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  4. Recombinant Mouse DNA repair protein RAD52 homolog (Rad52)

Recombinant Mouse DNA repair protein RAD52 homolog (Rad52)

  • 中文名称：
    小鼠Rad52重组蛋白
  • 货号：
    CSB-YP019269MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Rad52重组蛋白
  • 货号：
    CSB-EP019269MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Rad52重组蛋白
  • 货号：
    CSB-EP019269MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Rad52重组蛋白
  • 货号：
    CSB-BP019269MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Rad52重组蛋白
  • 货号：
    CSB-MP019269MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Rad52
  • Uniprot No.：
  • 别名：
    Rad52; DNA repair protein RAD52 homolog
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-420
  • 氨基酸序列
    MAGPEEAVHR GCDNHPPFVG GKSVLLFGQS QYTADEYQAI QKALRQRLGP EYISSRMAGG GQKVCYIEGH RVINLANEMF GYNGWAHSIT QQNVDFVDLN NGKFYVGVCA FVKVQLKDGS YHEDVGYGVS EGLRSKALSL EKARKEAVTD GLKRALRSFG NALGNCILDK DYLRSLNKLP RQLPLDVDLT KTKREDFEPS VEQARYNSCR QNEALGLPKP QEVTSPCRSS PPHDSNIKLQ GAKDISSSCS LAATLESDAT HQRKLRKLRQ KQLQQQFREQ METRRQSHAP AEEVAAKHAA VLPAPPKHST PVTAASELLQ EKVVFPDNLE ENLEMWDLTP DLEDIIKPLC RAEPAQTSAT RTFNNQDSVP HIHCHQKPQE KPGPGHLQTC NTNQHVLGSR EDSEPHRKSQ DLKKRKLDPS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.
  • 基因功能参考文献：
    1. Suppression of Rad52 expression in combination with FANCM knockout drastically reduces cell and tumor growth, suggesting a synthetic lethality interaction between these two genes. PMID: 30022024
    2. Rad52 competes with Ku for binding to S-region double-strand DNA breaks (DSB) free ends, where it facilitates a DSB synaptic process, which favours intra-S region recombination. PMID: 28176781
    3. Results demonstrate that Rad52 depletion increased cell death, decreased myeloid cell frequency, and augmented the activity of CD8+ T cells and NK effectors that ultimately led to reduced tumor growth. These data provide support for the notion of RAD52 as a potential oncogene, implicating a major role for the combined processes of recombinational repair and host immunity in determining risk for Squamous Cell. PMID: 28415565
    4. In cancer-prone, heterozygous APC mutant mice, homozygous deletion of the Rad52 gene suppressed tumor growth and prolonged lifespan. PMID: 27984746
    5. Rad54 has a key function in maintaining genomic integrity of the developing germ cells. PMID: 23949223
    6. Loss of Rad52 partially rescues tumorigenesis and T-cell maturation in Atm-deficient mice. PMID: 15122331
    7. RAD52 modulates the outcome of recombinant HIV-l vector infection by markedly reducing the efficiency of productive integration events PMID: 15297876
    8. Inactivation of RAD52 aggravates X-ray and mitomycine C sensitivity in mice with RAD54 gene degect. PMID: 16009599
    9. Cells expressing Rad52 splice variants favor sister chromatid repair. PMID: 16648471
    10. Results indicate that RAD52 cooperates with OGG1 to repair oxidative DNA damage and enhances the cellular resistance to oxidative stress. PMID: 19506022

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    RAD52 family
  • 数据库链接：

    KEGG: mmu:19365

    STRING: 10090.ENSMUSP00000125502

    UniGene: Mm.149