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  4. Recombinant Mouse Cytoplasmic polyadenylation element-binding protein 3 (Cpeb3)

Recombinant Mouse Cytoplasmic polyadenylation element-binding protein 3 (Cpeb3)

  • 中文名称：
    Recombinant Mouse Cytoplasmic polyadenylation element-binding protein 3(Cpeb3),Yeast
  • 货号：
    CSB-YP766498MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse Cytoplasmic polyadenylation element-binding protein 3(Cpeb3),Yeast
  • 货号：
    CSB-EP766498MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse Cytoplasmic polyadenylation element-binding protein 3(Cpeb3),Yeast
  • 货号：
    CSB-EP766498MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse Cytoplasmic polyadenylation element-binding protein 3(Cpeb3),Yeast
  • 货号：
    CSB-BP766498MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse Cytoplasmic polyadenylation element-binding protein 3(Cpeb3),Yeast
  • 货号：
    CSB-MP766498MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Cpeb3
  • Uniprot No.：
  • 别名：
    Cpeb3; Kiaa0940Cytoplasmic polyadenylation element-binding protein 3; CPE-BP3; CPE-binding protein 3; mCPEB-3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-716
  • 氨基酸序列
    MQDDLLMDKS KTQPQSQQQQ RQQQQQQQQL QPEPGAAEAP STPLSSEIPK PEDSSAVPAL SPASAPPAPN GPDKMQMESP LLPGLSFHQP PQQPPPPQEP TAPGASLSPS FGSTWSTGTT NAVEDSFFQG ITPVNGTMLF QNFPHHVNPV FGGTFSPQIG LAQTQHHQQP PPPAPQPPQP AQPPQAQPSQ QRRSPASPSQ APYAQRSAAA YGHQPIMTSK PSSSSAVAAA AAAAAASSAS SSWNTHQSVN AAWSAPSNPW GGLQAGRDPR RAVGVGVGVG VGVPSPLNPI SPLKKPFSSN VIAPPKFPRA APLTSKSWME DNAFRTDNGN NLLPFQDRSR PYDTFNLHSL ENSLMDMIRT DHEPLKGKHY PNSGPPMSFA DIMWRNHFAG RMGINFHHPG TDNIMALNTR SYGRRRGRSS LFPFEDAFLD DSHGDQALSS GLSSPTRCQN GERVERYSRK VFVGGLPPDI DEDEITASFR RFGPLVVDWP HKAESKSYFP PKGYAFLLFQ EESSVQALID ACLEEDGKLY LCVSSPTIKD KPVQIRPWNL SDSDFVMDGS QPLDPRKTIF VGGVPRPLRA VELAMIMDRL YGGVCYAGID TDPELKYPKG AGRVAFSNQQ SYIAAISARF VQLQHNDIDK RVEVKPYVLD DQMCDECQGT RCGGKFAPFF CANVTCLQYY CEYCWASIHS RAGREFHKPL VKEGGDRPRH VPFRWS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Sequence-specific RNA-binding protein which acts as a translational repressor in the basal unstimulated state but, following neuronal stimulation, acts as a translational activator. In contrast to CPEB1, does not bind to the cytoplasmic polyadenylation element (CPE), a uridine-rich sequence element within the mRNA 3'-UTR, but binds to a U-rich loop within a stem-loop structure. Required for the consolidation and maintenance of hippocampal-based long term memory. In the basal state, binds to the mRNA 3'-UTR of the glutamate receptors GRIA1 and GRIA2 and negatively regulates their translation. Also represses the translation of DLG4, GRIN1 GRIN2A and GRIN2B. When activated, acts as a translational activator of GRIA1 and GRIA2. In the basal state, suppresses SUMO2 translation but activates it following neuronal stimulation. Binds to the 3'-UTR of TRPV1 mRNA and represses TRPV1 translation which is required to maintain normal thermoception. Binds actin mRNA, leading to actin translational repression in the basal state and to translational activation following neuronal stimulation. Negatively regulates target mRNA levels by binding to TOB1 which recruits CNOT7/CAF1 to a ternary complex and this leads to target mRNA deadenylation and decay. In addition to its role in translation, binds to and inhibits the transcriptional activation activity of STAT5B without affecting its dimerization or DNA-binding activity. This, in turn, represses transcription of the STAT5B target gene EGFR which has been shown to play a role in enhancing learning and memory performance. In contrast to CPEB1, CPEB2 and CPEB4, not required for cell cycle progression.
  • 基因功能参考文献：
    1. Topological regulation of synaptic AMPA receptor expression by the RNA-binding protein CPEB3 has been demonstrated. PMID: 27681423
    2. CPEB3 is SUMOylated in hippocampal neurons. CPEB3 regulates SUMO-2 translation. PMID: 26074071
    3. CPEB3 interacts with the actin cytoskeleton in yeast and at mouse synapses. CPEB3 is a functional prion with a tripartite prion domain architecture. PMID: 26074072
    4. The main finding of our study is that CPEB3-mediated protein synthesis is required for long-term hippocampal-based memory storage. PMID: 26074003
    5. Cpeb3 null mice exhibited enhanced short-term fear memory and translationally elevated PSD95, NMDA receptors and GluA1. PMID: 24155305
    6. cleavage of CPEB3 by NMDA-activated calpain 2 accounts for the activity-related translation of CPEB3-targeted RNAs PMID: 22711986
    7. These results suggest a model whereby Neuralized1-dependent ubiquitination facilitates hippocampal plasticity and hippocampal-dependent memory storage by modulating the activity of CPEB3 and CPEB3-dependent protein synthesis and synapse formation. PMID: 22153079
    8. CPEB3 has a novel function in the nucleus as to suppress Stat5b-dependent EGFR gene transcription PMID: 20639532
    9. show that CPEB3 expression is increased in a time-dependent manner during the course of postnatal development, and CPEB3 is localized mostly in the inner retina, including retinal ganglion cells. PMID: 20003455
    10. CPEB3 is expressed in the principal cell layers of the hippocampus PMID: 12871996
    11. CPEB3 is a sequence-specific translational regulatory protein. PMID: 17024188

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Cell junction, synapse. Cell projection, dendrite. Cell junction, synapse, postsynaptic density.
  • 蛋白家族：
    RRM CPEB family
  • 组织特异性：
    Highly expressed in brain (at protein level). In brain, expressed in the hippocampus, granule cells and interneurons of the cerebellum, and mitral cells of the olfactory bulb (at protein level). Detected in the spinal cord and in peripheral dorsal root ga
  • 数据库链接：

    KEGG: mmu:208922

    STRING: 10090.ENSMUSP00000078690

    UniGene: Mm.391176