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  4. Recombinant Mouse E3 ubiquitin-protein ligase RNF146 (Rnf146)

Recombinant Mouse E3 ubiquitin-protein ligase RNF146 (Rnf146)

  • 中文名称：
    小鼠Rnf146重组蛋白
  • 货号：
    CSB-YP863476MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Rnf146重组蛋白
  • 货号：
    CSB-EP863476MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Rnf146重组蛋白
  • 货号：
    CSB-EP863476MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Rnf146重组蛋白
  • 货号：
    CSB-BP863476MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Rnf146重组蛋白
  • 货号：
    CSB-MP863476MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Rnf146
  • Uniprot No.：
  • 别名：
    Rnf146; E3 ubiquitin-protein ligase RNF146; EC 2.3.2.27; Iduna; RING finger protein 146; RING-type E3 ubiquitin transferase RNF146
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-359
  • 氨基酸序列
    MEMAGCGEID HSINMLPTNK KANESCSNTA PSLTVPECAI CLQTCVHPVS LPCKHVFCYL CVKGASWLGK RCALCRQEIP EDFLDKPTLL SPEELKAASR GNGEYAWYYE GRNGWWQYDE RTSRELEDAF SKGKKNTEML IAGFLYVADL ENMVQYRRNE HGRRRKIKRD IIDIPKKGVA GLRLDCDTNT VNLARESSAD GADSGSAQTG ASVQLAVPSS TRPLTSVDGQ LTSPVTPSPD AGISLEDSFA HLQLSGDSIA ERSHRGEGEE DHESPSSGRV PDTSVEETES DASSDSEDAP VVVAQHSLTQ QRPLVPNGNQ TVADQSDRSG TDRSVAGGGT MSVNVRSRRP DGQCTVTEV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated (PARsylated) proteins and mediates their ubiquitination and subsequent degradation. May regulate many important biological processes, such as cell survival and DNA damage response. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of PARsylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate PARsylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent PARsylated proteins via its WWE domain and mediates their ubiquitination. May regulate TNKS and TNKS2 subcellular location, preventing aggregation at a centrosomal location. Neuroprotective protein. Protects the brain against N-methyl-D-aspartate (NMDA) receptor-mediated glutamate excitotoxicity and ischemia, by interfering with PAR-induced cell death, called parthanatos. Prevents nuclear translocation of AIFM1 in a PAR-binding dependent manner. Does not affect PARP1 activation. Protects against cell death induced by DNA damaging agents, such as N-methyl-N-nitro-N-nitrosoguanidine (MNNG) and rescues cells from G1 arrest. Promotes cell survival after gamma-irradiation. Facilitates DNA repair. Neuroprotective protein. Protects the brain against N-methyl-D-aspartate (NMDA) receptor-mediated glutamate excitotoxicity and ischemia, by interfering with PAR-induced cell death, called parthanatos. Prevents nuclear translocation of AIFM1 in a PAR-binding dependent manner. Does not affect PARP1 activation.
  • 基因功能参考文献：
    1. These findings indicate that RNF146 is required to coordinate beta-catenin signaling within the osteoblast lineage during embryonic and postnatal bone development PMID: 28581440
    2. RANKL represses the transcription of the E3 ubiquitin ligase RNF146 through an NF-kappaB-related inhibitory element in the RNF146 promoter. PMID: 28287403
    3. a new function of RNF146 and tankyrase in stabilizing the Crumbs complex through downregulation of AMOT proteins at the apical membrane, is reported. PMID: 27521426
    4. Modulation of PARP-1-mediated oxidative cell injury by ring finger protein 146 (RNF146) in cardiac myocytes PMID: 24842055
    5. Iduna acts as a potential antioxidant by improving mitochondrial function and inhibiting oxidative stress-induced parthanatos, and these protective effects are dependent on the involvement of ubiquitin-proteasome system. PMID: 23333241

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  • 亚细胞定位：
    Cytoplasm, cytosol. Nucleus.
  • 组织特异性：
    Expressed at relatively high levels in the brain. Also present in spleen, heart, kidney, testis and liver. In the brain, expressed in the cerebellum, hippocampus, striatum, cortex, frontal cortex and, at lowest levels, in olfactory bulb (at protein level)
  • 数据库链接：

    KEGG: mmu:68031

    STRING: 10090.ENSMUSP00000037224

    UniGene: Mm.28930