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  4. Recombinant Mouse E3 ubiquitin-protein ligase RNF135 (Rnf135)

Recombinant Mouse E3 ubiquitin-protein ligase RNF135 (Rnf135)

  • 中文名称：
    小鼠Rnf135重组蛋白
  • 货号：
    CSB-YP880432MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Rnf135重组蛋白
  • 货号：
    CSB-EP880432MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Rnf135重组蛋白
  • 货号：
    CSB-EP880432MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Rnf135重组蛋白
  • 货号：
    CSB-BP880432MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Rnf135重组蛋白
  • 货号：
    CSB-MP880432MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Rnf135
  • Uniprot No.：
  • 别名：
    Rnf135; E3 ubiquitin-protein ligase RNF135; EC 2.3.2.27; RING finger protein 135; RING-type E3 ubiquitin transferase RNF135
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-417
  • 氨基酸序列
    MAAVCSGNAV PVWLSEDDLS CIICQGLLDQ PTTLPCGHSF CLRCLHDLWV SKRGAVDGCP WACPICRKGP LTKPKLHKNP LLQDLVDKYL QAAREVEAGS EPEPAPAPRS APQVTVQKST TNVIQELTDM VRQLVDDVKS LQTQRPNLGS GQDNAQGTPP TDSSSEGEHS LDSPKLVTFS ISQKKIQEIL HNLEEIQEKL QGSVPGRAPP RERVQEMTSS LCLLPDQRRP APRKASHLSL WAISPTFDLR TLSYNLEVSN NSRRVTVSRG DLHTYHWSPQ RFSISQVFCS QALSSGQKYW EVDTRNCSHW AIGVASWGMK RDGMLGRTMD SWCIEWRGPG QFSAWAKMKK TDLQSDLPEV VGVWLDLESG ELAFYAVADH ERLLYECEVS SSSPLHPAFW LYGLSPGNYL EIKQLNT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E2-dependent E3 ubiquitin-protein ligase that functions as a RIG-I/DDX58 coreceptor in the sensing of viral RNAs in cell cytoplasm and the activation of the antiviral innate immune response. Together with the UBE2D3, UBE2N and UB2V1 E2 ligases, catalyzes the 'Lys-63'-linked polyubiquitination of RIG-I/DDX58 oligomerized on viral RNAs, an essential step in the activation of the RIG-I signaling pathway. Through a ubiquitin-independent parallel mechanism, which consists in bridging RIG-I/DDX58 filaments forming on longer viral RNAs, further activates the RIG-I signaling pathway. This second mechanism that synergizes with the ubiquitin-dependent one would thereby allow an RNA length-dependent regulation of the RIG-I signaling pathway. Associated with the E2 ligase UBE2N, also constitutively synthesizes unanchored 'Lys-63'-linked polyubiquitin chains that may also activate the RIG-I signaling pathway. It is not involved in the innate immune response against DNA viruses.
  • 基因功能参考文献：
    1. Together with Riplet, Ube2D3 promotes covalent conjugation of polyubiquitin chains to RIG-I, while Ube2N preferentially facilitates production of unanchored chains. In the presence of these chains, RIG-I induces MAVS aggregation directly on the mitochondria. Data thus reveal two essential mechanisms underlying the activation of RIG-I and MAVS for triggering innate immune signaling in response to viral infection in cells. PMID: 28469175
    2. Riplet-mediated K63-linked polyubiquitination released RIG-I RD autorepression, which allowed the access of positive factors to the RIG-I protein. PMID: 23950712
    3. Riplet is essential for regulating RIG-I-mediated innate immune response against RNA virus infection in vivo. PMID: 21147464
  • 亚细胞定位：
    Cytoplasm. Cytoplasm, Stress granule.
  • 组织特异性：
    Ubiquitously expressed.
  • 数据库链接：

    KEGG: mmu:71956

    STRING: 10090.ENSMUSP00000017839

    UniGene: Mm.22985