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  4. Recombinant Mouse Endonuclease 8-like 3 (Neil3)

Recombinant Mouse Endonuclease 8-like 3 (Neil3)

  • 货号：
    CSB-YP809085MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP809085MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP809085MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP809085MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP809085MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Neil3; Endonuclease 8-like 3; EC 3.2.2.-; EC 4.2.99.18; DNA glycosylase FPG2; DNA glycosylase/AP lyase Neil3; Endonuclease VIII-like 3; Nei-like protein 3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-606
  • 氨基酸序列
    VEGPGCTLN GEKIRARVLP GQAVTGVRGT ALQSLLGPAM SPAASLADVA TSAAPMNAKD SGWKLLRLFN GYVYSGVETL GKELFMYFGP RALRIHFGMK GSILINPREG ENRAGASPAL AVQLTRDLIC FYDSSVELRN SVESQQRVRV MEELDICSPK FSFSRAESEV KKQGDRMLCD VLLDQRVLPG VGNIIKNEAL FDSGLHPAVK VCQLSDKQAC HLVKMTRDFS ILFYRCCKAG SAISKHCKVY KRPNCDQCHS KITVCRFGEN SRMTYFCPHC QKENPQCVQV CQLPTRNTEI SWTPRGEDCF TDSVARKSEE QWSCVVCTLI NRPSAKACDA CLTTRPLDSV LKNRENSIAF NNLVKYPCNN FENTHTEVKI NRKTAFGNTT LVLTDLSNKS SALARKKRAN HTIDGESQMF LPTDIGFSDS QHPSKEGINY ITQPSNKVNI SPTVCAQSKL FSSAHKKFKP AHTSATELKS YNSGLSNSEL QTNRTRGHHS KSDGSPLCKM HHRRCVLRVV RKDGENKGRQ FYACSLPRGA QCGFFEWADL SFPFCRHGKR SIMKTVLKIG PNNGKNFFVC PLEKKKQCNF FQWAENGPGM EIVPGC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). Mediates interstrand cross-link repair in response to replication stress: acts by mediating DNA glycosylase activity, cleaving one of the two N-glycosyl bonds comprising the interstrand cross-link, which avoids the formation of a double-strand break but generates an abasic site that is bypassed by translesion synthesis polymerases. In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues.
  • 基因功能参考文献：
    1. Results suggest a novel role for the DNA glycosylase Neil3 in atherogenesis in balancing lipid metabolism and macrophage function, potentially independently of genome-wide canonical base excision repair of oxidative DNA damage. PMID: 27328939
    2. Data suggest that neurogenesis induced by Neil3 repair of oxidative DNA damage protects against prion disease during the clinical phase. PMID: 27886261
    3. NEIL3-dependent modulation of DNA methylation regulates cardiac fibroblast proliferation and thereby affects extracellular matrix modulation after myocardial infarction. PMID: 28052262
    4. These findings demonstrate that deficiency in NEIL3 is associated with increased lymphocyte apoptosis, autoantibodies, and predisposition to autoimmunity. PMID: 27760045
    5. Structural insight into the substrate specificity and marked preference of Neil3 for ssDNA. PMID: 23313161
    6. Our data support the involvement of Neil3 in removal of replication blocks in proliferating cells. PMID: 23305905
    7. Neil3-dependent repair of oxidative DNA damage in neural stem/progenitor cells is required for maintenance of adult neurogenesis to counteract the age-associated deterioration of cognitive performance. PMID: 22959434
    8. Here we report the construction of bicistronic expression vectors for expressing in Escherichia coli the full-length mouse Neil3 (MmuNeil3), its glycosylase domain (MmuNeil3Delta324), as well as the glycosylase domain of human Neil3 (NEIL3Delta324). PMID: 22569481
    9. Neil3 exercises a highly specialized function through accurate molecular repair of DNA in rapidly proliferating cells PMID: 22065741
    10. Neil3 plays a role in repairing 2,6-diamino-4-hydroxy-5-formamidopyrimidine in vivo. PMID: 20185759
    11. NEIL3 is localized in the nuclei; Neil3 mRNA was selectively expressed in thymus, spleen and bone marrow PMID: 16428305
    12. NEIL3 is specifically expressed in brain areas where neurogenesis takes place during development and that its expression is tightly regulated both temporally and spatially. NEIL3 seems to be upregulated in tumor tissues compared to normal tissues. PMID: 19426544

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  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    FPG family
  • 组织特异性：
    Expressed in testis, thymus, spleen and bone marrow. In young mice, expressed at higher levels in thymocytes than splenocytes. At 12 dpc, abundant in the subventricular zone (SVZ) of the lateral ventricles. At 17.5 dpc and P0, expression is limited to dis
  • 数据库链接：

    KEGG: mmu:234258

    STRING: 10090.ENSMUSP00000041909

    UniGene: Mm.281749