYGSNADSQ NLPLEIVEKI ELNATNTFLV YTEEDLGDLL KMRLTWEGVA HSWYNLWNEF RNYLSQPSNP SRELYIRRIR VKSGETQRKV TFCTQDPTKS SISPGQELWF HKCQDGWKMK NKTSPFVNLA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Exerts both phospholipase and triglyceride lipase activities. More active as a phospholipase than a triglyceride lipase. Hydrolyzes triglycerides, both with short-chain fatty acyl groups (tributyrin) and long-chain fatty acyl groups (triolein) with similar levels of activity toward both types of substrates. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins.
  • 基因功能参考文献：
    1. FoxA1, FoxA2, and LIPG control the uptake of extracellular lipids for breast cancer growth. PMID: 27045898
    2. inhibitory activity of AOE on pancreatic lipase enzyme was evaluated at concentrations from 60 to 1000 mug/mL. The AOE inhibited the pancreatic lipase with an IC50 = 588.5 mug/mL PMID: 26078966
    3. Hepatic lipase and endothelial lipase influence molecular species of several classes of plasma lipids. PMID: 24777581
    4. Furin has a role as the primary in vivo convertase of ANGPTL3 and endothelial lipase in hepatocytes PMID: 23918928
    5. The role of EL in HDL-associated S1P effects provides new insights into EL action, the responses seen through EL and HDL interaction, and S1P signaling. PMID: 23723371
    6. Endothelial lipase may act as an alternative candidate to provide fatty acids to the heart and regulate cardiac function. PMID: 23460280
    7. Hepatic lipase- and endothelial lipase-deficiency in mice promotes macrophage-to-feces RCT and HDL antioxidant properties. PMID: 23328279
    8. Endothelial lipase supplies cells with free fatty acids and HDL-derived lysophosphatidylcholine and lysophosphatidylethanolamine species resulting in increased cellular triglycerides and phosphatidylcholine (PC) content and decreased PC synthesis. PMID: 23075452
    9. EL expression modulates vascular remodeling as well as plasma HDL-C levels PMID: 22972429
    10. Starvation regulates endothelial lipase expression via SREBP-2. PMID: 23102786
    11. Endothelial lipase plays a role in thyroid and adipocyte biology in addition to its well-known role in endothelial function and HDL metabolism. PMID: 22740344
    12. DEHP exposure did not influence lipase activity, whereas it slightly enhanced UGT activity and exclusively increased CYP4A14 levels in pregnant and/or postpartum dams. PMID: 22159897
    13. targeted disruption of EL increased HDL particles with preserved anti-inflammatory and anti-atherosclerotic functions PMID: 20926433
    14. Statins can reduce EL expression in vitro and in vivo via inhibition of RhoA activity. The inhibition of EL expression in the vessel wall may contribute to the anti-atherogenic effects of statins. PMID: 20045866
    15. The additive effect of hepatic lipase and EL on HDL metabolism but not macrophage reverse cholesterol transport in mice. PMID: 20558822
    16. Data show that endothelial lipase (EL) was upregulated in rat atherosclerosis and taht lipopolysaccharide stimulates EL expression in vitro through NFkappaB activation. PMID: 19931348
    17. Endothelial lipase has a role in HDL metabolism [review] PMID: 12569156
    18. endothelial lipase has a role in regulating HDL levels PMID: 12569160
    19. endothelial lipase has a role in regulating HDL cholesterol levels PMID: 12569161
    20. EL is a major determinant of HDL concentration, structure, and metabolism in mice PMID: 12601178
    21. Human & mouse EL form specific protein-protein interactions, possibly as heterodimers. Inhibition of endogenous mEL by catalytically inactive human ELS149A may be the cause of decreased LDL-phospholipid turnover & increased lipid levels. PMID: 15117821
    22. EL may have indirect atherogenic actions in vivo through its effect on circulating HDL cholesterol and direct atherogenic actions through vascular wall processes such as monocyte recruitment and cholesterol uptake PMID: 15304490
    23. results suggest that endothelial lipase (EL) on the endothelial cell surface can promote monocyte adhesion to the vascular endothelium through the interaction with heparan sulfate proteoglycans PMID: 15485805
    24. The Endothelial lipase (EL) mRNA expression was high in ovaries from pregnant mice but low in ovaries from nonpregnant mice. PMID: 15576837
    25. endothelial lipase activity in adipose tissue and other peripheral tissues might contribute to the tissue uptake of free fatty acids, which could have important implications for the metabolism of plasma lipoproteins PMID: 15630456
    26. EL modulated lipoprotein profile in mice PMID: 16199802
    27. EL expression is upregulated by lipopolysaccharide via TLR4 and promotes the uptake of native LDL by macrophages PMID: 17726294
    28. after stimulation, the protease activity of PC5A is enhanced, as evidenced by the cleavage of the PC5A substrates Lefty, ADAMTS-4, endothelial lipase, and PCSK9. PMID: 18039650
    29. SR-BI-mediated selective uptake of HDL cholesteryl ester is essential for the remodeling of large alpha-migrating HDL particles by EL. PMID: 19136670
    30. Endothelial lipase is a novel modulator of the progression of allergic asthma. PMID: 19168574
    31. EL can modulate plasma HDL-CHOL levels through both its lipolytic and ligand-binding functions in hypertriglyceridemic mice, while lipolytic activity appears to be the main determinant for its effects on HDL metabolism in normolipidemic mice. PMID: 19672025

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    AB hydrolase superfamily, Lipase family
  • 组织特异性：
    Expressed in placenta, lung, liver, testis and spleen.
  • 数据库链接：

    KEGG: mmu:16891

    STRING: 10090.ENSMUSP00000066536

    UniGene: Mm.299647