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  4. Recombinant Mouse Deoxyribonuclease gamma (Dnase1l3)

Recombinant Mouse Deoxyribonuclease gamma (Dnase1l3)

  • 中文名称：
    小鼠Dnase1l3重组蛋白
  • 货号：
    CSB-YP007052MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Dnase1l3重组蛋白
  • 货号：
    CSB-EP007052MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Dnase1l3重组蛋白
  • 货号：
    CSB-BP007052MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Dnase1l3重组蛋白
  • 货号：
    CSB-MP007052MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Dnase1l3Deoxyribonuclease gamma; DNase gamma; EC 3.1.21.-; DNase I homolog protein DHP2; Deoxyribonuclease I-like 3; DNase I-like 3; Liver and spleen DNase; LS-DNase; LSD
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    26-310
  • 氨基酸序列
    LRLCS FNVRSFGASK KENHEAMDII VKIIKRCDLI LLMEIKDSSN NICPMLMEKL NGNSRRSTTY NYVISSRLGR NTYKEQYAFV YKEKLVSVKT KYHYHDYQDG DTDVFSREPF VVWFHSPFTA VKDFVIVPLH TTPETSVKEI DELVDVYTDV RSQWKTENFI FMGDFNAGCS YVPKKAWQNI RLRTDPKFVW LIGDQEDTTV KKSTSCAYDR IVLCGQEIVN SVVPRSSGVF DFQKAYDLSE EEALDVSDHF PVEFKLQSSR AFTNNRKSVS LKKRKKGNRS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Has DNA hydrolytic activity. Is capable of both single- and double-stranded DNA cleavage, producing DNA fragments with 3'-OH ends. Can cleave chromatin to nucleosomal units and cleaves nucleosomal and liposome-coated DNA. Acts in internucleosomal DNA fragmentation (INDF) during apoptosis and necrosis. The role in apoptosis includes myogenic and neuronal differentiation, and BCR-mediated clonal deletion of self-reactive B cells. Is active on chromatin in apoptotic cell-derived membrane-coated microparticles and thus suppresses anti-DNA autoimmunity. Together with DNASE1, plays a key role in degrading neutrophil extracellular traps (NETs). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation.
  • 基因功能参考文献：
    1. Study reports that DNASE1L3-deficient mice rapidly develop autoantibodies to DNA and chromatin, followed by an systemic lupus erythematosus-like disease. Circulating DNASE1L3 is produced by dendritic cells and macrophages, and its levels inversely correlate with anti-DNA antibody response. PMID: 27293190
    2. DNase gamma, but not CAD, is the effector endonuclease for INDF in cells undergoing necrosis. PMID: 24312463
    3. A comparison of the expression and transport of DNAse1 from rats and DNAse1L3 from mice is reported. PMID: 15796714
    4. These results suggest that DNasegamma provides an alternative mechanism for inducing nuclear changes when the working apoptotic cascade is unsuitable for caspase-activated DNase activation. PMID: 17218958
  • 亚细胞定位：
    Nucleus. Endoplasmic reticulum. Secreted.
  • 蛋白家族：
    DNase I family
  • 组织特异性：
    Expressed at high levels in liver, spleen and testes. Expressed at lower levels in heart, lungs, skeletal muscle and kidney. Not expressed in brain. Predominantly expressed in macrophages; at protein level. Secreted by mononuclear phagocytes.
  • 数据库链接：

    KEGG: mmu:13421

    UniGene: Mm.272258