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  4. Recombinant Mouse Deoxyribonuclease-1 (Dnase1)

Recombinant Mouse Deoxyribonuclease-1 (Dnase1)

  • 中文名称：
    小鼠Dnase1重组蛋白
  • 货号：
    CSB-YP007049MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Dnase1重组蛋白
  • 货号：
    CSB-EP007049MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Dnase1重组蛋白
  • 货号：
    CSB-EP007049MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Dnase1重组蛋白
  • 货号：
    CSB-BP007049MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Dnase1重组蛋白
  • 货号：
    CSB-MP007049MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Dnase1
  • Uniprot No.：
  • 别名：
    Dnase1; Dnl1Deoxyribonuclease-1; EC 3.1.21.1; Deoxyribonuclease I; DNase I
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    23-284
  • 氨基酸序列
    LRIAAFNI RTFGETKMSN ATLSVYFVKI LSRYDIAVIQ EVRDSHLVAV GKLLDELNRD KPDTYRYVVS EPLGRKSYKE QYLFVYRPDQ VSILDSYQYD DGCEPCGNDT FSREPAIVKF FSPYTEVQEF AIVPLHAAPT EAVSEIDALY DVYLDVWQKW GLEDIMFMGD FNAGCSYVTS SQWSSIRLRT SPIFQWLIPD SADTTVTSTH CAYDRIVVAG ALLQAAVVPN SAVPFDFQAE YGLSNQLAEA ISDHYPVEVT LRKI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs. Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA. Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation.
  • 基因功能参考文献：
    1. DNase1 and DNase1-like 3 are independently expressed and thus provide dual host protection against deleterious effects of intravascular neutrophil extracellular traps. PMID: 29191910
    2. HMGN1 binds to CpG island-containing promoters and affects the organization of nucleosomes, DNase I hypersensitivity, and the transcriptional profile of mouse embryonic stem cells and neural progenitors. PMID: 23775126
    3. Early mesangial nephritis initiates a cascade of inflammatory signals that lead to up-regulation of Trap1 and a consequent down-regulation of renal DNaseI by transcriptional interference. PMID: 23273922
    4. silencing of renal DNaseI gene expression initiates a cascade of inflammatory signals including activation of Toll like receptors and Clec4e, leading to progression of both murine and human lupus nephritis PMID: 22479529
    5. Data show that deletion of DNaseI hypersensitive sites does not have an obvious impact on the IgH locus or B cell development. PMID: 21085586
    6. Reduction in renal Dnase1 expression and activity is limited to mice and SLE patients with signs of membranoproliferative nephritis, and may be a critical event in the development of severe forms of lupus nephritis. PMID: 20856893
    7. A conserved sequence located 211 kilobases upstream of class II major histocompatibility complex transcriptional coactivator (CIITA) promoter III is hypersensitive to DNase I. PMID: 20363966
    8. The impact of antibodies to dsDNA, renal Dnase1 and matrix metalloprotease (MMP) mRNA levels and enzyme activities on early and late events in murine lupus nephritis, was determined. PMID: 20041189
    9. serum Dnase1 in cooperation with the plasminogen system guarantees a fast and effective breakdown of chromatin during necrosis by the combined cleavage of DNA as well as of DNA binding proteins. PMID: 15188364
    10. DNase I is essential for kidney injury induced by cisplatin PMID: 15647342
    11. Results describe chromatin disposal during necrosis and the involvement of Deoxyribonuclease 1 in this process with respect to its possible role in the prevention of anti-nuclear auto-immunity. PMID: 16352456
    12. Inhibition of Dnase1 by actin may serve as a self-protection mechanism against premature DNA degradation during cell damage. PMID: 17468836
    13. Overexpression of DNase I in cultured mouse tubular epithelial cells also induced EndoG. Both DNase I and EndoG mediate cisplatin injury to tubular epithelial cells. PMID: 17675668
    14. serum contains two nucleases, DNase1 and DNase1l3, which may substitute or cooperate with each other during DNA degradation, providing effective clearance after exposure or release from dying cells. PMID: 19154352
    15. Gelsolin regulates cardiac remodeling after myocardial infarction through DNase I-mediated apoptosis. PMID: 19246681
    16. The combined down-regulation of Dnase1 and the increased number of apoptotic cells may together be responsible for the transformation of mild mesangial lupus nephritis into severe membranoproliferative, end-stage organ disease. PMID: 19528352
    17. Data demonstrate the importance of DNase I and EndoG in host cell defense against gene and RNA delivery to renal tubular epithelial cells in vitro. PMID: 19558214

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  • 亚细胞定位：
    Secreted. Zymogen granule. Nucleus envelope.
  • 蛋白家族：
    DNase I family
  • 组织特异性：
    Highly expressed in the parotid and submandibular gland as well as in the kidney and duodenum (at protein level). Expressed at intermediate level in the ileum, mesenterial lymph nodes, liver, ventral prostate, epididymis, ovary and stomach (at protein lev
  • 数据库链接：

    KEGG: mmu:13419

    STRING: 10090.ENSMUSP00000006136

    UniGene: Mm.291048