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  4. Recombinant Mouse DNA-binding protein inhibitor ID-2 (Id2)

Recombinant Mouse DNA-binding protein inhibitor ID-2 (Id2)

  • 货号：
    CSB-YP010967MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP010967MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP010967MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP010967MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP010967MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Id2; Id-2; Idb2DNA-binding protein inhibitor ID-2; Inhibitor of DNA binding 2; Inhibitor of differentiation 2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-134
  • 氨基酸序列
    MKAFSPVRSV RKNSLSDHSL GISRSKTPVD DPMSLLYNMN DCYSKLKELV PSIPQNKKVT KMEILQHVID YILDLQIALD SHPTIVSLHH QRPGQNQASR TPLTTLNTDI SILSLQASEF PSELMSNDSK VLCG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Restricts the CLOCK and ARNTL/BMAL1 localization to the cytoplasm. Plays a role in both the input and output pathways of the circadian clock: in the input component, is involved in modulating the magnitude of photic entrainment and in the output component, contributes to the regulation of a variety of liver clock-controlled genes involved in lipid metabolism.
  • 基因功能参考文献：
    1. It is anegative master modulators of oligodendrocyte differentiation. PMID: 29689424
    2. ID2 is a key mediator of progesterone, which drives luminal lineage differentiation and side branching. PMID: 29950389
    3. phosphorylation of Id2 modulates the degradation of this important cell-cycle regulator. PMID: 26756672
    4. the expression of the transcriptional regulator Id2 is downregulated upon stimulation of invariant natural killer T cells with their cognate antigen. PMID: 26880074
    5. data reveal a previously unrecognized STAT3-mediated immunosuppressive mechanism in DCs and indicate that DC-intrinsic ID2 promotes tumor immunity by modulating tumor-associated CD4(+) T cell responses. PMID: 27678219
    6. Our work identifies a distinct premalignant state and multiple tumorigenic pathways caused by loss of function of Id2 and Id3 PMID: 28258199
    7. Id2 is an important molecular mediator of labyrinthine trophoblast differentiation. PMID: 27168216
    8. Zeb2 regulates commitment to both the cDC2 and pDC lineages through repression of Id2. PMID: 27185854
    9. The s show that Id2 restricts the activity of the Wnt signalling pathway at early stages and prevents precocious differentiation of the embryonic intestinal epithelium. PMID: 28077488
    10. Reduced core body temperature was found in Id2-/- mice. Moreover, in Id2-/- brown adipose tissue, 30 genes including Irs1, PPARs, and PGC-1s were identified as differentially expressed in a sex-specific pattern. PMID: 27144179
    11. this study identifies a role for the E-protein inhibitor Id2 in promoting TH1 cell polarization over follicular T cell polarization PMID: 27213691
    12. ID2 is required not only to antagonize E-proteins during NK cell commitment, but constantly required to titrate E-protein activity to regulate NK cell fitness and responsiveness to IL-15. PMID: 26795246
    13. Data show that knock down of inhibitor of differentiation protein 2 (Id2) attenuates neuroblastoma tumor cells and induces host immunity. PMID: 26079374
    14. overexpression of Id2 in primary alveolar epithelial cells promotes proliferation by inhibiting a retinoblastoma protein/c-Abl interaction leading to greater c-Abl activity. PMID: 25661109
    15. Innate lymphoid cells can mediate immune surveillance, which constantly maintains a proper microbiota, to facilitate early colonization resistance through an ID2-dependent regulation of IL-22. PMID: 25902484
    16. a distinct pattern of hepatic lipid accumulation was observed in Id2-/- males: low lipids on the low-fat diet and steatosis on the high-fat diet. PMID: 25108156
    17. Id2 and Id3 enforce follicular regulatory T-cell checkpoints and control the maintenance and homing of Regulatory T cells. PMID: 24973820
    18. Id2 represses E2A-mediated activation of IL-10 expression in T cells. PMID: 24723679
    19. Data suggest that elevated Id2 expression in embryonic brains can cause deregulated NSC self-renewal, differentiation, and survival that manifest in multiple neurological outcomes in mature brains, including microcephaly, seizures, and brain tumors. PMID: 23390122
    20. expression of the negative regulators of E proteins, Id2 and Id3, defined distinct iNKT cell sublineages. PMID: 24470501
    21. a role for ID2 as a regulator of glucose and lipid metabolism and in the circadian control of feeding/locomotor behavior PMID: 24023810
    22. Id2 is the major modifier of Id3 in limiting Vgamma1.1(+)Vdelta6.3(+) T cell expansion. Conditional knockout of Id2 alone is sufficient to promote a moderate expansion of gammadelta T cells. PMID: 24379125
    23. ID2 and ID3 redundantly promoted iNKT cell lineage specification involving the induction of the signature transcription factor PLZF. ID2 limited iNKT cell numbers by enforcing the postselection checkpoint in conventional thymocytes. PMID: 24244015
    24. It was proposed that Id2 expression in the suprachiasmatic nucleus contributes to maintenance of dynamic circadian oscillations. PMID: 24320178
    25. Irf8 expression in DC9 cells led to an increase in Id2 transcript levels.When coexpressed with Irf8, Batf3 & Id2 had a synergistic effect on classical CD8alpha(+) DC development. PMID: 24227775
    26. Deletion of Id2 and Id3 in T cell progenitors resulted in a partial block at the pre-TCR selection checkpoint and a dramatic increase in numbers of iNKT cells. PMID: 24123680
    27. Expression of transcription factor, inhibitor of DNA-binding protein, a critical factor for natural killer cell development, is impaired in Mysm1-/- NK cells. PMID: 24062447
    28. Findings indicate a function for Id2 in regulating Snai1-mediated repression of integrin beta4. PMID: 23878399
    29. These findings document a novel role for Id2 in the maintenance of midbrain dopamine neurons. PMID: 23264561
    30. Id2-deficient CD8(+) T cells fail to induce sufficient Tbx21 expression. PMID: 23536629
    31. s describe a role for Id2 in both the survival and differentiation of normal CD8 effector and memory populations. PMID: 23325888
    32. STAT5 and STAT3 control expression of Id2 and E2-2 during dendritic cell development. PMID: 23033267
    33. These data suggest a model whereby E protein transcription factor activity favors rapid memory-precursor T-cell formation while their negative regulators, Id2 and Id3, are both required for robust effector CD8( ) T-cell response during infection. PMID: 22585759
    34. Deficiency in Id2 or Id3 resulted in loss of distinct CD8(+) effector and memory populations. PMID: 22057289
    35. Data suggest that JunB contributes to Id2 repression and provides important input in setting the transcriptional program of the EMT and profibrotic responses to TGF-beta. PMID: 22391036
    36. Both the expression of Id2 and Notch are required for the generation of alpha4beta7(+) RORgammat(-) fetal progenitors, but Notch subsequently blocks progression to the RORgammat(+) stage and final maturation of lymphoid tissue inducer cells PMID: 22430492
    37. BMP signaling is primarily responsible for the serum-induced Id2 expression. PMID: 22421219
    38. Data show that spatially-restricted growth defects are localized at the skull base of Id2 KO mice. PMID: 21985998
    39. A role for Id2 in the second heart field contribution at both the arterial and the venous poles of the heart. PMID: 22012595
    40. CpG-rich islands within the Id2 and Id4 genes were bound by PRMT5 and were hypomethylated in PRMT5-deficient cells, suggesting that PRMT5 plays a role in gene silencing during glial cell PMID: 22041901
    41. nuclear retention of Id2 due to RANK signaling plays a decisive role in the survival and differentiation of mammary epithelial cells during mammary gland development PMID: 21947283
    42. Id2 may be a novel target for enhancing sensory axonal regeneration following injuries to the adult spinal cord. PMID: 21679705
    43. Long-term treatment of lower dosage lipopolysaccharide mainly causes upregulation of Id2 protein. PMID: 21110107
    44. Id2 expression delineates differential checkpoints in the genetic program of CD8alpha+ and CD103+ dendritic cell lineages. PMID: 21587207
    45. Results demonstrate that high expression levels of Mad3 in immature B cells induce Id2 expression, which inhibits transcription of genes essential for B cell differentiation. PMID: 20375148
    46. BMP-responsive elements in mouse Id2 and CGCC sequence that contributes to target recognition by Smad proteins were identified. PMID: 20674548
    47. Data demonstrate that benzo(a)pyrene activates PRKAA1/2 and causes ID2 protein loss in trophoblast stem cells in a time- and dose-dependent manner. PMID: 20422711
    48. Data observed physical interactions between Id1, Id2, or Id3 and SMAD3. The data suggest that BMP2 synergistically regulates Fshb transcription with activins, at least in part, through the combined actions of Ids 2 or 3 and SMAD3. PMID: 20463050
    49. Liver metastases in Id2 heterozygotes demonstrated significant decrease of Id2 expression and loss of the remaining Id2 allele, strongly suggesting that Id2 inactivation specifically was required for metastasis in this model PMID: 20127274
    50. Hypoxia-induced ID-2 may contribute to joint destruction in rheumatoid arthritis patients by promoting synovial fibroblast-dependent osteoclastogenesis. PMID: 19950294

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 数据库链接：

    KEGG: mmu:15902

    STRING: 10090.ENSMUSP00000020974

    UniGene: Mm.34871